Optional elements in the chloroplast DNAs of chlamydomonas eugametos and C. moewusii: unidirectional gene conversion and co-conversion of adjacent markers in high-viability crossses.

abstract：:Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotrans...

journal_title：Current genetics

authors： Huang KX,Iwakami N,Fujii I,Ebizuka Y,Sankawa U

更新日期：1995-11-01 00:00:00

abstract：:Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

journal_title：Current genetics

authors： Jakobsen KP,Andersen AH,Bjergbæk L

更新日期：2019-10-01 00:00:00

abstract：:This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...

journal_title：Current genetics

authors： Cantatore P,Roberti M,Loguercio Polosa P,Mustich A,Gadaleta MN

更新日期：1990-03-01 00:00:00

abstract：:Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 backgr...

journal_title：Current genetics

authors： King L,Butler G

更新日期：1998-09-01 00:00:00

abstract：:The gene for subunit II of cytochrome oxidase in the yeast Hansenula saturnus was previously shown to be located on a 1.7 kb HindIII-BamHI fragment of mitochondrial DNA (Lawson and Deters, accompanying paper). In this paper, we report the nucleotide sequence of a large part of this fragment, covering the coding region...

journal_title：Current genetics

authors： Lawson JE,Deters DW

更新日期：1985-01-01 00:00:00

abstract：:The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...

journal_title：Current genetics

authors： Scherer S,Lechner S,Böger P

更新日期：1993-11-01 00:00:00

abstract：:nam3 and R705, yeast nuclear omnipotent suppressors of mitochondrial mit- mutations, reverse the superimposed spectrum of trans-recessive splicing defects by affecting the protein composition of the small mitoribosomal subunit. Analysis of the suppressor's interaction suggests that suppression results from mutations i...

journal_title：Current genetics

authors： Boguta M,Mieszczak M,Zagórski W

更新日期：1988-02-01 00:00:00

abstract：:Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define thre...

journal_title：Current genetics

authors： Benko Z,Miklos I,Carr AM,Sipiczki M

更新日期：1997-06-01 00:00:00

abstract：:The regulation of transcription of the class II yeast transposon Ty 1-17 has been examined using a Ty::LEU2 fusion gene. Deletion analysis has defined a 251 bp region within the coding sequence of the TY A gene which is essential for Ty transcription and is also required for diploid control of Ty expression. This down...

journal_title：Current genetics

authors： Warmington JR,Oliver SG

更新日期：1988-05-01 00:00:00

abstract：:A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...

journal_title：Current genetics

authors： Suyama Y,Fukuhara H,Sor F

更新日期：1985-01-01 00:00:00

abstract：:A transformation system utilizing Agrobacterium tumefaciens was developed for targeted gene disruption in Monilinia fructicola, a fungal pathogen that causes brown rot disease of stone fruits. Transformation with a vector containing the neomycin phosphotransferase II (nptII) cassette flanked with 4 kb cutinase gene (M...

journal_title：Current genetics

authors： Lee MH,Bostock RM

更新日期：2006-05-01 00:00:00

abstract：:The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...

journal_title：Current genetics

authors： Janitor M,Subík J

更新日期：1993-10-01 00:00:00

abstract：:The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...

journal_title：Current genetics

authors： Foster SJ,Fitt BD

更新日期：2003-12-01 00:00:00

abstract：:In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...

journal_title：Current genetics

authors： Grimm C,Böhl L,Osiewacz HD

更新日期：2015-02-01 00:00:00

abstract：:Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by alig...

journal_title：Current genetics

authors： Labriola J,Weiss I,Zapatero J,Suyama Y

更新日期：1987-01-01 00:00:00

abstract：:The RAD50 gene in yeast is required for recombination-repair (i.e., the double strand break repair pathway) in mitosis, and for meiotic recombination and sporulation. Both of these processes are complex and seem likely to require a relatively large number of gene products. In order to help define other genes required ...

journal_title：Current genetics

authors： Malone RE,Jordan K,Wardman W

更新日期：1985-01-01 00:00:00

abstract：:Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...

journal_title：Current genetics

authors： Régnacq M,Boucherie H

更新日期：1993-05-01 00:00:00

abstract：:Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...

journal_title：Current genetics

authors： Huynh AD,Leblon G,Zickler D

更新日期：1986-01-01 00:00:00

abstract：:Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...

journal_title：Current genetics

authors： Goarin A,Silar P,Malagnac F

更新日期：2015-05-01 00:00:00

abstract：:Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...

journal_title：Current genetics

authors： Wirsel S,Turgeon BG,Yoder OC

更新日期：1996-02-01 00:00:00

abstract：:Timely progression of living cells through the cell cycle is precisely regulated. This involves a series of phosphorylation events which are regulated by various cyclins, activated in coordination with the cell cycle progression. Phosphorylated proteins govern cell growth, division as well as duplication of the geneti...

journal_title：Current genetics

authors： Dmowski M,Fijałkowska IJ

更新日期：2017-12-01 00:00:00

abstract：:Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...

journal_title：Current genetics

authors： Haase E,Servos J,Brendel M

更新日期：1992-04-01 00:00:00

abstract：:Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...

journal_title：Current genetics

authors： Kupper U,Linden M,Cao KZ,Lerch K

更新日期：1990-11-01 00:00:00

abstract：:As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...

journal_title：Current genetics

authors： Cahoon AB,Cunningham KA,Bollenbach TJ,Stern DB

更新日期：2003-11-01 00:00:00

abstract：:The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...

journal_title：Current genetics

authors： Peng G,Xie L,Hu J,Xia Y

更新日期：2009-06-01 00:00:00

abstract：:Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...

journal_title：Current genetics

authors： Harrington TC,McNew DL

更新日期：1997-07-01 00:00:00

abstract：:A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...

journal_title：Current genetics

authors： Haase E,Brendel M

更新日期：1990-10-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in an Escherichia coli-Schizosaccharomyces pombe (E. coli-S. pombe) replicative plasmid to a 1.76 kb HindIII fragment. This plasmid is maintained at a high co...

journal_title：Current genetics

authors： Bach ML

更新日期：1987-01-01 00:00:00

abstract：:The sequence of a representative mitochondrial gene COXI, encoding cytochrome c oxidase subunit I, was determined in five species that cover all the orders of the Prymnesiophyta with the exception of the Pavlovales. Through this analysis, we noticed that the 'stop' codon UGA appears frequently and, specifically, at co...

journal_title：Current genetics

authors： Hayashi-Ishimaru Y,Ehara M,Inagaki Y,Ohama T

更新日期：1997-10-01 00:00:00