Molecular analysis of the transcripts encoding the light-harvesting chlorophyll a/b protein in Euglena gracilis: unusual size of the mRNA.


:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp long insert containing a poly A tail. On an amino-acid basis, the Euglena LHCP shows a 60% homology with the corresponding pea gene. Northern blot analysis revealed an unusual size of 7.5 kb. This extremely large mRNA is also recognized by a LHCP probe from Chlamydomonas. Hybrid-selected translation produces 2 proteins of 90-110 kd equivalent in size of the polypeptides immunoprecipitated from the translation products of polyadenylated RNAs.


Curr Genet


Current genetics


Houlne G,Schantz R




Has Abstract


1987-01-01 00:00:00












  • A downstream activator sequence regulates the expression of the yeast transposon.

    abstract::The regulation of transcription of the class II yeast transposon Ty 1-17 has been examined using a Ty::LEU2 fusion gene. Deletion analysis has defined a 251 bp region within the coding sequence of the TY A gene which is essential for Ty transcription and is also required for diploid control of Ty expression. This down...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Warmington JR,Oliver SG

    更新日期:1988-05-01 00:00:00

  • Structure of the rubisco operon from the multicellular red alga Antithamnion spec.

    abstract::In the multicellular red alga Antithamnion spec. both rubisco genes (rbcL and rbcS) are encoded on the plastid DNA (ptDNA). Both genes are separated by a short A/T-rich spacer of 100 bp and are cotranscribed into an mRNA of approximately 2.7 kb. These findings are in extensive agreement with those obtained from two un...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kostrzewa M,Valentin K,Maid U,Radetzky R,Zetsche K

    更新日期:1990-12-01 00:00:00

  • The isoprenoid pathway in the ectomycorrhizal fungus Tuber borchii Vittad.: cloning and characterisation of the tbhmgr, tbfpps and tbsqs genes.

    abstract::The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

    更新日期:2006-12-01 00:00:00

  • A conserved role of the RSC chromatin remodeler in the establishment of nucleosome-depleted regions.

    abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Yague-Sanz C,Vázquez E,Sánchez M,Antequera F,Hermand D

    更新日期:2017-05-01 00:00:00

  • Identification of an ADPG-dependent trehalose synthase in Saccharomyces.

    abstract::Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to differe...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Paschoalin VM,Silva JT,Panek AD

    更新日期:1989-08-01 00:00:00

  • Cloning and analysis of the nuclear gene MRP-S9 encoding mitochondrial ribosomal protein S9 of Saccharomyces cerevisiae.

    abstract::The Saccharomyces cerevisiae nuclear gene MRP-S9 was identified as part of the European effort in sequencing chromosome II. MRP-S9 encodes for a hydrophilic and basic protein of 278 amino acids with a molecular mass of 32 kDa. The C-terminal part (aa 153-278) of the MRP-S9 protein exhibits significant sequence similar...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kötter P,Entian KD

    更新日期:1995-06-01 00:00:00

  • Molecular karyotypes for Alternaria plant pathogens known to produce host-specific toxins.

    abstract::There are at least ten plant diseases caused by Alternaria species in which host-specific toxins (HSTs) are responsible for fungal pathogenicity. Of these HST-producers, seven are considered distinct pathotypes of the species Alternaria alternata, and the remaining three are among other species of pathogenic Alternari...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Akamatsu H,Taga M,Kodama M,Johnson R,Otani H,Kohmoto K

    更新日期:1999-07-01 00:00:00

  • A mobile group II intron of a naturally occurring rearranged mitochondrial genome in Kluyveromyces lactis.

    abstract::Mitochondrial intron content is variable in the yeast Kluyveromyces lactis. Strains can be divided into three classes depending on the structure of the cytochrome oxidase subunit 1 (COX1) gene: (1) those containing intron K1 cox1.1, (2) those containing K1 cox1.2, 3 and 4 and, (3) those that contain all four introns. ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Skelly PJ,Hardy CM,Clark-Walker GD

    更新日期:1991-07-01 00:00:00

  • L1-like non-LTR retrotransposons in the yeast Candida albicans.

    abstract::Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Goodwin TJ,Ormandy JE,Poulter RT

    更新日期:2001-04-01 00:00:00

  • The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : II. sup8 and sup10.

    abstract::Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Munz P,Dorsch-Häsler K,Leupold U

    更新日期:1983-04-01 00:00:00

  • Relationship of histidine sensitivity to DNA damage and stress induced responses in mutagen sensitive mutants of Neurospora crassa.

    abstract::Previous work in other laboratories has shown that several mutagen sensitive mutants of Neurospora crassa are extremely sensitive to low levels of histidine in the culture medium. We have shown that wild type Neurospora accumulates nicks or breaks in the DNA in the presence of histidine. The number of nicks accumulati...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Howard CA,Baker TI

