Abstract:
:Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotransferase gene under the control of Aspergillus nidulans sequences, were used respectively. Transformation frequencies with these plasmids were 10-20 transformants per micrograms of DNA per 4-8 x 10(7) viable protoplasts. Integration of plasmid DNAs into chromosomal DNAs was confirmed by Southern-blot analysis. Copy numbers and sites of integration varied among transformants. The integrated plasmid DNAs conferring a drug-resistant phenotype were mitotically stable with or without selection. The demonstration of such transformation systems in the essential first step in the application of recombinant DNA technology to study the biosynthetic genes of anthraquinone and related compounds in P. islandicum and P. frequentans.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Huang KX,Iwakami N,Fujii I,Ebizuka Y,Sankawa Udoi
10.1007/BF00518172subject
Has Abstractpub_date
1995-11-01 00:00:00pages
580-4issue
6eissn
0172-8083issn
1432-0983journal_volume
28pub_type
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