An approach to yeast classification by mapping mitochondrial DNA from Dekkera/Brettanomyces and Eeniella genera.

abstract：:The recovery of spontaneous canavanine-resistant mutants is reduced dramatically in a strain of Saccharomyces cerevisiae that carries a suppressed can 1-100 allele and is permeable to and auxotrophic for thymidylate. This effect does not occur in an isogenic strain that neither takes up nor requires the nucleotide. Ho...

journal_title：Current genetics

authors： Kohalmi SE,Kunz BA

更新日期：1989-02-01 00:00:00

abstract：:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...

journal_title：Current genetics

authors： Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina L

更新日期：1993-01-01 00:00:00

abstract：:Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...

journal_title：Current genetics

authors： Schaaff-Gerstenschläger I,Zimmermann FK

更新日期：1993-11-01 00:00:00

abstract：:Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...

journal_title：Current genetics

authors： Wirsel S,Turgeon BG,Yoder OC

更新日期：1996-02-01 00:00:00

abstract：:Some diploid strains of Saccharomyces cerevisiae can grow both as a spherical yeast form and as a filamentous pseudohyphal form. Most yeasts capable of forming pseudohyphae possess a functional FLO8 gene. We show that disrupting the ACE2 transcription factor results in the production of pseudohyphae in a flo8-1 backgr...

journal_title：Current genetics

authors： King L,Butler G

更新日期：1998-09-01 00:00:00

abstract：:Saccharomyces cerevisiae adapts to oxidative, osmotic stress and nutrient deprivation through transcriptional changes, decreased proliferation, and entry into other developmental pathways such as pseudohyphal formation and sporulation. Inositol pyrophosphates are necessary for these cellular responses. Inositol pyroph...

journal_title：Current genetics

authors： Morrissette VA,Rolfes RJ

更新日期：2020-10-01 00:00:00

abstract：:We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both statio...

journal_title：Current genetics

authors： Polakowska R,Perozzi G,Prakash L

更新日期：1986-01-01 00:00:00

abstract：:Most organisms prefer to utilize glucose as a carbon source. Accordingly, the expression of genes involved in the catabolism of other carbon sources is repressed by the presence of glucose in a process known as (carbon) catabolite repression. However, much less is known about the relationships between "poor" carbon so...

journal_title：Current genetics

authors： Simpson-Lavy K,Kupiec M

更新日期：2019-12-01 00:00:00

abstract：:Killer protein 4 (KP4) is a well studied viral toxin secreted by the maize smut fungus Ustilago maydis that kills sensitive Ustilago strains as well as inhibits Fusarium and plant root growth by inhibiting calcium uptake. Numerous small, cysteine-rich proteins have been shown to play a critical role in fungal-plant-ba...

journal_title：Current genetics

authors： Brown DW

更新日期：2011-02-01 00:00:00

abstract：:Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

journal_title：Current genetics

authors： Jakobsen KP,Andersen AH,Bjergbæk L

更新日期：2019-10-01 00:00:00

abstract：:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...

journal_title：Current genetics

authors： Viterbo A,Montero M,Ramot O,Friesem D,Monte E,Llobell A,Chet I

更新日期：2002-11-01 00:00:00

abstract：:In previous work, four genes involved in mating-type determination were cloned from reference strains of Pyrenopeziza brassicae; three genes, PAD1, PMT1, and PHB1 (re-named henceforth as MAT-1-1, MAT-1-4, and MAT1-3, respectively), are encoded by the MAT-1 idiomorph, and one gene, PHB2 (re-named MAT-2), by the corresp...

journal_title：Current genetics

authors： Singh G,Dyer PS,Ashby AM

更新日期：1999-11-01 00:00:00

abstract：:Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...

journal_title：Current genetics

authors： Hilber UW,Bodmer M,Smith FD,Köller W

更新日期：1994-02-01 00:00:00

abstract：:Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...

journal_title：Current genetics

authors： Leroy P,Bazetoux S,Quétier F,Delbut J,Bervillé A

更新日期：1985-03-01 00:00:00

abstract：:The filamentous fungus Coleophoma empetri F-11899 produces an echinocandin-like compound FR901379, the original source for micafungin which is prescribed to treat deep-seated mycoses. Despite its industrial importance, no genetic information on C. empetri F-11899 is currently available. To characterize FR901379 biosyn...

