Abstract：
:Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous Cdc25 mitotic inducer for vegetative growth and (b). that CDC25A is able to partially restore a functional checkpoint in response to both ionising and UV irradiation, but not a DNA replication checkpoint. We then describe a simple assay in which we demonstrate that growth of the humanised CDC25A strain is strongly repressed in a CDC25-dependent manner by BN2003, a potent chemical inhibitor of CDC25 belonging to the benzothiazoledione family. The ease of manipulation of fission yeast humanised CDC25 cells and the simplicity of the above assay offer a powerful tool with which to investigate the specificity of pharmacological inhibitors of CDC25.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Mondesert O,Lemaire M,Brezak MC,Galera-Contour MO,Prevost G,Ducommun B,Bugler Bdoi
10.1007/s00294-003-0483-3subject
Has Abstractpub_date
2004-05-01 00:00:00pages
283-8issue
5eissn
0172-8083issn
1432-0983journal_volume
45pub_type
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