Unexpectedly long 14S ribosomal RNA gene in Tetrahymena mitochondria.


:Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by aligning our sequence with that of the corresponding small rRNA from E. coli, find that the 14S rDNA is at least 1,635 bases in length. We propose, based on the results of hybridization studies, that this unexpected length is due to the presence of 7S RNA sequence within the 14S gene sequence. The 7S region is apparently lost from the 14S rRNA, yet is still a component of the ribosome.


Curr Genet


Current genetics


Labriola J,Weiss I,Zapatero J,Suyama Y




Has Abstract


1987-01-01 00:00:00












  • A mutated swi4 gene causes duplications in the mating-type region of Schizosaccharomyces pombe.

    abstract::Efficient mating-type (MT) switching in homothallic strains of Schizosaccharomyces pombe is significantly reduced if they have a mutation in any of the eleven known swi genes. The swi4 mutation causes heterothallic as well as homothallic segregants, both of which have duplications in the MT region. In contrast to homo...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Fleck O,Heim L,Gutz H

    更新日期:1990-12-01 00:00:00

  • Heterologous expression of a pleiotropic drug resistance transporter from Phytophthora sojae in yeast transporter mutants.

    abstract::A system for the expression of an ATP binding cassette (ABC) transporter from the soybean pathogen Phytophthora sojae is described. Pdr1, an ABC transporter with homology to the pleiotropic drug resistance (PDR) family of transporters, was cloned by primer walking from a P. sojae genomic library. Reverse transcriptase...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Connolly MS,Sakihama Y,Phuntumart V,Jiang Y,Warren F,Mourant L,Morris PF

    更新日期:2005-12-01 00:00:00

  • Temperature sensitivity of RNA editing and intron splicing reactions in the plastid ndhB transcript.

    abstract::Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Karcher D,Bock R

    更新日期:2002-04-01 00:00:00

  • Isolation and characterisation of the mating-type (MAT) locus from Rhynchosporium secalis.

    abstract::The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Foster SJ,Fitt BD

    更新日期:2003-12-01 00:00:00

  • The use of RAPD markers in the genetic analysis of the plant pathogenic fungus Cladosporium fulvum.

    abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Arnau J,Housego AP,Oliver RP

    更新日期:1994-05-01 00:00:00

  • Complete mitochondrial genomes of two cockroaches, Blattella germanica and Periplaneta americana, and the phylogenetic position of termites.

    abstract::The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequen...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Xiao B,Chen AH,Zhang YY,Jiang GF,Hu CC,Zhu CD

    更新日期:2012-04-01 00:00:00

  • Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products: multimerization, protein priming, and nucleotide preference.

    abstract::Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Yushenova IA,Arkhipova IR

    更新日期:2018-12-01 00:00:00

  • Deletion of the Cochliobolus heterostrophus mating-type (MAT) locus promotes the function of MAT transgenes.

    abstract::Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Wirsel S,Turgeon BG,Yoder OC

    更新日期:1996-02-01 00:00:00

  • Transcript mapping and processing of mitochondrial RNA in Dictyostelium discoideum.

    abstract::The circular mitochondrial genome of Dictyostelium discoideum has a size of 55,564 base pairs. We present here a complete and detailed transcription map of the mitochondrial DNA. Eight major, polycistronic transcripts encoding polypeptides, ribosomal RNAs and interspersed transfer RNAs were identified in Northern hybr...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Barth C,Greferath U,Kotsifas M,Tanaka Y,Alexander S,Alexander H,Fisher PR

    更新日期:2001-07-01 00:00:00

  • The expression of many chemoreceptor genes depends on the cognate chemoeffector as well as on the growth medium and phase.

    abstract::Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: López-Farfán D,Reyes-Darias JA,Krell T

    更新日期:2017-06-01 00:00:00

  • Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger.

    abstract::Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ngiam C,Jeenes DJ,Archer DB

    更新日期:1997-02-01 00:00:00

  • Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth.

    abstract::The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is cl...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Richman TJ,Toenjes KA,Morales SE,Cole KC,Wasserman BT,Taylor CM,Koster JA,Whelihan MF,Johnson DI

    更新日期:2004-06-01 00:00:00

  • Comparative analysis of the region of the mitochondrial genome containing the ATPase subunit 9 gene in the two related yeast species Saccharomyces douglasii and Saccharomyces cerevisiae.

    abstract::We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Nicoletti L,Laveder P,Pellizzari R,Cardazzo B,Carignani G

    更新日期:1994-06-01 00:00:00

  • Expression of tyrosinase in vegetative cultures of Neurospora crassa transformed with a metallothionein promoter/protyrosinase fusion gene.

