Abstract：
:The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integration and repair by an homologous cross-over with plasmid containing a double-strand break or gap in the PYR3 gene was markedly reduced in the absence of the REC1 gene product. Homologous replacement of the chromosomal pyr3-1 allele by a single copy of wild-type sequences from plasmid cut outside PYR3 was not found in the absence of the REC1 product. Instead, novel transformants generated in its absence suggests that ligation plays a role in their generation.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Banks GR,Kanuga N,Ealing J,Spanos A,Holden DWdoi
10.1007/BF00326414subject
Has Abstractpub_date
1992-12-01 00:00:00pages
483-9issue
6eissn
0172-8083issn
1432-0983journal_volume
22pub_type
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