Genetic map of mitochondrial DNA in Podospora anserina.

Abstract:

:In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (functional) map, 70% of the chondriome was cloned in E. coli vectors. Using mitochondrial genes from Saccharomyces cerevisiae, six structural genes were located on the Podospora chondriome by cross hybridization experiments. There is strong evidence that the plasmid is inserted into the cytochrome b gene. A comparison of the genetic map of the Podospora chondriome with those of Neurospora crassa and Aspergillus nidulans exhibits a rather good accordance with respect to the sequence of genes.

journal_name

Curr Genet

journal_title

Current genetics

authors

Kück U,Esser K

doi

10.1007/BF00365705

subject

Has Abstract

pub_date

1982-07-01 00:00:00

pages

143-7

issue

2

eissn

0172-8083

issn

1432-0983

journal_volume

5

pub_type

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