Targeted disruption of a G protein alpha subunit gene results in reduced pathogenicity in Fusarium oxysporum.

abstract：:Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences...

journal_title：Current genetics

authors： Arnau J,Oliver RP

更新日期：1993-05-01 00:00:00

abstract：:Phosphatidylcholines (PCs) are a class of major cell membrane phospholipids that participate in many physiological processes. Three genes, choA, choB and choC, have been proposed to function in the endogenous biosynthesis of PC in Aspergillus nidulans. In this study, we characterize the choC gene encoding a putative h...

journal_title：Current genetics

authors： Tao L,Gao N,Chen S,Yu JH

更新日期：2010-06-01 00:00:00

abstract：:A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothall...

journal_title：Current genetics

authors： van Zyl WH,Lodolo EJ,Gericke M

更新日期：1993-01-01 00:00:00

abstract：:Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...

journal_title：Current genetics

authors： Goarin A,Silar P,Malagnac F

更新日期：2015-05-01 00:00:00

abstract：:Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...

journal_title：Current genetics

authors： López-Farfán D,Reyes-Darias JA,Krell T

更新日期：2017-06-01 00:00:00

abstract：:When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were...

journal_title：Current genetics

authors： Scouras ZG,Milioni D,Yiangou M,Duchêne M,Domdey H

更新日期：1994-01-01 00:00:00

abstract：:Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc...

journal_title：Current genetics

authors： Pathania A,Liu W,Matityahu A,Irudayaraj J,Onn I

更新日期：2021-01-06 00:00:00

abstract：:The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...

journal_title：Current genetics

authors： Patel D,Evans IH,Bevan EA

更新日期：1990-04-01 00:00:00

abstract：:To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...

journal_title：Current genetics

authors： Iida T,Kobayashi T

更新日期：2019-08-01 00:00:00

abstract：:Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii. These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis ...

journal_title：Current genetics

authors： Nosek J,Filipp D,Bederkova K,Griac P

更新日期：1993-03-01 00:00:00

abstract：:We have isolated suppressors of a PHO4c (a positive regulator) mutant which normally confers weak constitutivity for acid phosphatase production on the Saccharomyces cell. One dominant suppressor (PHO80-2) was found to be an allele of PHO80 (a negative regulator) that changes G to A, resulting in substitution of isole...

journal_title：Current genetics

authors： Okada H,Toh-e A

更新日期：1992-02-01 00:00:00

abstract：:Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are ...

journal_title：Current genetics

authors： Hillmann F,Shekhova E,Kniemeyer O

更新日期：2015-08-01 00:00:00

abstract：:Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...

journal_title：Current genetics

authors： Radzinski M,Reichmann D

更新日期：2019-02-01 00:00:00

abstract：:Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs). The sucrose non-fermenting protein kinase 1 gene (SNF1) is a key component of the glucose de-repression pathway in ye...

journal_title：Current genetics

authors： Islam KT,Bond JP,Fakhoury AM

更新日期：2017-08-01 00:00:00

abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

journal_title：Current genetics

authors： Tutaj H,Pogoda E,Tomala K,Korona R

更新日期：2019-04-01 00:00:00

abstract：:Mono-ubiquitinated histone H2B (H2B-Ub) is important for chromatin regulation of transcription, chromatin assembly, and also influences heterochromatin. In this review, we discuss the effects of H2B-Ub from nucleosome to higher-order chromatin structure. We then assess what is currently known of the role of H2B-Ub in ...

journal_title：Current genetics

authors： Zukowski A,Johnson AM

更新日期：2018-08-01 00:00:00

abstract：:We previously reported the isolation of mutants hypersensitive to the genotoxic agent 4-nitroquinoline-1-oxide, a potent inducer of oxidative stress. One of the mutants was defective in a gene designated yPTPA1, encoding a protein related to the human phosphotyrosyl phosphatase activator hPTPA, which is believed to pl...

journal_title：Current genetics

authors： Douville J,David J,Fortier PK,Ramotar D

更新日期：2004-08-01 00:00:00

abstract：:A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a se...

journal_title：Current genetics

authors： Bonnard G,Weil JH,Steinmetz A

更新日期：1985-01-01 00:00:00

abstract：:The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

journal_title：Current genetics

authors： Kapoor S,Maheshwari SC,Tyagi AK

更新日期：1994-04-01 00:00:00

abstract：:Yeast ornithine transaminase is known to be induced by arginine and ornithine, through the action of regulatory elements common to arginase induction. We show here that it is subject to a second induction circuit, that which is responsible for urea amidolyase and urea permease induction by allophanate and defined by t...

journal_title：Current genetics

authors： Hennaut C

更新日期：1981-09-01 00:00:00

abstract：:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp ...

journal_title：Current genetics

authors： Houlne G,Schantz R

更新日期：1987-01-01 00:00:00

abstract：:Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...

journal_title：Current genetics

authors： Palmer JD,Stein DB

更新日期：1982-08-01 00:00:00

abstract：:Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...

journal_title：Current genetics

authors： Wirsel S,Turgeon BG,Yoder OC

更新日期：1996-02-01 00:00:00

abstract：:Somatic fusion between cells of Chlamydomonas containing complementing cell wall and auxotrophic mutations and bearing chloroplast markers for resistance to antibiotics (streptomycin or spectinomycin) have been performed to analyze the mode of chloroplast gene transmission in the fusion products. Prototrophic colonies...

journal_title：Current genetics

authors： Matagne RF

更新日期：1981-04-01 00:00:00

abstract：:Ziziphus jujuba is an important woody plant with high economic and medicinal value. Here, we analyzed and characterized the complete chloroplast (cp) genome of Z. jujuba, the first member of the Rhamnaceae family for which the chloroplast genome sequence has been reported. We also built a web browser for navigating th...

journal_title：Current genetics

authors： Ma Q,Li S,Bi C,Hao Z,Sun C,Ye N

更新日期：2017-02-01 00:00:00

abstract：:To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...

journal_title：Current genetics

authors： Pöggeler S

更新日期：1999-10-01 00:00:00

abstract：:The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...

journal_title：Current genetics

authors： Egel R

更新日期：1984-04-01 00:00:00

abstract：:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

journal_title：Current genetics

authors： Ruhland A,Brendel M,Haynes RH

更新日期：1986-01-01 00:00:00

abstract：:In the wood destroying fungus Schizophyllum commune, a well known subject for genetic studies, fruit bodies are produced not only in the course of the sexual cycle but also asexually. Sexual fruiting requires the establishment of a dikaryon which is under the control of the incompatibility factors A and B. Asexual fru...

journal_title：Current genetics

authors： Esser K,Saleh F,Meinhardt F

更新日期：1979-12-01 00:00:00

abstract：:A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...

journal_title：Current genetics

authors： Carbone I,Anderson JB,Kohn LM

更新日期：1995-01-01 00:00:00