Abstract:
:A procedure for stable transformation was developed for Phytophthora megasperma f. sp. glycinea, an oomycete pathogen of soybean. Transformants were obtained using a bacterial hygromycin resistance gene fused to a promoter and terminator from the ham34 gene of another oomycete, Bremia lactucae. Vector DNA, alone or complexed to cationic liposomes, was introduced into protoplasts using polyethylene glycol and CaCl2. DNA and RNA hybridization, and phosphotransferase assays, confirmed the presence and expression of vector DNA in the transformants. Hybridization to electrophoretically separated chromosomes of P. m. glycinea showed that vector DNA had integrated into only one chromosome in four transformants, and into multiple chromosomes in one transformant.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Judelson HS,Coffey MD,Arredondo FR,Tyler BMdoi
10.1007/BF00351498subject
Has Abstractpub_date
1993-03-01 00:00:00pages
211-8issue
3eissn
0172-8083issn
1432-0983journal_volume
23pub_type
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