Abstract:
:In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (functional) map, 70% of the chondriome was cloned in E. coli vectors. Using mitochondrial genes from Saccharomyces cerevisiae, six structural genes were located on the Podospora chondriome by cross hybridization experiments. There is strong evidence that the plasmid is inserted into the cytochrome b gene. A comparison of the genetic map of the Podospora chondriome with those of Neurospora crassa and Aspergillus nidulans exhibits a rather good accordance with respect to the sequence of genes.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Kück U,Esser Kdoi
10.1007/BF00365705subject
Has Abstractpub_date
1982-07-01 00:00:00pages
143-7issue
2eissn
0172-8083issn
1432-0983journal_volume
5pub_type
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