Amphimeric mitochondrial genomes of petite mutants of yeast. II. A model for the amplification of amphimeric mitochondrial petite DNA.

abstract：:Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...

journal_title：Current genetics

authors： Lee H,Lee DG

更新日期：2019-02-01 00:00:00

abstract：:Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...

journal_title：Current genetics

authors： Hasunuma K,Shinohara Y

更新日期：1985-01-01 00:00:00

abstract：:The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. C...

journal_title：Current genetics

authors： Katsura K,Suzuki F,Miyashita SI,Nishi T,Hirochika H,Hashiba T

更新日期：1997-12-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...

journal_title：Current genetics

authors： Sybirna K,Guiard B,Li YF,Bao WG,Bolotin-Fukuhara M,Delahodde A

更新日期：2005-03-01 00:00:00

abstract：:A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...

journal_title：Current genetics

authors： Vainio AE,Torkkeli HT,Tuusa T,Aho SA,Fagerström BR,Korhola MP

更新日期：1993-07-01 00:00:00

abstract：:A gene of Puccinia graminis f. sp. tritici, coding for the translation elongation factor 1 alpha (EF-1 alpha), was isolated from a P. graminis genomic library using the EF-1 alpha gene sequence of Absidia glauca. The coding region of 1389 nucleotides encodes a polypeptide of 463 amino acids and is interrupted by eight...

journal_title：Current genetics

authors： Schillberg S,Gross P,Tiburzy R

更新日期：1995-03-01 00:00:00

abstract：:In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...

journal_title：Current genetics

authors： Grimm C,Böhl L,Osiewacz HD

更新日期：2015-02-01 00:00:00

abstract：:Mutations in the RecA-like genes RAD51 and RAD57 reduce the frequency of gene conversion/reciprocal exchange between inverted repeats 7-fold. However, they enhance the frequency of deletions between direct repeats 5-12-fold. These induced deletions are RAD1- and RAD52-dependent. On the basis of these results it is pro...

journal_title：Current genetics

authors： Aguilera A

更新日期：1995-03-01 00:00:00

abstract：:The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...

journal_title：Current genetics

authors： Lin Y,Smith GR

更新日期：1995-04-01 00:00:00

abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...

journal_title：Current genetics

authors： Huynh AD,Leblon G,Zickler D

更新日期：1986-01-01 00:00:00

abstract：:In order to facilitate the process of epitope-tagging of yeast proteins, we have constructed two Saccharomyces cerevisiae-Escherichia coli shuttle vectors that allow fusion of a sequence encoding an epitope of the human c-myc protein at the 3' end of any gene. An example of the use of this technique is presented. ...

journal_title：Current genetics

authors： Reisdorf P,Maarse AC,Daignan-Fornier B

更新日期：1993-02-01 00:00:00

abstract：:Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...

journal_title：Current genetics

authors： Harrington TC,McNew DL

更新日期：1997-07-01 00:00:00

abstract：:In Saccharomyces cerevisiae, the HGS2-1 allele confers sensitivities to inorganis mercury (Ono and Sakamoto 1985) and to excess fermentable sugars such as glucose (Sakamoto et al. 1985); exogenous tyrosine antagonizes both inorganic mercury and excess glucose. In this study, the inorganic mercury sensitive strain has ...

journal_title：Current genetics

authors： Ono B,Sakamoto E,Yamaguchi K

更新日期：1987-01-01 00:00:00

abstract：:By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...

journal_title：Current genetics

authors： Fukuda M,Harada Y,Imahori S,Fukumasa-Nakai Y,Hayashi Y

更新日期：1995-05-01 00:00:00

abstract：:In the wood destroying fungus Schizophyllum commune, a well known subject for genetic studies, fruit bodies are produced not only in the course of the sexual cycle but also asexually. Sexual fruiting requires the establishment of a dikaryon which is under the control of the incompatibility factors A and B. Asexual fru...

journal_title：Current genetics

authors： Esser K,Saleh F,Meinhardt F

更新日期：1979-12-01 00:00:00

abstract：:The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. The infection process of Fol comprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virule...

