Isolation and characterisation of the acetyl-CoA carboxylase gene from Aspergillus nidulans.

Abstract:

:The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequence homology to acetyl-CoA carboxylases from other organisms, putative biotin-, ATP-, HCO3-- and acetyl-CoA- binding sites have been assigned. Northern data and ACC enzyme-activity measurements from A. nidulans suggested that expression of accA was higher in media containing nitrate than ammonia as a sole nitrogen source. Deletion of accA in A. nidulans was unsuccessful. The failure of A. nidulans to grow in the presence of the ACC-specific inhibitor, soraphen A, supplemented with C16-18 fatty acids suggested that ACC is an essential enzyme.

journal_name

Curr Genet

journal_title

Current genetics

authors

Morrice J,MacKenzie DA,Parr AJ,Archer DB

doi

10.1007/s002940050410

subject

Has Abstract

pub_date

1998-12-01 00:00:00

pages

379-85

issue

5

eissn

0172-8083

issn

1432-0983

journal_volume

34

pub_type

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