Characterisation of the LdsRNA encoded mRNA of yeast.

abstract：:The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with ...

journal_title：Current genetics

authors： Oulmouden A,Karst F

更新日期：1991-01-01 00:00:00

abstract：:The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyd...

journal_title：Current genetics

authors： van der Klei IJ,van der Heide M,Baerends RJ,Rechinger KB,Nicolay K,Kiel JA,Veenhuis M

更新日期：1998-07-01 00:00:00

abstract：:Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...

journal_title：Current genetics

authors： L'Homme Y,Stahl RJ,Li XQ,Hameed A,Brown GG

更新日期：1997-04-01 00:00:00

abstract：:cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...

journal_title：Current genetics

authors： Griffioen G,Thevelein JM

更新日期：2002-07-01 00:00:00

abstract：:The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive tempera...

journal_title：Current genetics

authors： Russell PJ,Selker EU,Jackson JA

更新日期：1981-09-01 00:00:00

abstract：:The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...

journal_title：Current genetics

authors： Madsen LH,Kreiberg JD,Gausing K

更新日期：1991-05-01 00:00:00

abstract：:Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 microg...

journal_title：Current genetics

authors： Brunner A,Mendoza V,Tuena de Cobos A

更新日期：1987-01-01 00:00:00

abstract：:A system for the expression of an ATP binding cassette (ABC) transporter from the soybean pathogen Phytophthora sojae is described. Pdr1, an ABC transporter with homology to the pleiotropic drug resistance (PDR) family of transporters, was cloned by primer walking from a P. sojae genomic library. Reverse transcriptase...

journal_title：Current genetics

authors： Connolly MS,Sakihama Y,Phuntumart V,Jiang Y,Warren F,Mourant L,Morris PF

更新日期：2005-12-01 00:00:00

abstract：:We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...

journal_title：Current genetics

authors： Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic G

更新日期：1997-09-01 00:00:00

abstract：:Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...

journal_title：Current genetics

authors： Nostramo R,Herman PK

更新日期：2016-08-01 00:00:00

abstract：:Degenerative processes in the filamentous fungus Podospora anserina are strongly correlated with the instability of the mitochondrial genome. Among the sources of instability is the mobile group-II intron COX1-i1, also called intron alpha, which encodes a protein with a reverse transcriptase activity. In this paper we...

journal_title：Current genetics

authors： Sellem CH,Begel O,Sainsard-Chanet A

更新日期：2000-01-01 00:00:00

abstract：:The complete nucleotide sequence of the Morus mongolica chloroplast (cp) genome was reported and characterized in this study. The cp genome is a circular molecule of 158,459 bp containing a pair of 25,678 bp IR regions, separated by small and large single-copy regions of 19,736 and 87,363 bp, respectively. The number ...

journal_title：Current genetics

authors： Kong W,Yang J

更新日期：2016-02-01 00:00:00

abstract：:A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...

journal_title：Current genetics

authors： Thornewell SJ,Peery RB,Skatrud PL

更新日期：1995-12-01 00:00:00

abstract：:Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referre...

journal_title：Current genetics

authors： Chang YS,Dubin RA,Perkins E,Forrest D,Michels CA,Needleman RB

更新日期：1988-09-01 00:00:00

abstract：:Saccharomyces cerevisiae adapts to oxidative, osmotic stress and nutrient deprivation through transcriptional changes, decreased proliferation, and entry into other developmental pathways such as pseudohyphal formation and sporulation. Inositol pyrophosphates are necessary for these cellular responses. Inositol pyroph...

journal_title：Current genetics

authors： Morrissette VA,Rolfes RJ

更新日期：2020-10-01 00:00:00

abstract：:Ubiquitination/deubiquitination pathways are now recognized as key components of gene regulatory mechanisms in eukaryotes. The major transcriptional repressor for carbon catabolite repression in Aspergillus nidulans is CreA, and mutational analysis led to the suggestion that a regulatory ubiquitination/deubiquitinatio...

journal_title：Current genetics

authors： Alam MA,Kamlangdee N,Kelly JM

更新日期：2016-09-02 00:00:00

abstract：:The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...

journal_title：Current genetics

authors： Patel D,Evans IH,Bevan EA

更新日期：1990-04-01 00:00:00

abstract：:Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produ...

journal_title：Current genetics

authors： Whisson SC,Drenth A,Maclean DJ,Irwin JA

更新日期：1994-12-01 00:00:00

abstract：:A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or co...

journal_title：Current genetics

authors： Judelson HS,Coffey MD,Arredondo FR,Tyler BM

更新日期：1993-03-01 00:00:00

abstract：:Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are...

journal_title：Current genetics

authors： Sabouri N

更新日期：2017-08-01 00:00:00

abstract：:Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...

journal_title：Current genetics

authors： Goedegebuur F,Fowler T,Phillips J,van der Kley P,van Solingen P,Dankmeyer L,Power SD

更新日期：2002-05-01 00:00:00

abstract：:DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...

journal_title：Current genetics

authors： Raeder U,Broda P

更新日期：1984-09-01 00:00:00

abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

journal_title：Current genetics

authors： Munz P,Dorsch-Häsler K,Leupold U

更新日期：1983-04-01 00:00:00

abstract：:Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

journal_title：Current genetics

authors： Jakobsen KP,Andersen AH,Bjergbæk L

更新日期：2019-10-01 00:00:00

abstract：:Work in recent years has produced indirect evidence to support the view that the phenomenon of suppressiveness in yeast is the result of the ability of the petite mtDNA to out-replicate the wild-type genome. We have developed a method, based on fluorography of gels containing restriction fragments of radioactively lab...

journal_title：Current genetics

authors： Chambers P,Gingold E

更新日期：1986-01-01 00:00:00

abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

journal_title：Current genetics

authors： Destombe C,Douglas SE

更新日期：1991-05-01 00:00:00

abstract：:A screening procedure for cell cycle mutants among is mutants of Physarum has been developed for unsynchronized microplasmodia. The synchronization of the microplasmodia and the ratio of pre- to post-mitotic nuclei were evaluated after a transient shift-up to the non-permissive temperature. In contrast with wild type ...

journal_title：Current genetics

authors： Wright M,Del Castillo L,Oustrin ML

更新日期：1980-04-01 00:00:00

abstract：:The ribosomal DNA repeat unit of Sphaeronaemella fimicola was found to be a 13.7-kb tandem repeat with a relatively long nontranscribed spacer (NTS) and an unusually compact ribosomal RNA gene cluster. The DNA sequence of an 850-bp PCR amplification product containing the 3' end of the small subunit rRNA (SSrRNA) gene...

journal_title：Current genetics

authors： Hausner G,Klassen GR,Reid J

更新日期：1993-01-01 00:00:00

abstract：:By using the fluorochrome 4'-6-diamidino-2-phenylindole (DAPI) to stain DNA one can follow the pattern of events affecting plastid DNA which occur in the formation and maturation of individual zygotes of the green flagellate Chlamydomonas moewusii. This species, like C. reinhardi, expresses uniparental inheritance of ...

journal_title：Current genetics

authors： Coleman AW,Maguire MJ

更新日期：1983-06-01 00:00:00

abstract：:In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

journal_title：Current genetics

authors： Meissner M,Stark K,Cresnar B,Kirk DL,Schmitt R

更新日期：1999-12-01 00:00:00