Transformations of Penicillium islandicum and Penicillium frequentans that produce anthraquinone-related compounds.

Abstract:

:Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotransferase gene under the control of Aspergillus nidulans sequences, were used respectively. Transformation frequencies with these plasmids were 10-20 transformants per micrograms of DNA per 4-8 x 10(7) viable protoplasts. Integration of plasmid DNAs into chromosomal DNAs was confirmed by Southern-blot analysis. Copy numbers and sites of integration varied among transformants. The integrated plasmid DNAs conferring a drug-resistant phenotype were mitotically stable with or without selection. The demonstration of such transformation systems in the essential first step in the application of recombinant DNA technology to study the biosynthetic genes of anthraquinone and related compounds in P. islandicum and P. frequentans.

journal_name

Curr Genet

journal_title

Current genetics

authors

Huang KX,Iwakami N,Fujii I,Ebizuka Y,Sankawa U

doi

10.1007/BF00518172

subject

Has Abstract

pub_date

1995-11-01 00:00:00

pages

580-4

issue

6

eissn

0172-8083

issn

1432-0983

journal_volume

28

pub_type

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