Abstract:
:The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive temperature and vice versa. The results showed that the primary cold-sensitive defect in crib-1 is in the function, rather than the synthesis, of a molecule needed for the production of the 17S rRNA component of the 37S subunit. Pulse-labeling experiments showed that at 10 °C crib-1 synthesizes the 2.4-Mdal pre-rRNA molecule which contains the sequences for the 17S, 5.8S and 25S rRNA species, but that processing of this molecule is defective such that relatively few stable 17S rRNA molecules accumulate.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Russell PJ,Selker EU,Jackson JAdoi
10.1007/BF00376778subject
Has Abstractpub_date
1981-09-01 00:00:00pages
1-5issue
1eissn
0172-8083issn
1432-0983journal_volume
4pub_type
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