Genetic variability and relatedness in the complex group of black Aspergilli based on random amplification of polymorphic DNA.

Abstract:

:A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most commonly used, namely restriction fragment length polymorphisms on rDNA and isozyme analysis. The eight main groups as assigned by RFLP were also distinguished by RAPD patterns. On the basis of 122 polymorphic RAPD products using six random primers, the 17 collection strains examined could be subdivided into 15 distinct sub-groups. We suggest that the RAPD method is a quick and reliable tool for establishing the amount of genetic variability in closely related species. Our study indicates that the complex group of black Aspergilli is characterized by a high degree of genetic differentiation. This is also apparent from the considerable karyotype variation present in the group.

journal_name

Curr Genet

journal_title

Current genetics

authors

Megnegneau B,Debets F,Hoekstra RF

doi

10.1007/BF00310893

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

323-9

issue

4

eissn

0172-8083

issn

1432-0983

journal_volume

23

pub_type

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