Suppression of a yeast mitochondrial RNA processing defect by nuclear mutations.

abstract：:The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

journal_title：Current genetics

authors： Blázquez MA,Gancedo C

更新日期：1994-02-01 00:00:00

abstract：:Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

journal_title：Current genetics

authors： D'Souza AD,Sultana S,Maheshwari R

更新日期：2005-03-01 00:00:00

abstract：:An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...

journal_title：Current genetics

authors： Quesada H,Skibinski DA,Skibinski DO

更新日期：1996-04-01 00:00:00

abstract：:In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standa...

journal_title：Current genetics

authors： Zeppa S,Guidi C,Zambonelli A,Potenza L,Vallorani L,Pierleoni R,Sacconi C,Stocchi V

更新日期：2002-12-01 00:00:00

abstract：:Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...

journal_title：Current genetics

authors： Ngiam C,Jeenes DJ,Archer DB

更新日期：1997-02-01 00:00:00

abstract：:The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...

journal_title：Current genetics

authors： Chérif M,Chilvers MI,Akamatsu H,Peever TL,Kaiser WJ

更新日期：2006-09-01 00:00:00

abstract：:In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate for numerous regulatory post-translational modifications. However, the biological...

journal_title：Current genetics

authors： Ichikawa Y,Kaufman PD

更新日期：2019-04-01 00:00:00

abstract：:A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

journal_title：Current genetics

authors： Willmann A,Weiss M,Nehls U

更新日期：2007-02-01 00:00:00

abstract：:The cloning and characterization of the Thcut1 gene, which encodes a cutinase protein of the biocontrol fungus Trichoderma harzianum T34, is reported. Cutinases, which are secreted enzymes that hydrolyse cutin, belong to a class of serine esterases able to hydrolyze fatty acid esters and emulsified triglycerides. The ...

journal_title：Current genetics

authors： Rubio MB,Cardoza RE,Hermosa R,Gutiérrez S,Monte E

更新日期：2008-12-01 00:00:00

abstract：:O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...

journal_title：Current genetics

authors： Zakrzewska A,Migdalski A,Saloheimo M,Penttila ME,Palamarczyk G,Kruszewska JS

更新日期：2003-04-01 00:00:00

abstract：:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...

journal_title：Current genetics

authors： Ruhland A,Brendel M,Haynes RH

更新日期：1986-01-01 00:00:00

abstract：:Chloroplast DNAs from six different laboratory collections of Euglena gracilis "strain Z" and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosom...

journal_title：Current genetics

authors： Wurtz EA,Buetow DE

更新日期：1981-07-01 00:00:00

abstract：:Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

journal_title：Current genetics

authors： Hendrickson PG,Silliker ME

更新日期：2010-06-01 00:00:00

abstract：:We have sequenced a region (7,376-bp) of the mitochondrial (mt) DNA (54 kb) of the cellular slime mold, Dictyostelium discoideum. From the DNA and amino-acid sequence comparisons with known sequences, genes for ATPase subunit 9 (ATP9), cytochrome b (CYTB), NADH dehydrogenase subunits 1, 3 and 6 (ND1, ND3 and ND6), sma...

journal_title：Current genetics

authors： Angata K,Kuroe K,Yanagisawa K,Tanaka Y

更新日期：1995-02-01 00:00:00

abstract：:Although the mitochondrial genomes of the Chinese Spring and Aquila varieties of wheat are normally similar in organization, this is not so in tissue cultures initiated from their immature embryos where the mitochondrial genomes of both are rearranged and in different, characteristic, ways. However, the mitochondrial ...

journal_title：Current genetics

authors： Hartmann C,De Buyser J,Henry Y,Morère-Le Paven MC,Dyer TA,Rode A

更新日期：1992-05-01 00:00:00

abstract：:In eukaryotes, the ubiquitin-like (UBL) protein-activating enzymes play a crucial role in autophagy process, however, it is poorly characterized in filamentous fungi. Here, we investigated the functions of two UBL activating enzymes, BcAtg3 (E2) and BcAtg7 (E1) in the plant pathogenic fungus Botrytis cinerea. The phys...

journal_title：Current genetics

authors： Ren W,Sang C,Shi D,Song X,Zhou M,Chen C

更新日期：2018-08-01 00:00:00

abstract：:Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as bls1 and bls2, respectively. A gene bls1 was linked to an ilv3 gene located on the right a...

journal_title：Current genetics

authors： Ishiguro J,Miyazaki M

更新日期：1985-01-01 00:00:00

abstract：:The complete nucleotide sequence of the Morus mongolica chloroplast (cp) genome was reported and characterized in this study. The cp genome is a circular molecule of 158,459 bp containing a pair of 25,678 bp IR regions, separated by small and large single-copy regions of 19,736 and 87,363 bp, respectively. The number ...

journal_title：Current genetics

authors： Kong W,Yang J

更新日期：2016-02-01 00:00:00

abstract：:We have characterised a cDNA clone and a nuclear gene encoding the chloroplast 30 s ribosomal protein S13 from Arabidopsis thaliana. The identification is based on the high similarity of the predicted amino-acid sequence with eubacterial S13 protein sequences, and immunodetection of a 14.5-kDa chloroplast ribosomal po...

journal_title：Current genetics

authors： Kumar R,Drouaud J,Raynal M,Small I

更新日期：1995-09-01 00:00:00

abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...

journal_title：Current genetics

authors： Crowley JH,Tove S,Parks LW

更新日期：1998-08-01 00:00:00

abstract：:Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...

journal_title：Current genetics

authors： Yushenova IA,Arkhipova IR

更新日期：2018-12-01 00:00:00

abstract：:In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...

journal_title：Current genetics

authors： Grimm C,Böhl L,Osiewacz HD

更新日期：2015-02-01 00:00:00

abstract：:The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...

journal_title：Current genetics

authors： Yague-Sanz C,Vázquez E,Sánchez M,Antequera F,Hermand D

更新日期：2017-05-01 00:00:00

abstract：:Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...

journal_title：Current genetics

authors： Akashi K,Sakurai K,Hirayama J,Fukuzawa H,Ohyama K

更新日期：1996-07-31 00:00:00

abstract：:The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

journal_title：Current genetics

authors： Howe CJ

更新日期：1985-01-01 00:00:00

abstract：:Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

journal_title：Current genetics

authors： Zhang N,Cao L

更新日期：2017-10-01 00:00:00

abstract：:The atpB and atpE genes encode beta and epsilon subunits, respectively, of chloroplast ATP synthase and are co-transcribed in the plant species so far studied. In tobacco, an atpB gene-specific probe hybridizes to 2.7- and 2.3-kb transcripts. In addition to these, a probe from the atpE coding region hybridizes also to...

journal_title：Current genetics

authors： Kapoor S,Wakasugi T,Deno H,Sugiura M

更新日期：1994-09-01 00:00:00

abstract：:There are at least ten plant diseases caused by Alternaria species in which host-specific toxins (HSTs) are responsible for fungal pathogenicity. Of these HST-producers, seven are considered distinct pathotypes of the species Alternaria alternata, and the remaining three are among other species of pathogenic Alternari...

journal_title：Current genetics

authors： Akamatsu H,Taga M,Kodama M,Johnson R,Otani H,Kohmoto K

更新日期：1999-07-01 00:00:00

abstract：:Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...

journal_title：Current genetics

authors： Lee S,Son H,Lee J,Lee YR,Lee YW

更新日期：2011-10-01 00:00:00

abstract：:The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...

journal_title：Current genetics

authors： Hawkins AR

更新日期：1987-01-01 00:00:00