Abstract:
:Ziziphus jujuba is an important woody plant with high economic and medicinal value. Here, we analyzed and characterized the complete chloroplast (cp) genome of Z. jujuba, the first member of the Rhamnaceae family for which the chloroplast genome sequence has been reported. We also built a web browser for navigating the cp genome of Z. jujuba ( http://bio.njfu.edu.cn/gb2/gbrowse/Ziziphus_jujuba_cp/ ). Sequence analysis showed that this cp genome is 161,466 bp long and has a typical quadripartite structure of large (LSC, 89,120 bp) and small (SSC, 19,348 bp) single-copy regions separated by a pair of inverted repeats (IRs, 26,499 bp). The sequence contained 112 unique genes, including 78 protein-coding genes, 30 transfer RNAs, and four ribosomal RNAs. The genome structure, gene order, GC content, and codon usage are similar to other typical angiosperm cp genomes. A total of 38 tandem repeats, two forward repeats, and three palindromic repeats were detected in the Z. jujuba cp genome. Simple sequence repeat (SSR) analysis revealed that most SSRs were AT-rich. The homopolymer regions in the cp genome of Z. jujuba were verified and manually corrected by Sanger sequencing. One-third of mononucleotide repeats were found to be erroneously sequenced by the 454 pyrosequencing, which resulted in sequences of 1-4 bases shorter than that by the Sanger sequencing. Analyzing the cp genome of Z. jujuba revealed that the IR contraction and expansion events resulted in ycf1 and rps19 pseudogenes. A phylogenetic analysis based on 64 protein-coding genes showed that Z. jujuba was closely related to members of the Elaeagnaceae family, which will be helpful for phylogenetic studies of other Rosales species. The complete cp genome sequence of Z. jujuba will facilitate population, phylogenetic, and cp genetic engineering studies of this economic plant.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Ma Q,Li S,Bi C,Hao Z,Sun C,Ye Ndoi
10.1007/s00294-016-0612-4subject
Has Abstractpub_date
2017-02-01 00:00:00pages
117-129issue
1eissn
0172-8083issn
1432-0983pii
10.1007/s00294-016-0612-4journal_volume
63pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-011-0363-1
更新日期:2012-02-01 00:00:00
abstract::An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221509
更新日期:1996-04-01 00:00:00
abstract::DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00410436
更新日期:1984-09-01 00:00:00
abstract::Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376740
更新日期:1988-09-01 00:00:00
abstract::Phosphatidylcholines (PCs) are a class of major cell membrane phospholipids that participate in many physiological processes. Three genes, choA, choB and choC, have been proposed to function in the endogenous biosynthesis of PC in Aspergillus nidulans. In this study, we characterize the choC gene encoding a putative h...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-010-0300-8
更新日期:2010-06-01 00:00:00
abstract::The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive tempera...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00376778
更新日期:1981-09-01 00:00:00
abstract::Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0456-8
更新日期:2015-05-01 00:00:00
abstract::During evolution, most of the bacterial genes from the ancestral endosymbiotic alpha-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. A crucial evolutionary step was the establishment of macromolecule import systems to allow the come back of proteins and RNAs int...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-008-0223-9
更新日期:2009-02-01 00:00:00
abstract::Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326582
更新日期:1994-12-01 00:00:00
abstract::In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0667-2
更新日期:2017-08-01 00:00:00
abstract::The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336850
更新日期:1992-03-01 00:00:00
abstract::The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365643
更新日期:1983-04-01 00:00:00
abstract::A double-stranded (ds)RNA, designated as M2, is associated with hypovirulence, conversion of the quinic acid pathway from inducible to constitutive and downregulation of the shikimic acid pathway in the Rhizoctonia solani culture Rhs 1A1. In this study, we report that in the virulent, M2-lacking isolate Rhs 1AP, which...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0375-6
更新日期:2003-05-01 00:00:00
abstract::Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00445879
更新日期:1983-09-01 00:00:00
abstract::Studies of motility in Physarum polycephalum have concentrated on the well-defined actomyosin system in plasmodia. It is clear from recent genetic studies in lower eukaryotes that myosin is involved in a number of physiological processes in addition to the contractile functions previously ascribed to the classical typ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309535
更新日期:1994-02-01 00:00:00
abstract::In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00309601
更新日期:1991-05-01 00:00:00
abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351783
更新日期:1994-05-01 00:00:00
abstract::The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-009-0242-1
更新日期:2009-06-01 00:00:00
abstract::As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-003-0408-1
更新日期:2003-11-01 00:00:00
abstract::A phylogenetic investigation of the autosporic chlorophycean alga species Selenastrum capricornutum using the small subunit (SSU) rRNA gene revealed the unusual presence of six group IC1 introns. Previous studies showed that numerous green algal taxa contain group IC1 introns. However, whereas some algal species harbo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0525-5
更新日期:2004-10-01 00:00:00
abstract::In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418411
更新日期:1986-01-01 00:00:00
abstract::Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391801
更新日期:1982-08-01 00:00:00
abstract::The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequenc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050410
更新日期:1998-12-01 00:00:00
abstract::NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00315785
更新日期:1995-07-01 00:00:00
abstract::Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351712
更新日期:1993-12-01 00:00:00
abstract::Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-007-0158-6
更新日期:2007-11-01 00:00:00
abstract::The complete nucleotide sequence of the Morus mongolica chloroplast (cp) genome was reported and characterized in this study. The cp genome is a circular molecule of 158,459 bp containing a pair of 25,678 bp IR regions, separated by small and large single-copy regions of 19,736 and 87,363 bp, respectively. The number ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-015-0507-9
更新日期:2016-02-01 00:00:00
abstract::We have sequenced a region (7,376-bp) of the mitochondrial (mt) DNA (54 kb) of the cellular slime mold, Dictyostelium discoideum. From the DNA and amino-acid sequence comparisons with known sequences, genes for ATPase subunit 9 (ATP9), cytochrome b (CYTB), NADH dehydrogenase subunits 1, 3 and 6 (ND1, ND3 and ND6), sma...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326157
更新日期:1995-02-01 00:00:00
abstract::Stress granules (SGs) are evolutionarily conserved ribonucleoprotein (RNP) structures that form in response to a variety of environmental and cellular cues. The presence of these RNP granules has been linked to a number of human diseases, including neurodegenerative disorders like amyotrophic lateral sclerosis (ALS) a...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0571-9
更新日期:2016-08-01 00:00:00
abstract::A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050175
更新日期:1997-01-01 00:00:00