Abstract:
:The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required for recombination and repair processes in yeast, we have isolated extragenic revertants of rad50-4 which restore the ability to grow in the presence of MMS. Evidence from segregation indicates the extragenic revertants fall into at least five loci. Two of them reduce sporulation and spore viability at high temperature; another mutation confers a spontaneous hyperrec phenotype on mitotic cells. Thus, at least three revertants are candidates for mutations which affect recombination functions.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Malone RE,Jordan K,Wardman Wdoi
10.1007/BF00434050subject
Has Abstractpub_date
1985-01-01 00:00:00pages
453-61issue
6eissn
0172-8083issn
1432-0983journal_volume
9pub_type
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