Abstract:
:Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously known protein have been noted. The protein is very hydrophobic and is predicted to have a membraneous localisation. In agreement with this hypothesis, the analysis of membrane proteins from stationary-phase cells showed that a strain carrying this gene on a multicopy vector overproduces a protein of 30 kDa. This protein was recognized by antibodies directed against the N-terminal portion of the gene product. Considering its induction in response to heat shock and the apparent molecular weight of its product, this gene was designated HSP30.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Régnacq M,Boucherie Hdoi
10.1007/BF00312631subject
Has Abstractpub_date
1993-05-01 00:00:00pages
435-42issue
5-6eissn
0172-8083issn
1432-0983journal_volume
23pub_type
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