journal_title：Current genetics

authors： Yun Y,Zhou X,Yang S,Wen Y,You H,Zheng Y,Norvienyeku J,Shim WB,Wang Z

更新日期：2019-06-01 00:00:00

abstract：:The wheat mitochondrial (mt) cox3 has been localized and sequenced. The gene exists as a single copy in the wheat mt master chromosome and is transcribed into a single 1.2 kb RNA, whose extremities have been mapped. Comparison of the wheat and Oenothera cox3 sequences gives ambiguous indications concerning the amino a...

journal_title：Current genetics

authors： Gualberto JM,Domon C,Weil JH,Grienenberger JM

更新日期：1990-01-01 00:00:00

abstract：:The zinc finger transcription factor Crz1 is an important downstream regulator of calcium-dependent signal transduction pathways in many organisms. The function of Crz1 in the wheat-head blight pathogen Fusarium graminearum remains unclear. In this study, we identified and functionally characterised FgCrz1A, a potenti...

journal_title：Current genetics

authors： Chen L,Tong Q,Zhang C,Ding K

更新日期：2019-02-01 00:00:00

abstract：:Five haploid monokaryons of the basidiomycete Coprinus cinereus were already known to be unable to form sclerotia (asexual resting structures) on the vegetative monokaryotic mycelium. Genetic analyses had shown that four distinct genes (symbolised scl were represented, all being "recessive" to their sclerotium-produci...

journal_title：Current genetics

authors： Moore D

更新日期：1981-05-01 00:00:00

abstract：:Most organisms prefer to utilize glucose as a carbon source. Accordingly, the expression of genes involved in the catabolism of other carbon sources is repressed by the presence of glucose in a process known as (carbon) catabolite repression. However, much less is known about the relationships between "poor" carbon so...

journal_title：Current genetics

authors： Simpson-Lavy K,Kupiec M

更新日期：2019-12-01 00:00:00

abstract：:Many species are able to hybridize, but the sterility of these hybrids effectively prevents gene flow between the species, reproductively isolating them and allowing them to evolve independently. Yeast hybrids formed by Saccharomyces cerevisiae and Saccharomyces paradoxus parents are viable and able to grow by mitosis...

journal_title：Current genetics

authors： Ono J,Greig D

更新日期：2020-06-01 00:00:00

abstract：:A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...

journal_title：Current genetics

authors： Thornewell SJ,Peery RB,Skatrud PL

更新日期：1995-12-01 00:00:00

abstract：:Two cob- deletion mutants are characterized. One of them, M9410, is deleted for 911 bp of the noncoding sequences only which separate tRNAGlu and cob exon 1; it thus lacks most of the sequence encoding the 957 bp long cob leader (Bonitz et al. 1982) and some 20 bp 5' to it. The end points of this deletion coincide wit...

journal_title：Current genetics

authors： Dobres M,Gerbl-Rieger S,Schmelzer C,Mueller MW,Schweyen RJ

更新日期：1985-01-01 00:00:00

abstract：:Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

journal_title：Current genetics

authors： Challa K,Shinohara M,Shinohara A

更新日期：2019-08-01 00:00:00

abstract：:As yeast colonies ceased growth, cells at the edge of these colonies transited from the cell division cycle into meiosis at high efficiency. This transition occurred remarkably synchronously and only at late stages of colony maturation. The transition occurred on medium containing acetate or low concentrations of gluc...

journal_title：Current genetics

authors： Purnapatre K,Honigberg SM

更新日期：2002-10-01 00:00:00

abstract：:The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...

journal_title：Current genetics

authors： Harrer R,Schwank S,Schüller HJ,Schweizer E

更新日期：1993-07-01 00:00:00

abstract：:The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate trans...

journal_title：Current genetics

authors： Lawson MR,Berger JM

更新日期：2019-06-01 00:00:00

abstract：:We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isole...

journal_title：Current genetics

authors： Okada H,Toh-e A

更新日期：1992-02-01 00:00:00

abstract：:Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We he...

journal_title：Current genetics

authors： Tsai YY,Ohashi T,Kanazawa T,Polburee P,Misaki R,Limtong S,Fujiyama K

更新日期：2017-05-01 00:00:00

abstract：:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...

journal_title：Current genetics

authors： Viterbo A,Montero M,Ramot O,Friesem D,Monte E,Llobell A,Chet I

更新日期：2002-11-01 00:00:00