Abstract：
:We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identified yeast prion determinants. Here, we show that [NSI(+)] enhances nonsense codon read-through and inhibits vegetative growth in S. cerevisiae. Using a large-scale overexpression screen to identify genes that impact the phenotypic effects of [NSI(+)], we found that the SUP35 and SUP45 genes encoding the translation termination factors eRF3 and eRF1, respectively, modulate nonsense suppression in [NSI(+)] strains. The VTS1 gene encodes an NQ-enriched RNA-binding protein that enhances nonsense suppression in [NSI(+)] and [nsi(-)] strains. We demonstrate that VTS1 overexpression, like [NSI(+)] induction, causes translational read-through and growth defects in S. cerevisiae.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Nizhnikov AA,Magomedova ZM,Rubel AA,Kondrashkina AM,Inge-Vechtomov SG,Galkin APdoi
10.1007/s00294-011-0363-1subject
Has Abstractpub_date
2012-02-01 00:00:00pages
35-47issue
1eissn
0172-8083issn
1432-0983journal_volume
58pub_type
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