    abstract::Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kupper U,Linden M,Cao KZ,Lerch K

    更新日期:1990-11-01 00:00:00

  • Agrobacterium-tumefaciens-mediated transformation of antifungal-lipopeptide-producing fungus Coleophoma empetri F-11899.

    abstract::The filamentous fungus Coleophoma empetri F-11899 produces an echinocandin-like compound FR901379, the original source for micafungin which is prescribed to treat deep-seated mycoses. Despite its industrial importance, no genetic information on C. empetri F-11899 is currently available. To characterize FR901379 biosyn...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Yamada M,Yawata K,Orino Y,Ueda S,Isogai Y,Taguchi G,Shimosaka M,Hashimoto S

    更新日期:2009-12-01 00:00:00

  • Variety is the spice of life: how to explore a redox-dependent heterogeneity in genomically identical cellular populations.

    abstract::Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Radzinski M,Reichmann D

    更新日期:2019-02-01 00:00:00

  • Ace2p, a regulator of CTS1 (chitinase) expression, affects pseudohyphal production in Saccharomyces cerevisiae.

    abstract::Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 backgr...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: King L,Butler G

    更新日期:1998-09-01 00:00:00

  • Conversion of homothallic yeast to heterothallism through HO gene disruption.

    abstract::A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothall...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: van Zyl WH,Lodolo EJ,Gericke M

    更新日期:1993-01-01 00:00:00

  • Rubisco spacer sequence divergence in the rhodophyte alga Gracilaria verrucosa and closely related species.

    abstract::In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Destombe C,Douglas SE

    更新日期:1991-05-01 00:00:00

  • Cloning and characterization of the Thcut1 gene encoding a cutinase of Trichoderma harzianum T34.

    abstract::The cloning and characterization of the Thcut1 gene, which encodes a cutinase protein of the biocontrol fungus Trichoderma harzianum T34, is reported. Cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. The ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Rubio MB,Cardoza RE,Hermosa R,Gutiérrez S,Monte E

    更新日期:2008-12-01 00:00:00

  • Cloning, characterisation and regulation of the ornithine transaminase (otaA) gene of Aspergillus nidulans.

    abstract::The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Dzikowska A,Swianiewicz M,Talarczyk A,Wisniewska M,Goras M,Scazzocchio C,Weglenski P

    更新日期:1999-03-01 00:00:00

  • Inheritance of mitochondrial DNA in sexual crosses and protoplast cell fusions in Lentinula edodes.

    abstract::By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Fukuda M,Harada Y,Imahori S,Fukumasa-Nakai Y,Hayashi Y

    更新日期:1995-05-01 00:00:00

  • Characterization and prevalence of a circular mitochondrial plasmid in senescence-prone isolates of Neurospora intermedia.

    abstract::Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: D'Souza AD,Sultana S,Maheshwari R

    更新日期:2005-03-01 00:00:00

  • Delta-9 fatty acid desaturase gene containing a carboxyl-terminal cytochrome b5 domain from the red alga Cyanidioschyzon merolae.

    abstract::A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acid...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Itoh R,Toda K,Takahashi H,Takano H,Kuroiwa T

    更新日期:1998-03-01 00:00:00

  • Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.

    abstract::Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Aleksenko AY

    更新日期:1994-10-01 00:00:00

  • In vitro mutagenesis of the mitochondrial leucyl-tRNA synthetase of S. cerevisiae reveals residues critical for its in vivo activities.

    abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Li GY,Herbert CJ,Labouesse M,Slonimski PP

    更新日期:1992-07-01 00:00:00

  • Double-stranded RNA in the entomopathogenic fungus metarhizium flavoviride.

    abstract::Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Martins MK,Furlaneto MC,Sosa-Gomez DR,Faria MR,Fungaro MH

    更新日期:1999-08-01 00:00:00

  • Mapping and characterization of Paracentrotus lividus mitochondrial transcripts: multiple and overlapping transcription units.

    abstract::This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Cantatore P,Roberti M,Loguercio Polosa P,Mustich A,Gadaleta MN

    更新日期:1990-03-01 00:00:00

  • The beta-tubulin genes of Drosophila auraria are arranged in a cluster.

    abstract::When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Scouras ZG,Milioni D,Yiangou M,Duchêne M,Domdey H

    更新日期:1994-01-01 00:00:00

  • Biochemical and molecular characterization of a putative endoglucanase in Magnaporthe grisea.

    abstract::Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Zhou J,Zheng XZ,Lan L,Lin CZ,Wu YB,Lin XJ,Ebbole D,Lu GD,Wang ZH

    更新日期:2008-04-01 00:00:00