Abstract:
:A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the core consensus is deleted. However, deletion mutations revealed that this near-match together with a short palindromic sequence, also situated in the B-flanking region, comprise a single element crucial for optimal ARS function. The palindrome has the potential of forming a stem-loop structure. Rather precise observations concerning the borders of the B-region were achieved. The four base pairs separating the near-match from the core consensus perform a spacing function where the identity of the bases are unimportant. However, this spacing is highly important since deletion of these four base pairs leads to an ARS- phenotype.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Kirpekar F,Gulløv Kdoi
10.1007/BF00351723subject
Has Abstractpub_date
1992-09-01 00:00:00pages
175-80issue
3eissn
0172-8083issn
1432-0983journal_volume
22pub_type
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