Cloning and characterization of the Thcut1 gene encoding a cutinase of Trichoderma harzianum T34.


:The cloning and characterization of the Thcut1 gene, which encodes a cutinase protein of the biocontrol fungus Trichoderma harzianum T34, is reported. Cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. The Thcut1 gene was isolated by screening of a genomic DNA library from EST 2104, generated from a T. harzianum T34 cDNA library constructed under mycoparasitic and nutrient stress conditions, as a probe. Thcut1 shows similarity with fungal cutinase genes and is present as a single copy in the genome of T. harzianum. RNA blot analyses revealed that Thcut1 mRNA is strongly induced in vitro by olive oil and the cutin monomer 16-hydroxy-hexadecanoic acid and that it is repressed by glucose. Significant transcript levels were also detected when strawberry plants or pectin were present in the media and in the absence of glucose. Expression of the Thcut1 gene in Pichia pastoris gave rise to transformants with high esterase activity and a high level of secretion of the THCUT1 protein. Recombinant cutinase secretion at flask level indicated that P. pastoris transformants could be applied to set up the production of this enzyme at industrial scale.


Curr Genet


Current genetics


Rubio MB,Cardoza RE,Hermosa R,Gutiérrez S,Monte E




Has Abstract


2008-12-01 00:00:00












  • An analysis of the sequence of part of the right arm of chromosome II of S. cerevisiae reveals new genes encoding an amino-acid permease and a carboxypeptidase.

    abstract::We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted mem...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Nasr F,Bécam AM,Grzybowska E,Zagulski M,Slonimski PP,Herbert CJ

    更新日期:1994-07-01 00:00:00

  • Identification of extragenic suppressors of the cif1 mutation in Saccharomyces cerevisiae.

    abstract::The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Blázquez MA,Gancedo C

    更新日期:1994-02-01 00:00:00

  • Sgs1 helicase is required for efficient PCNA monoubiquitination and translesion DNA synthesis in Saccharomyces cerevisiae.

    abstract::DNA-damage tolerance (DDT) is employed by eukaryotes to deal with replication blocks on the template strand, and is divided into two parallel pathways that are activated by sequential ubiquitination of proliferating cell nuclear antigen (PCNA) at the Lys164 residue. Rad6-Rad18-mediated PCNA monoubiquitination promotes...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Li F,Ball LG,Fan L,Hanna M,Xiao W

    更新日期:2018-04-01 00:00:00

  • Abortive activity of Topoisomerase I: a challenge for genome integrity?

    abstract::Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Jakobsen KP,Andersen AH,Bjergbæk L

    更新日期:2019-10-01 00:00:00

  • Genetics of carotene biosynthesis in Phycomyces.

    abstract::Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the differ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Roncero MI,Cerdá-Olmedo E

    更新日期:1982-05-01 00:00:00

  • Deletion of the Cochliobolus heterostrophus mating-type (MAT) locus promotes the function of MAT transgenes.

    abstract::Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Wirsel S,Turgeon BG,Yoder OC

    更新日期:1996-02-01 00:00:00

  • Characterization of the nuclear gene encoding chloroplast ribosomal protein S13 from Arabidopsis thaliana.

    abstract::We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal po...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kumar R,Drouaud J,Raynal M,Small I

    更新日期:1995-09-01 00:00:00

  • ALG/alg: a single gene controlling the utilization of lactate in the presence of antimycin in the yeast Saccharomyces cerevisiae.

    abstract::A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ferrero I,Rambaldelli R,Genga AM,Donnini C,Puglisi PP

    更新日期:1984-08-01 00:00:00

  • Fungal effectors, the double edge sword of phytopathogens.

    abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Pradhan A,Ghosh S,Sahoo D,Jha G

    更新日期:2020-11-04 00:00:00

  • Pentose-phosphate pathway in Saccharomyces cerevisiae: analysis of deletion mutants for transketolase, transaldolase, and glucose 6-phosphate dehydrogenase.

    abstract::Deletion mutants for the yeast transketolase gene TKL1 were constructed by gene replacement. Transketolase activity was below the level of detection in mutant crude extracts. Transketolase protein could be detected as a single protein band of the expected size by Western-blot analysis in wild-type strains but not in t...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Schaaff-Gerstenschläger I,Zimmermann FK

    更新日期:1993-11-01 00:00:00

  • Overexpression of Pa_1_10620 encoding a mitochondrial Podospora anserina protein with homology to superoxide dismutases and ribosomal proteins leads to lifespan extension.

    abstract::In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Grimm C,Böhl L,Osiewacz HD

    更新日期:2015-02-01 00:00:00

  • Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen, Ustilago maydis.

    abstract::A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transfo...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Keon JP,White GA,Hargreaves JA

    更新日期:1991-06-01 00:00:00

  • Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger.

    abstract::Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ngiam C,Jeenes DJ,Archer DB

    更新日期:1997-02-01 00:00:00

  • Genetic effects of photoactivated psoralens during meiosis in DNA repair mutant pso3-1 of Saccharomyces cerevisiae.

    abstract::The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Pothin HS,da Silva KV,Brendel M,Henriques JA

    更新日期:1994-01-01 00:00:00

  • Intramolecular recombination of a mitochondrial minicircular plasmid-like DNA of date-palm mediated by a set of short direct-repeat sequences.

    abstract::A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Benslimane AA,Hartmann C,Ouenzar B,Rode A