journal_title：Current genetics

authors： Yamada M,Yawata K,Orino Y,Ueda S,Isogai Y,Taguchi G,Shimosaka M,Hashimoto S

更新日期：2009-12-01 00:00:00

abstract：:The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...

journal_title：Current genetics

authors： Zheng D,Nielsen BL,Daniell H

更新日期：1997-08-01 00:00:00

abstract：:Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...

journal_title：Current genetics

authors： Goarin A,Silar P,Malagnac F

更新日期：2015-05-01 00:00:00

abstract：:The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

journal_title：Current genetics

authors： Howe CJ

更新日期：1985-01-01 00:00:00

abstract：:The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

journal_title：Current genetics

authors： Dzikowska A,Swianiewicz M,Talarczyk A,Wisniewska M,Goras M,Scazzocchio C,Weglenski P

更新日期：1999-03-01 00:00:00

abstract：:The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...

journal_title：Current genetics

authors： Madsen LH,Kreiberg JD,Gausing K

更新日期：1991-05-01 00:00:00

abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

journal_title：Current genetics

authors： Tutaj H,Pogoda E,Tomala K,Korona R

更新日期：2019-04-01 00:00:00

abstract：:For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...

journal_title：Current genetics

authors： da Silva Ferreira ME,Malavazi I,Savoldi M,Brakhage AA,Goldman MH,Kim HS,Nierman WC,Goldman GH

更新日期：2006-07-01 00:00:00

abstract：:We have cloned and sequenced human and bovine cDNAs for the beta subunit of the ATP synthase (ATP-syn beta), a nuclear DNA (nDNA) encoded oxidative phosphorylation (OXPHOS) gene. The two cDNAs were found to share 99% amino acid homology and 94% nucleotide homology. The evolutionary rate of ATPsyn beta was then compare...

journal_title：Current genetics

authors： Wallace DC,Ye JH,Neckelmann SN,Singh G,Webster KA,Greenberg BD

更新日期：1987-01-01 00:00:00

abstract：:Cohesin is one of three multi-protein structural maintenance of chromosome (SMC) complexes that regulate eukaryotic chromosome dynamics. It forms a ring-shaped structure that embraces sister chromatids through interphase to promote their pairing. In preparation for mitosis, most cohesin is stripped from the chromosome...

journal_title：Current genetics

authors： Lin SJ,O'Connell MJ

更新日期：2017-10-01 00:00:00

abstract：:Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

journal_title：Current genetics

authors： D'Souza AD,Sultana S,Maheshwari R

更新日期：2005-03-01 00:00:00

abstract：:Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...

journal_title：Current genetics

authors： Hasunuma K,Shinohara Y

更新日期：1985-01-01 00:00:00

abstract：:Mycorrhizal ascomycetes are ecologically and commercially important fungi that have proved impervious to genetic transformation so far. We report here on the successful transient transformation of Tuber borchii, an ectomycorrhizal ascomycete that colonizes a variety of trees and produces highly prized hypogeous fruitb...

journal_title：Current genetics

authors： Grimaldi B,de Raaf MA,Filetici P,Ottonello S,Ballario P

更新日期：2005-07-01 00:00:00

abstract：:The ribosomal DNA repeat unit of Sphaeronaemella fimicola was found to be a 13.7-kb tandem repeat with a relatively long nontranscribed spacer (NTS) and an unusually compact ribosomal RNA gene cluster. The DNA sequence of an 850-bp PCR amplification product containing the 3' end of the small subunit rRNA (SSrRNA) gene...

journal_title：Current genetics

authors： Hausner G,Klassen GR,Reid J

更新日期：1993-01-01 00:00:00

abstract：:Microbial pathogens secrete an array of cell wall-degrading enzymes to break down the structure of the host cell wall to facilitate colonization of the host tissue. To better understand their role in the pathogenesis, a putative endoglucanase from Magnaporthe grisea was characterized in this paper. SignalP-3.0 analysi...

journal_title：Current genetics

authors： Zhou J,Zheng XZ,Lan L,Lin CZ,Wu YB,Lin XJ,Ebbole D,Lu GD,Wang ZH

更新日期：2008-04-01 00:00:00

abstract：:Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are ...

journal_title：Current genetics

authors： Hillmann F,Shekhova E,Kniemeyer O

更新日期：2015-08-01 00:00:00