The primary structure of the leu1+ gene of Schizosaccharomyces pombe.

abstract：:NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...

journal_title：Current genetics

authors： Haas H,Marzluf GA

更新日期：1995-07-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

journal_title：Current genetics

authors： Kapoor S,Maheshwari SC,Tyagi AK

更新日期：1994-04-01 00:00:00

abstract：:Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...

journal_title：Current genetics

authors： Zhou J,Zheng XZ,Lan L,Lin CZ,Wu YB,Lin XJ,Ebbole D,Lu GD,Wang ZH

更新日期：2008-04-01 00:00:00

abstract：:Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel ele...

journal_title：Current genetics

authors： Thoraval D,Régnacq M,Neuville P,Boucherie H

更新日期：1990-11-01 00:00:00

abstract：:In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...

journal_title：Current genetics

authors： Grimm C,Böhl L,Osiewacz HD

更新日期：2015-02-01 00:00:00

abstract：:Mitotic homologous recombination (HR) is an important mechanism for the repair of double-strand breakS and errors occurring during DNA replication. It is likely that the recombinational repair of DNA lesions occurs preferentially by sister chromatid exchanges that have no genetic consequences. However, most geneticall...

journal_title：Current genetics

authors： Prado F,Cortés-Ledesma F,Huertas P,Aguilera A

更新日期：2003-01-01 00:00:00

abstract：:Cells of all living organisms contain complex signal transduction networks to ensure that a wide range of physiological properties are properly adapted to the environmental conditions. The fundamental concepts and individual building blocks of these signalling networks are generally well-conserved from yeast to man; y...

journal_title：Current genetics

authors： Smets B,Ghillebert R,De Snijder P,Binda M,Swinnen E,De Virgilio C,Winderickx J

更新日期：2010-02-01 00:00:00

abstract：:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp ...

journal_title：Current genetics

authors： Houlne G,Schantz R

更新日期：1987-01-01 00:00:00

abstract：:The microtubule-organizing center (MTOC) is the organelle that manages the distribution of microtubule filaments in the cell. It includes such centers of microtubule organization as basal bodies, nucleus-associated bodies, spindle pole bodies and centrosomes. The centrosome is the MTOC in most animal cells. Despite th...

journal_title：Current genetics

authors： Helfant AH

更新日期：2002-02-01 00:00:00

abstract：:The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

journal_title：Current genetics

authors： Blázquez MA,Gancedo C

更新日期：1994-02-01 00:00:00

abstract：:A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

journal_title：Current genetics

authors： Hooley P,Shawcross SG,Strike P

更新日期：1988-11-01 00:00:00

abstract：:Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produ...

journal_title：Current genetics

authors： Whisson SC,Drenth A,Maclean DJ,Irwin JA

更新日期：1994-12-01 00:00:00

abstract：:Studies from this laboratory have suggested that mitochondrial (mt) transcription in yeast (Saccharomyces cerevisiae) is governed by changing cellular cAMP levels, and that the mechanism of such transcriptional regulation requires cAMP-dependent protein kinase (PKA) activity; these observations, in turn, suggest a tra...

journal_title：Current genetics

authors： Iqbal J,Gérard HC,Rahman MU,Hudson AP

更新日期：1996-12-01 00:00:00

abstract：:The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...

journal_title：Current genetics

authors： Yague-Sanz C,Vázquez E,Sánchez M,Antequera F,Hermand D

更新日期：2017-05-01 00:00:00

abstract：:Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...

journal_title：Current genetics

authors： Radzinski M,Reichmann D

更新日期：2019-02-01 00:00:00

abstract：:O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...

journal_title：Current genetics

authors： Zakrzewska A,Migdalski A,Saloheimo M,Penttila ME,Palamarczyk G,Kruszewska JS

更新日期：2003-04-01 00:00:00

abstract：:Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...

journal_title：Current genetics

authors： Rosario-Cruz Z,Boyd JM

更新日期：2016-02-01 00:00:00

abstract：:We have isolated and characterized a Ty element from a yeast cosmid library which exhibits several unusual features: it is flanked by non-homologous delta elements and directly associated with a singular delta element. A tRNA(Glu3) gene and tRNA(Cys) gene are found in conjunction with this element, located in opposite...

journal_title：Current genetics

authors： Stucka R,Hauber J,Feldmann H

更新日期：1986-01-01 00:00:00

abstract：:Extracellular proteins of filamentous fungi are important for biomedical and biotechnological applications. Aspergillus terreus not only comprises an important class of organisms that have significant commercial relevance to the biotechnology industry, but also is an emerging fungal pathogen. However, no information i...

journal_title：Current genetics

authors： Han MJ,Kim NJ,Lee SY,Chang HN

更新日期：2010-08-01 00:00:00

abstract：:The separation and identification of siderophores produced by microorganisms is a time-consuming and an expensive procedure. We have developed a new and efficient method to identify siderophores using well-established Saccharomyces cerevisiae deletion mutants. The Deltafet3,arn strains fail to sustain growth, even whe...

journal_title：Current genetics

authors： Park YS,Kim JH,Chang HI,Kim SW,Paik HD,Kang CW,Kim TH,Sung HC,Yun CW

更新日期：2007-09-01 00:00:00

abstract：:Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...

journal_title：Current genetics

authors： Schweingruber ME,Edenharter E,Zurlinden A,Stockmaier KM

更新日期：1992-10-01 00:00:00

abstract：:The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...

journal_title：Current genetics

authors： Peng G,Xie L,Hu J,Xia Y

更新日期：2009-06-01 00:00:00

abstract：:1. Nuclear mutants lacking cytochrome c l spectrum have been isolated. 2. The electron flow through the respiratory chain of the mutants is defect in the cytochrome bc 1-segment. 3. Complementation analysis has yielded two major complementation groups. 4. Tetrade analysis has established the two complementation groups...

journal_title：Current genetics

authors： Lang BF,Kaudewitz F

更新日期：1982-12-01 00:00:00

abstract：:We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isole...

journal_title：Current genetics

authors： Okada H,Toh-e A

更新日期：1992-02-01 00:00:00

abstract：:RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...

journal_title：Current genetics

authors： Arnau J,Housego AP,Oliver RP

更新日期：1994-05-01 00:00:00

abstract：:The cloning of fga1, the gene encoding a G protein alpha subunit, was performed by standard PCR techniques and by screening a Fusarium oxysporum genomic library, using the PCR product as a probe. The full-length open reading frame spanned 1,059 nucleotides and the deduced primary structure of the protein (353 amino ac...

journal_title：Current genetics

authors： Jain S,Akiyama K,Mae K,Ohguchi T,Takata R

更新日期：2002-09-01 00:00:00

abstract：:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

journal_title：Current genetics

authors： Aleksenko AY

更新日期：1994-10-01 00:00:00

abstract：:The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. C...

journal_title：Current genetics

authors： Katsura K,Suzuki F,Miyashita SI,Nishi T,Hirochika H,Hashiba T

更新日期：1997-12-01 00:00:00

abstract：:Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We he...

journal_title：Current genetics

authors： Tsai YY,Ohashi T,Kanazawa T,Polburee P,Misaki R,Limtong S,Fujiyama K

更新日期：2017-05-01 00:00:00