Abstract：
:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recombinant plasmids were rescued into Escherichia coli and subjected to restriction mapping and sequence analysis. It was shown that circular DNA molecules recombined predominantly within homologous fragments. Linear DNA fragments integrated into circular plasmids by invasion of their ends into random non-homologous sites, but exhibited some bias in choice of a target sequence. Cointegrates of multiple plasmid copies were often observed. In some of the plasmids analysed, short duplications of the target sequence flanking an inserted linear DNA fragment have been revealed.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Aleksenko AYdoi
10.1007/BF00310500subject
Has Abstractpub_date
1994-10-01 00:00:00pages
352-8issue
4eissn
0172-8083issn
1432-0983journal_volume
26pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract：:A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00324663
更新日期：1993-07-01 00:00:00
abstract：:The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336850
更新日期：1992-03-01 00:00:00
abstract：:In the wood destroying fungus Schizophyllum commune, a well known subject for genetic studies, fruit bodies are produced not only in the course of the sexual cycle but also asexually. Sexual fruiting requires the establishment of a dikaryon which is under the control of the incompatibility factors A and B. Asexual fru...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00413309
更新日期：1979-12-01 00:00:00
abstract：:Cochliobolus heterostrophus has alternate genes (MAT-1 and MAT-2) at its mating-type locus. Transformants of a MAT-1 or a MAT-2 strain carrying a transgene of opposite mating type can self and are dual maters; the transgene, however, promotes development of pseudothecia only, not ascospores. To determine if the reside...
journal_title：Current genetics
pub_type： 杂志文章
doi：
更新日期：1996-02-01 00:00:00
abstract：:Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-015-0511-0
更新日期：2016-02-01 00:00:00
abstract：:La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312776
更新日期：1991-07-01 00:00:00
abstract：:Cell aging is the result of deteriorating competence in maintaining cellular homeostasis and quality control. Certain cell types are able to rejuvenate through asymmetric cell division by excluding aging factors, including damaged cellular compartments and extrachromosomal rDNA circles, from entering the daughter cell...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-020-01133-4
更新日期：2020-11-27 00:00:00
abstract：:Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-020-01053-3
更新日期：2020-08-01 00:00:00
abstract：:Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-002-0316-9
更新日期：2002-08-01 00:00:00
abstract：:In fungi, sexual reproduction primarily depends on the interaction between peptide pheromones and their receptors. Most ascomycete fungi produce two classes of peptide mating pheromones, a simple peptide and a modified peptide. These peptides are recognized by their corresponding receptors on the surface of cells of t...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-019-00968-w
更新日期：2019-10-01 00:00:00
abstract：:The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050221
更新日期：1997-05-01 00:00:00
abstract：:Most organisms prefer to utilize glucose as a carbon source. Accordingly, the expression of genes involved in the catabolism of other carbon sources is repressed by the presence of glucose in a process known as (carbon) catabolite repression. However, much less is known about the relationships between "poor" carbon so...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-019-00996-6
更新日期：2019-12-01 00:00:00
abstract：:The directed and controlled switching of mating type which occurs in homothallic forms of the yeast Saccharomyces cerevisiaee is dependent upon the presence of the wild type RAD52 gene. The RAD52 gene is also required for spontaneous mitotic and meiotic recombination. It has been observed that the two haploid mating t...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00377609
更新日期：1983-11-01 00:00:00
abstract：:Arenicin-1, a 21-mer antimicrobial peptide exerts significant broad-spectrum antimicrobial activity with membrane-active mechanisms. However, owing to multiple mechanisms of cell death, the antibacterial mechanism of arenicin-1 requires detailed analysis. In the present study, arenicin-1-treated bacteria underwent an ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-018-0855-3
更新日期：2019-02-01 00:00:00
abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00309601
更新日期：1991-05-01 00:00:00
abstract：:In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050511
更新日期：1999-12-01 00:00:00
abstract：:The nucleotide sequences of regions containing the mating-type locus of the plant-pathogenic ascomycete Leptosphaeria maculans are described. The MAT1-1 gene is 1,368 bp, encoding a predicted protein of 441 amino acids, with a 45-bp intron. The MAT1-2 gene is 1,246 bp, encoding a predicted protein of 397 amino acids, ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-003-0391-6
更新日期：2003-08-01 00:00:00
abstract：:Unlike most polymorphic markers in the Chlamydomonas eugametos and Chlamydomonas moewusii chloroplast DNAs (cpDNAs), the C. moewusii 6- and 21-kb extra sequences and the C. eugametos-specific CeLSU small middle dot 5 intron are transmitted to all of the few viable progeny in reciprocal crosses between the two green al...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050144
更新日期：1996-09-01 00:00:00
abstract：:In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00518162
更新日期：1995-11-01 00:00:00
abstract：:Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the differ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00445733
更新日期：1982-05-01 00:00:00
abstract：:An electrophoretic karyotype of the filamentous fungus Podospora anserina has been obtained using contour-clamped homogeneous electric field gel electrophoresis. Six chromosomal bands were separated with one migrating as a doublet. The size of the chromosomes was estimated to be between 3.8 and 6.0 megabase pairs (mb)...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351795
更新日期：1993-09-01 00:00:00
abstract：:The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351744
更新日期：1992-07-01 00:00:00
abstract：:The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00384611
更新日期：1987-01-01 00:00:00
abstract：:In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-005-0048-8
更新日期：2006-04-01 00:00:00
abstract：:A gene encoding mitochondrial S13 is generally present in the mitochondrial genome of higher plants, but is lacking from the Arabidopsis thaliana mitochondrial genome. Previous research has failed to identify a nuclear gene capable of encoding a mitochondrial S13 protein or the protein itself. Doubts have even been ra...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-002-0271-5
更新日期：2002-03-01 00:00:00
abstract：:Botryotinia fuckeliana, the causal agent of grey mould, was biolistically transformed to hygromycin B resistance using a plasmid (pOHT) containing a bacterial hygromycin phosphotransferase gene fused to regulatory sequences from Aspergillus nidulans. Multiple copies of the plasmid, precipitated onto tungsten particles...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00309537
更新日期：1994-02-01 00:00:00
abstract：:Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referre...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00376740
更新日期：1988-09-01 00:00:00
abstract：:The microtubule-organizing center (MTOC) is the organelle that manages the distribution of microtubule filaments in the cell. It includes such centers of microtubule organization as basal bodies, nucleus-associated bodies, spindle pole bodies and centrosomes. The centrosome is the MTOC in most animal cells. Despite th...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-001-0263-x
更新日期：2002-02-01 00:00:00
abstract：:Saccharomyces cerevisiae adapts to oxidative, osmotic stress and nutrient deprivation through transcriptional changes, decreased proliferation, and entry into other developmental pathways such as pseudohyphal formation and sporulation. Inositol pyrophosphates are necessary for these cellular responses. Inositol pyroph...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-020-01078-8
更新日期：2020-10-01 00:00:00
abstract：:We find three interesting characteristics of the genes encoding the ribosomal RNA (rRNA) in the basidiomycete Coprinus cinereus. First, there are only 60 to 90 copies of the genes, fewer than in other fungi. Second, the genes are organized in an unusual arrangement. The 5S rRNA genes are located in the repeat unit whi...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00395706
更新日期：1984-10-01 00:00:00
