Abstract:
:Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNNG, to 4-NQO and triaziquone, and to mutagens 4-NQO, MNNG, and triaziquone, respectively. Integration of the gene disruption-constructs into the haploid yeast genome yielded viable null-mutants with a mutagen-sensitive phenotype. Thus, copy number of these non-essential yeast genes determines the relative resistance to certain chemical mutagens, with zero copies yielding a phenotype of mutagen sensitivity and multiple copies one of mutagen hyper-resistance, respectively.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Haase E,Servos J,Brendel Mdoi
10.1007/BF00351689subject
Has Abstractpub_date
1992-04-01 00:00:00pages
319-24issue
4-5eissn
0172-8083issn
1432-0983journal_volume
21pub_type
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