Abstract:
:The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a potential glycosylation site. Two non-secreting S. cerevisiae strains (FY1679 and var. uvarum) had non-transcribed copies of functional genes. Maximum enzyme activity was achieved with the S. cerevisiae FY1679 gene in an expressing vector, with an enzyme activity of 51 micromol of reducing sugar released from polygalacturonic acid microg protein(-1) min(-1), the highest so far reported for a yeast.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Jia J,Wheals Adoi
10.1007/s002940000160subject
Has Abstractpub_date
2000-12-01 00:00:00pages
264-70issue
5eissn
0172-8083issn
1432-0983journal_volume
38pub_type
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