Gene replacement in Penicillium roqueforti.

abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

journal_title：Current genetics

authors： Destombe C,Douglas SE

更新日期：1991-05-01 00:00:00

abstract：:Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...

journal_title：Current genetics

authors： Behringer MG,Hall DW

更新日期：2016-08-01 00:00:00

abstract：:Studies of motility in Physarum polycephalum have concentrated on the well-defined actomyosin system in plasmodia. It is clear from recent genetic studies in lower eukaryotes that myosin is involved in a number of physiological processes in addition to the contractile functions previously ascribed to the classical typ...

journal_title：Current genetics

authors： Murray M,Foxon J,Sweeney F,Orr E

更新日期：1994-02-01 00:00:00

abstract：:In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between he...

journal_title：Current genetics

authors： Chevanne D,Bastiaans E,Debets A,Saupe SJ,Clavé C,Paoletti M

更新日期：2009-02-01 00:00:00

abstract：:The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

journal_title：Current genetics

authors： Dzikowska A,Swianiewicz M,Talarczyk A,Wisniewska M,Goras M,Scazzocchio C,Weglenski P

更新日期：1999-03-01 00:00:00

abstract：:SecA is one of seven Sec proteins that comprise the prokaryotic protein translocation apparatus. A chloroplast-encoded secA gene has been identified from the unicellular chromophytic alga Pavlova lutherii. The gene predicts a protein that is related to the SecA proteins of Escherichia coli and Bacillus subtilis. The p...

journal_title：Current genetics

authors： Scaramuzzi CD,Hiller RG,Stokes HW

更新日期：1992-11-01 00:00:00

abstract：:The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a pote...

journal_title：Current genetics

authors： Jia J,Wheals A

更新日期：2000-12-01 00:00:00

abstract：:A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

journal_title：Current genetics

authors： Hooley P,Shawcross SG,Strike P

更新日期：1988-11-01 00:00:00

abstract：:Inorganic phosphate is an essential nutrient because it is required for the biosynthesis of nucleotides, phospholipids and metabolites in energy metabolism. During phosphate starvation, phosphatases play a major role in phosphate acquisition by hydrolyzing phosphorylated macromolecules. In Saccharomyces cerevisiae, PH...

journal_title：Current genetics

authors： Yadav KK,Singh N,Rajasekharan R

更新日期：2016-05-01 00:00:00

abstract：:We describe a new nuclear gene, CBT1 (Cytochrome B Termination), specifically involved in the generation of mature mRNA of cytochrome b in yeast mitochondria. Disruption of CBT1 (corresponding to ORF YKL 208W) results in a respiratory deficiency (no growth on acetate and ethanol, a reduced growth on glycerol, and a mo...

journal_title：Current genetics

authors： Rieger KJ,Aljinovic G,Lazowska J,Pohl TM,Slonimski PP,Aljinovic G

更新日期：1997-09-01 00:00:00

abstract：:Chromosomes of two closely related yeast strains, the amylolytic Saccharomyces diastaticus and the non-amylolytic Saccharomyces cerevisiae, were resolved by pulsed field gel electrophoresis (PFGE) and orthological field alteration gel electrophoresis (OFAGE). Electrophoretic karyotypes of these two strains are identic...

journal_title：Current genetics

authors： Pretorius IS,Marmur J

更新日期：1988-07-01 00:00:00

abstract：:Spontaneous and double-strand break (DSB)-induced gene conversion in Saccharomyces cerevisiae was assayed using non-tandem chromosomal direct repeat crosses and plasmid x chromosome crosses. Each cross involved identical ura3 alleles marked with phenotypically silent restriction fragment length polymorphic (RFLP) muta...

journal_title：Current genetics

authors： Cho JW,Khalsa GJ,Nickoloff JA

更新日期：1998-10-01 00:00:00

abstract：:Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referre...

journal_title：Current genetics

authors： Chang YS,Dubin RA,Perkins E,Forrest D,Michels CA,Needleman RB

更新日期：1988-09-01 00:00:00

abstract：:The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

journal_title：Current genetics

authors： Howe CJ

更新日期：1985-01-01 00:00:00

abstract：:Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...

journal_title：Current genetics

authors： Dobson MJ,Futcher AB,Cox BS

更新日期：1980-12-01 00:00:00

abstract：:In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...

journal_title：Current genetics

authors： White D,Chen W

更新日期：2006-04-01 00:00:00

abstract：:In previous work, four genes involved in mating-type determination were cloned from reference strains of Pyrenopeziza brassicae; three genes, PAD1, PMT1, and PHB1 (re-named henceforth as MAT-1-1, MAT-1-4, and MAT1-3, respectively), are encoded by the MAT-1 idiomorph, and one gene, PHB2 (re-named MAT-2), by the corresp...

journal_title：Current genetics

authors： Singh G,Dyer PS,Ashby AM

更新日期：1999-11-01 00:00:00

abstract：:By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...

journal_title：Current genetics

authors： Michel F

更新日期：1984-05-01 00:00:00

abstract：:The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...

journal_title：Current genetics

authors： Patel D,Evans IH,Bevan EA

更新日期：1990-04-01 00:00:00

abstract：:The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required ...

journal_title：Current genetics

authors： Malone RE,Jordan K,Wardman W

更新日期：1985-01-01 00:00:00

abstract：:We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates from F. solani f. sp. cucurbitae races 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Fo...

journal_title：Current genetics

authors： Crowhurst RN,Hawthorne BT,Rikkerink EH,Templeton MD

更新日期：1991-11-01 00:00:00

abstract：:Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...

journal_title：Current genetics

authors： Hasunuma K,Shinohara Y

更新日期：1985-01-01 00:00:00

abstract：:We find three interesting characteristics of the genes encoding the ribosomal RNA (rRNA) in the basidiomycete Coprinus cinereus. First, there are only 60 to 90 copies of the genes, fewer than in other fungi. Second, the genes are organized in an unusual arrangement. The 5S rRNA genes are located in the repeat unit whi...

journal_title：Current genetics

authors： Rhodes Cassidy J,Moore D,Lu BC,Pukkila PJ

更新日期：1984-10-01 00:00:00

abstract：:During evolution, most of the bacterial genes from the ancestral endosymbiotic alpha-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. A crucial evolutionary step was the establishment of macromolecule import systems to allow the come back of proteins and RNAs int...

journal_title：Current genetics

authors： Duchêne AM,Pujol C,Maréchal-Drouard L

更新日期：2009-02-01 00:00:00

abstract：:Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

journal_title：Current genetics

authors： Challa K,Shinohara M,Shinohara A

更新日期：2019-08-01 00:00:00

abstract：:Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...

journal_title：Current genetics

authors： Rosario-Cruz Z,Boyd JM

更新日期：2016-02-01 00:00:00

abstract：:Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

journal_title：Current genetics

authors： Goodwin TJ,Ormandy JE,Poulter RT

更新日期：2001-04-01 00:00:00

abstract：:A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...

journal_title：Current genetics

authors： Wang Z,Tarawneh KA,Free SJ

更新日期：1993-01-01 00:00:00

abstract：:A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...

journal_title：Current genetics

authors： Ferrero I,Rambaldelli R,Genga AM,Donnini C,Puglisi PP

更新日期：1984-08-01 00:00:00

abstract：:The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic ch...

journal_title：Current genetics

authors： Swinnen E,Rosseels J,Winderickx J

更新日期：2005-07-01 00:00:00