    更新日期:1996-05-01 00:00:00

  • Isolation and characterization of the EF-1 alpha gene of the filamentous fungus Puccinia graminis f. sp. tritici.

    abstract::A gene of Puccinia graminis f. sp. tritici, coding for the translation elongation factor 1 alpha (EF-1 alpha), was isolated from a P. graminis genomic library using the EF-1 alpha gene sequence of Absidia glauca. The coding region of 1389 nucleotides encodes a polypeptide of 463 amino acids and is interrupted by eight...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Schillberg S,Gross P,Tiburzy R

    更新日期:1995-03-01 00:00:00

  • Disruption of the cyclosporin synthetase gene of Tolypocladium niveum.

    abstract::Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Weber G,Leitner E

    更新日期:1994-11-01 00:00:00

  • Hyperresistance to DNA damaging agents in yeast.

    abstract::In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ruhland A,Brendel M,Haynes RH

    更新日期:1986-01-01 00:00:00

  • Mitochondrial genome of Saccharomyces douglasii: genes coding for components of the protein synthetic apparatus.

    abstract::Mitochondrial genes coding for some components of the protein synthetic apparatus in S. douglasii have been studies in detail. A region containing stretches of high homology to the S. cerevisiae tRNA synthesis locus (TSL) and the tRNA(fmet) gene has been identified and sequenced. The organization of this region was ve...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ragnini A,Grisanti P,Rinaldi T,Frontali L,Palleschi C

    更新日期:1991-03-01 00:00:00

  • Codon usage, genetic code and phylogeny of Dictyostelium discoideum mitochondrial DNA as deduced from a 7.3-kb region.

    abstract::We have sequenced a region (7,376-bp) of the mitochondrial (mt) DNA (54 kb) of the cellular slime mold, Dictyostelium discoideum. From the DNA and amino-acid sequence comparisons with known sequences, genes for ATPase subunit 9 (ATP9), cytochrome b (CYTB), NADH dehydrogenase subunits 1, 3 and 6 (ND1, ND3 and ND6), sma...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Angata K,Kuroe K,Yanagisawa K,Tanaka Y

    更新日期:1995-02-01 00:00:00

  • Starvation signals in yeast are integrated to coordinate metabolic reprogramming and stress response to ensure longevity.

    abstract::Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Zhang N,Cao L

    更新日期:2017-10-01 00:00:00

  • Conserved and non-conserved features among the yeast Ty elements.

    abstract::We have isolated and characterized a Ty element from a yeast cosmid library which exhibits several unusual features: it is flanked by non-homologous delta elements and directly associated with a singular delta element. A tRNA(Glu3) gene and tRNA(Cys) gene are found in conjunction with this element, located in opposite...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Stucka R,Hauber J,Feldmann H

    更新日期:1986-01-01 00:00:00

  • Amphimeric mitochondrial genomes of petite mutants of yeast. II. A model for the amplification of amphimeric mitochondrial petite DNA.

    abstract::A model for the recombination-directed replication and amplification of the mtDNA of amphimeric petite mutants of S. cerevisiae is proposed. Replication of an amphimeric master basic unit datA would be initiated in the inverted components a and A. The initiation of replication should be associated with the amphimeric ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Rayko E,Goursot R

    更新日期:1996-07-31 00:00:00

  • A downstream activator sequence regulates the expression of the yeast transposon.

    abstract::The regulation of transcription of the class II yeast transposon Ty 1-17 has been examined using a Ty::LEU2 fusion gene. Deletion analysis has defined a 251 bp region within the coding sequence of the TY A gene which is essential for Ty transcription and is also required for diploid control of Ty expression. This down...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Warmington JR,Oliver SG

    更新日期:1988-05-01 00:00:00

  • Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae.

    abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Haase E,Servos J,Brendel M

    更新日期:1992-04-01 00:00:00

  • Posttranscriptional regulation of Git1p, the glycerophosphoinositol/glycerophosphocholine transporter of Saccharomyces cerevisiae.

    abstract::Glycerophosphoinositol (GroPIns) and glycerophosphocholine (GroPCho) are the products of phospholipase-B mediated deacylation of phosphatidylinositol and phosphatidylcholine, respectively. GroPIns and GroPCho are transported across the Saccharomyces cerevisiae plasma membrane into the cell via the transporter encoded ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Almaguer C,Fisher E,Patton-Vogt J

    更新日期:2006-12-01 00:00:00

  • Characterisation of the LdsRNA encoded mRNA of yeast.

    abstract::Radioactive messenger RNA (mRNA) was isolated from a Saccharomyces cerevisiae strain containing the L double stranded RNA (dsRNA) species. This mRNA was hybridised to LdsRNA isolated from the same strain. Analysis of the hybrid formed shows it to be of a similar size to the LdsRNA. It is concluded from this result tha...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Haylock RW,Bevan EA

    更新日期:1981-12-01 00:00:00

  • Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria.

    abstract::A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Willmann A,Weiss M,Nehls U

    更新日期:2007-02-01 00:00:00

  • Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants.

    abstract::We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Martín H,Castellanos MC,Cenamor R,Sánchez M,Molina M,Nombela C

    更新日期:1996-05-01 00:00:00

  • Group I intron lateral transfer between red and brown algal ribosomal RNA.

    abstract::How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Bhattacarya D,Cannone JJ,Gutell RR

    更新日期:2001-08-01 00:00:00