    更新日期:1988-05-01 00:00:00

  • The yeast ORF YDL202w codes for the mitochondrial ribosomal protein YmL11.

    abstract::The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Bui DM,Jarosch E,Schweyen RJ

    更新日期:1997-05-01 00:00:00

  • Abortive activity of Topoisomerase I: a challenge for genome integrity?

    abstract::Single-strand breaks (SSB) are discontinuities in one strand of the DNA double helix and are the most common type of damages that arise in cells. SSBs arise mainly from direct attack by intracellular metabolites, however, also essential nuclear processes generate SSBs as intermediates. During the catalytic cycle of DN...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Jakobsen KP,Andersen AH,Bjergbæk L

    更新日期:2019-10-01 00:00:00

  • Prokaryotic promoters in the chloroplast DNA replication origin of Chlamydomonas reinhardtii.

    abstract::In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Wu M,Kong XF,Kung SD

    更新日期:1986-01-01 00:00:00

  • Mutations at three different nuclear loci of Chlamydomonas suppress a defect in chloroplast psbD mRNA accumulation.

    abstract::In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Nickelsen J

    更新日期:2000-02-01 00:00:00

  • Regulation of bacterial gene expression by ribosome stalling and rescuing.

    abstract::Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Jin Y,Jin S,Wu W

    更新日期:2016-05-01 00:00:00

  • Identification and characterization of CEN12 in the budding yeast Saccharomyces cerevisiae.

    abstract::In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Gammie AE,Rose MD

    更新日期:1995-11-01 00:00:00

  • Genetics of carotene biosynthesis in Phycomyces.

    abstract::Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the differ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Roncero MI,Cerdá-Olmedo E

    更新日期:1982-05-01 00:00:00

  • Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p.

    abstract::Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kerkmann K,Lehming N

    更新日期:2001-07-01 00:00:00

  • Isolation and genetical and biochemical characterization of mutants resistant to the alkaloid lycorine.

    abstract::Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is responsible for the re...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Del Giudice L,Massardo DR,Manna F,Wolf K

    更新日期:1986-01-01 00:00:00

  • Identification of extragenic suppressors of the cif1 mutation in Saccharomyces cerevisiae.

    abstract::The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Blázquez MA,Gancedo C

    更新日期:1994-02-01 00:00:00

  • Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein.

    abstract::Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Régnacq M,Boucherie H

    更新日期:1993-05-01 00:00:00

  • The repeatability of genome-wide mutation rate and spectrum estimates.

    abstract::Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Behringer MG,Hall DW

    更新日期:2016-08-01 00:00:00

  • Cloning of the mating type locus from Ascochyta lentis (teleomorph: Didymella lentis) and development of a multiplex PCR mating assay for Ascochyta species.

    abstract::The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Chérif M,Chilvers MI,Akamatsu H,Peever TL,Kaiser WJ

    更新日期:2006-09-01 00:00:00

  • Amphimeric mitochondrial genomes of petite mutants of yeast. II. A model for the amplification of amphimeric mitochondrial petite DNA.

    abstract::A model for the recombination-directed replication and amplification of the mtDNA of amphimeric petite mutants of S. cerevisiae is proposed. Replication of an amphimeric master basic unit datA would be initiated in the inverted components a and A. The initiation of replication should be associated with the amphimeric ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Rayko E,Goursot R

    更新日期:1996-07-31 00:00:00

  • Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger.

    abstract::Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ngiam C,Jeenes DJ,Archer DB

    更新日期:1997-02-01 00:00:00

  • ALG/alg: a single gene controlling the utilization of lactate in the presence of antimycin in the yeast Saccharomyces cerevisiae.

    abstract::A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ferrero I,Rambaldelli R,Genga AM,Donnini C,Puglisi PP

    更新日期:1984-08-01 00:00:00

  • Cloning and characterization of the nuclear gene encoding plastid glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa.

    abstract::The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 b...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Zhou YH,Ragan MA

    更新日期:1994-07-01 00:00:00

  • Unexpectedly long 14S ribosomal RNA gene in Tetrahymena mitochondria.

    abstract::Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by alig...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Labriola J,Weiss I,Zapatero J,Suyama Y

    更新日期:1987-01-01 00:00:00

  • Characterization of mating type genes supports the hypothesis that Stagonosporopsis chrysanthemi is homothallic and provides evidence that Stagonosporopsis tanaceti is heterothallic.

    abstract::To understand the organization of the mating type locus of Stagonosporopsis tanaceti and Stagonosporopsis chrysanthemi, and its potential role in the epidemiology of ray blight of pyrethrum and chrysanthemum, respectively, the mating type (MAT) locus of these species was cloned and characterized using PCR-based techni...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Chilvers MI,Jones S,Meleca J,Peever TL,Pethybridge SJ,Hay FS

    更新日期:2014-11-01 00:00:00