Abstract:
:A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acids and has an estimated molecular mass of 55.4 kDa. CmFad9p is a unique delta-9 fatty acid desaturase among plants, in that it is fused with the cytochrome b5 domain at its carboxyl terminus. This is characteristic of yeast acyl-CoA desaturase. Genomic Southern hybridization suggested that the C. merolae genome contains a single gene for delta-9 fatty acid desaturase of the animal and fungal type. Southern hybridization combined with pulsed-field gel electrophoresis revealed that CmFAD9 is probably located on chromosome XI of the 17 C. merolae chromosomes. A 1.6-kb product of this gene was transcribed throughout a light/dark synchronization culture. The discovery of CmFAD9 indicates the existence of a novel type of plant delta-9 fatty acid desaturase that may function in the endoplasmic reticulum, but not in the plastid.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Itoh R,Toda K,Takahashi H,Takano H,Kuroiwa Tdoi
10.1007/s002940050323subject
Has Abstractpub_date
1998-03-01 00:00:00pages
165-70issue
3eissn
0172-8083issn
1432-0983journal_volume
33pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0697-4
更新日期:2017-10-01 00:00:00
abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351689
更新日期:1992-04-01 00:00:00
abstract::We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050124
更新日期:1996-08-01 00:00:00
abstract::The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940000130
更新日期:2000-07-01 00:00:00
abstract::Antimycin-resistant (AR) mutants of the yeast Kluyveromyces lactis, obtained either spontaneously or after manganese treatment, were isolated and genetically characterized. Most of the mutants obtained after manganese mutagenesis and two spontaneous mutants, tolerated high antimycin concentrations (more than 10 microg...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384609
更新日期:1987-01-01 00:00:00
abstract::Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0808-x
更新日期:2018-08-01 00:00:00
abstract::A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02426965
更新日期:1996-05-01 00:00:00
abstract::The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0010-9
更新日期:2005-09-01 00:00:00
abstract::The mother-bud neck is defined as the boundary between the mother cell and bud in budding microorganisms, wherein sequential morphological events occur throughout the cell cycle. This study was designed to quantitatively investigate the morphology of the mother-bud neck in budding yeast Saccharomyces cerevisiae. Obser...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0872-2
更新日期:2019-02-01 00:00:00
abstract::La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312776
更新日期:1991-07-01 00:00:00
abstract::Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is responsible for the re...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420614
更新日期:1986-01-01 00:00:00
abstract::Mitochondrial RNA from grande yeast was analyzed by electrophoresis on agarose-urea, acrylamide-urea, and methyl mercuric hydroxide-agarose gels. These gel systems display more than 40 RNA bands that copurify with mitochondria; these bands are not present in cytoplasmic RNA preparations. Analysis of molecular weight o...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00446962
更新日期:1980-02-01 00:00:00
abstract::NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315785
更新日期:1995-07-01 00:00:00
abstract::In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418411
更新日期:1986-01-01 00:00:00
abstract::Yeast ornithine transaminase is known to be induced by arginine and ornithine, through the action of regulatory elements common to arginase induction. We show here that it is subject to a second induction circuit, that which is responsible for urea amidolyase and urea permease induction by allophanate and defined by t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376788
更新日期:1981-09-01 00:00:00
abstract::In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between he...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0227-5
更新日期:2009-02-01 00:00:00
abstract::nam3 and R705, yeast nuclear omnipotent suppressors of mitochondrial mit- mutations, reverse the superimposed spectrum of trans-recessive splicing defects by affecting the protein composition of the small mitoribosomal subunit. Analysis of the suppressor's interaction suggests that suppression results from mutations i...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365647
更新日期:1988-02-01 00:00:00
abstract::The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00417817
更新日期:1984-04-01 00:00:00
abstract::How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100227
更新日期:2001-08-01 00:00:00
abstract::DNA-damage tolerance (DDT) is employed by eukaryotes to deal with replication blocks on the template strand, and is divided into two parallel pathways that are activated by sequential ubiquitination of proliferating cell nuclear antigen (PCNA) at the Lys164 residue. Rad6-Rad18-mediated PCNA monoubiquitination promotes...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-017-0753-0
更新日期:2018-04-01 00:00:00
abstract::Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050118
更新日期:1996-07-31 00:00:00
abstract::Fourteen nuclear complementation groups of mutants that specifically affect the three mitochondrially-encoded subunits of yeast cytochrome c oxidase have been characterized. Genes represented by these complementation groups are not required for mitochondrial transcription, transcript processing, or translation per se ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00405753
更新日期:1987-01-01 00:00:00
abstract::The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00434050
更新日期:1985-01-01 00:00:00
abstract::The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326414
更新日期:1992-12-01 00:00:00
abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0642-y
更新日期:2017-05-01 00:00:00
abstract::Cenococcum geophilum is an ecologically important mycorrhizal fungus with a global distribution and a wide host range. It has been difficult to study since it forms only sterile mycelia and, occasionally, sclerotial bodies. Because of its lack of morphological variability, its taxonomy and phylogenetic origins have un...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050449
更新日期:1999-06-01 00:00:00
abstract::To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050494
更新日期:1999-10-01 00:00:00
abstract::Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050332
更新日期:1998-04-01 00:00:00
abstract::The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0445-9
更新日期:2003-12-01 00:00:00
abstract::The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0097-7
更新日期:2006-12-01 00:00:00