Abstract:
:Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc3 contributes to the activity and identity of its functional domains is not fully understood. Here, we describe an in-frame five-amino acid insertion mutation after glutamic acid 704 (scc3-E704ins) in yeast Scc3, located in the middle of the second armadillo repeat. Mutated cohesin-scc3-E704ins complexes are unable to establish cohesion. Detailed molecular and genetic analyses revealed that the mutated cohesin has reduced affinity to the Scc2 loader. This inhibits its enrichment at centromeres and chromosomal arms. Mutant complexes show a slow diffusion rate in live cells suggesting that they induce a major conformational change in the complex. The analysis of systematic mutations in the insertion region of Scc3 revealed two conserved aspartic acid residues that are essential for the activity. The study offers a better understanding of the contribution of Scc3 to cohesin activity and the mechanism by which cohesin tethers the sister chromatids during the cell cycle.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Pathania A,Liu W,Matityahu A,Irudayaraj J,Onn Idoi
10.1007/s00294-020-01150-3subject
Has Abstractpub_date
2021-01-06 00:00:00eissn
0172-8083issn
1432-0983pii
10.1007/s00294-020-01150-3pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313195
更新日期:1995-12-01 00:00:00
abstract::To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00956-0
更新日期:2019-08-01 00:00:00
abstract::Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326582
更新日期:1994-12-01 00:00:00
abstract::The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050440
更新日期:1999-03-01 00:00:00
abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351689
更新日期:1992-04-01 00:00:00
abstract::The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351854
更新日期:1993-11-01 00:00:00
abstract::Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-007-0155-9
更新日期:2008-01-01 00:00:00
abstract::Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construc...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0754-z
更新日期:2018-04-01 00:00:00
abstract::Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00984-w
更新日期:2019-10-01 00:00:00
abstract::Cenococcum geophilum is an ecologically important mycorrhizal fungus with a global distribution and a wide host range. It has been difficult to study since it forms only sterile mycelia and, occasionally, sclerotial bodies. Because of its lack of morphological variability, its taxonomy and phylogenetic origins have un...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050449
更新日期:1999-06-01 00:00:00
abstract::From the first identification of bacteria as a causative agent of disease, researchers have been developing methods and techniques to understand their pathogenic processes. For decades, this work has been limited to looking at a small number of genetically manipulatable isolates in in vitro assays or animal models of ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0576-4
更新日期:2016-08-01 00:00:00
abstract::The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differential...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418404
更新日期:1986-01-01 00:00:00
abstract::Ziziphus jujuba is an important woody plant with high economic and medicinal value. Here, we analyzed and characterized the complete chloroplast (cp) genome of Z. jujuba, the first member of the Rhamnaceae family for which the chloroplast genome sequence has been reported. We also built a web browser for navigating th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0612-4
更新日期:2017-02-01 00:00:00
abstract::The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0042-1
更新日期:2006-02-01 00:00:00
abstract::A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0106-x
更新日期:2007-02-01 00:00:00
abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0073-2
更新日期:2006-07-01 00:00:00
abstract::In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between he...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0227-5
更新日期:2009-02-01 00:00:00
abstract::Seven MboI fragments spanning the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae strain D273-10B were cloned in the BamHI site of the integrative yeast vector YIp5 and the capacity for autonomous replication was subsequently assayed in yeast. The positive correlation found between the ars-like activity...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00368060
更新日期:1987-01-01 00:00:00
abstract::Retrotransposons are activated as organisms age, based on work from several model systems. Since these mobile DNA elements can promote genome instability, this has raised the possibility that they can contribute to loss of cellular function with age. Many questions remain to be addressed about the relationship between...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0538-2
更新日期:2016-05-01 00:00:00
abstract::The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384611
更新日期:1987-01-01 00:00:00
abstract::Killer protein 4 (KP4) is a well studied viral toxin secreted by the maize smut fungus Ustilago maydis that kills sensitive Ustilago strains as well as inhibits Fusarium and plant root growth by inhibiting calcium uptake. Numerous small, cysteine-rich proteins have been shown to play a critical role in fungal-plant-ba...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-010-0326-y
更新日期:2011-02-01 00:00:00
abstract::A homologous transformation system for Aspergillus oryzae is described. The system is based on an A. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrG) and the vector pAO4-2, which contains a functional A. oryzae pyrG gene as selection marker. Transformation of the A. oryzae pyrG mutant with circul...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00391472
更新日期:1989-09-01 00:00:00
abstract::The cellulase gene cel3 has been isolated from Agaricus bisporus and sequenced. The 5'-end of the cel3 transcript was determined by primer extension and S1 nuclease protection. Putative regulatory elements have been identified in the cel3 promoter and 3'-untranslated regions. The cel3 coding region is interrupted by s...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050100
更新日期:1996-06-01 00:00:00
abstract::Spontaneous and double-strand break (DSB)-induced gene conversion in Saccharomyces cerevisiae was assayed using non-tandem chromosomal direct repeat crosses and plasmid x chromosome crosses. Each cross involved identical ura3 alleles marked with phenotypically silent restriction fragment length polymorphic (RFLP) muta...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050396
更新日期:1998-10-01 00:00:00
abstract::The cloning and characterization of the Thcut1 gene, which encodes a cutinase protein of the biocontrol fungus Trichoderma harzianum T34, is reported. Cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. The ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0218-6
更新日期:2008-12-01 00:00:00
abstract::We have determined the size, the restriction map and the gene order of the mitochondrial genome of the yeast Saccharomyces uvarum. Sequence analysis of the mitochondrial COXII gene confirmed the position of this yeast in the Saccharomyces cerevisiae-like group, near Saccharomyces cerevisiae and Saccharomyces douglasii...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050308
更新日期:1998-01-01 00:00:00
abstract::Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312631
更新日期:1993-05-01 00:00:00
abstract::Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351843
更新日期:1993-11-01 00:00:00
abstract::Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02208620
更新日期:1996-03-01 00:00:00
abstract::In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050535
更新日期:2000-05-01 00:00:00