Abstract：
:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp long insert containing a poly A tail. On an amino-acid basis, the Euglena LHCP shows a 60% homology with the corresponding pea gene. Northern blot analysis revealed an unusual size of 7.5 kb. This extremely large mRNA is also recognized by a LHCP probe from Chlamydomonas. Hybrid-selected translation produces 2 proteins of 90-110 kd equivalent in size of the polypeptides immunoprecipitated from the translation products of polyadenylated RNAs.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Houlne G,Schantz Rdoi
10.1007/BF00368064subject
Has Abstractpub_date
1987-01-01 00:00:00pages
611-6issue
8eissn
0172-8083issn
1432-0983journal_volume
12pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract：:URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in an Escherichia coli-Schizosaccharomyces pombe (E. coli-S. pombe) replicative plasmid to a 1.76 kb HindIII fragment. This plasmid is maintained at a high co...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00419562
更新日期：1987-01-01 00:00:00
abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050371
更新日期：1998-08-01 00:00:00
abstract：:Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00418409
更新日期：1986-01-01 00:00:00
abstract：:A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transfo...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312739
更新日期：1991-06-01 00:00:00
abstract：:Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00317923
更新日期：1992-10-01 00:00:00
abstract：:Like other microorganisms, free-living Candida albicans is mainly present in a three-dimensional multicellular structure, which is called a biofilm, rather than in a planktonic form. Candida albicans biofilms can be isolated from both abiotic and biotic surfaces at various locations within the host. As the number of a...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-013-0400-3
更新日期：2013-11-01 00:00:00
abstract：:The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940000130
更新日期：2000-07-01 00:00:00
abstract：:A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acid...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050323
更新日期：1998-03-01 00:00:00
abstract：:1710 asci exhibiting mendelian or aberrant segregation at the b8 spore colour locus in Ascobolus were analysed from crosses between b8 mutants and the wild type in the presence of linked flanking markers. The frequencies of associated crossing-over were found to vary with the various classes of aberrant segregation an...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00435213
更新日期：1982-11-01 00:00:00
abstract：:Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-002-0316-9
更新日期：2002-08-01 00:00:00
abstract：:The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cel...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF02221519
更新日期：1996-04-01 00:00:00
abstract：:For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-006-0073-2
更新日期：2006-07-01 00:00:00
abstract：:Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-015-0545-3
更新日期：2016-05-01 00:00:00
abstract：:A double-stranded (ds)RNA, designated as M2, is associated with hypovirulence, conversion of the quinic acid pathway from inducible to constitutive and downregulation of the shikimic acid pathway in the Rhizoctonia solani culture Rhs 1A1. In this study, we report that in the virulent, M2-lacking isolate Rhs 1AP, which...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-003-0375-6
更新日期：2003-05-01 00:00:00
abstract：:Phosphatidylcholines (PCs) are a class of major cell membrane phospholipids that participate in many physiological processes. Three genes, choA, choB and choC, have been proposed to function in the endogenous biosynthesis of PC in Aspergillus nidulans. In this study, we characterize the choC gene encoding a putative h...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-010-0300-8
更新日期：2010-06-01 00:00:00
abstract：:Virus-like particles (VLPs) of 40 nm diameter were isolated from the yeast-like fungus Dipodascus magnusii. These VLPs copurify with several linear double-stranded RNA molecules of different size. We have found some polymorphism in both the length and the number of these dsRNAs among six D. magnusii strains. Analysis ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351499
更新日期：1993-03-01 00:00:00
abstract：:RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351783
更新日期：1994-05-01 00:00:00
abstract：:La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312776
更新日期：1991-07-01 00:00:00
abstract：:Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-007-0158-6
更新日期：2007-11-01 00:00:00
abstract：:Most eukaryotes require molecular oxygen for growth. In general, oxygen is the terminal electron acceptor of the respiratory chain and represents an important substrate for the biosynthesis of cellular compounds. However, in their natural environment, such as soil, and also during the infection, filamentous fungi are ...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-015-0487-9
更新日期：2015-08-01 00:00:00
abstract：:The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, th...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-009-0274-6
更新日期：2009-12-01 00:00:00
abstract：:The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-005-0010-9
更新日期：2005-09-01 00:00:00
abstract：:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336744
更新日期：1993-01-01 00:00:00
abstract：:We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-011-0363-1
更新日期：2012-02-01 00:00:00
abstract：:The gene for subunit II of cytochrome oxidase in the yeast Hansenula saturnus was previously shown to be located on a 1.7 kb HindIII-BamHI fragment of mitochondrial DNA (Lawson and Deters, accompanying paper). In this paper, we report the nucleotide sequence of a large part of this fragment, covering the coding region...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00421605
更新日期：1985-01-01 00:00:00
abstract：:In Saccharomyces cerevisiae, the HGS2-1 allele confers sensitivities to inorganis mercury (Ono and Sakamoto 1985) and to excess fermentable sugars such as glucose (Sakamoto et al. 1985); exogenous tyrosine antagonizes both inorganic mercury and excess glucose. In this study, the inorganic mercury sensitive strain has ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00378183
更新日期：1987-01-01 00:00:00
abstract：:The wheat mitochondrial (mt) cox3 has been localized and sequenced. The gene exists as a single copy in the wheat mt master chromosome and is transcribed into a single 1.2 kb RNA, whose extremities have been mapped. Comparison of the wheat and Oenothera cox3 sequences gives ambiguous indications concerning the amino a...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00313247
更新日期：1990-01-01 00:00:00
abstract：:The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a pote...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940000160
更新日期：2000-12-01 00:00:00
abstract：:Spontaneous and double-strand break (DSB)-induced gene conversion in Saccharomyces cerevisiae was assayed using non-tandem chromosomal direct repeat crosses and plasmid x chromosome crosses. Each cross involved identical ura3 alleles marked with phenotypically silent restriction fragment length polymorphic (RFLP) muta...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050396
更新日期：1998-10-01 00:00:00
abstract：:In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (f...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00365705
更新日期：1982-07-01 00:00:00
