Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.


:Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recombinant plasmids were rescued into Escherichia coli and subjected to restriction mapping and sequence analysis. It was shown that circular DNA molecules recombined predominantly within homologous fragments. Linear DNA fragments integrated into circular plasmids by invasion of their ends into random non-homologous sites, but exhibited some bias in choice of a target sequence. Cointegrates of multiple plasmid copies were often observed. In some of the plasmids analysed, short duplications of the target sequence flanking an inserted linear DNA fragment have been revealed.


Curr Genet


Current genetics


Aleksenko AY




Has Abstract


1994-10-01 00:00:00












  • An adaptive response to alkylating agents in Aspergillus nidulans.

    abstract::A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Hooley P,Shawcross SG,Strike P

    更新日期:1988-11-01 00:00:00

  • Mitochondrial genome of Saccharomyces douglasii: genes coding for components of the protein synthetic apparatus.

    abstract::Mitochondrial genes coding for some components of the protein synthetic apparatus in S. douglasii have been studies in detail. A region containing stretches of high homology to the S. cerevisiae tRNA synthesis locus (TSL) and the tRNA(fmet) gene has been identified and sequenced. The organization of this region was ve...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ragnini A,Grisanti P,Rinaldi T,Frontali L,Palleschi C

    更新日期:1991-03-01 00:00:00

  • Cytochrome c 1-deficient mutants in Saccharomyces cerevisiae.

    abstract::1. Nuclear mutants lacking cytochrome c l spectrum have been isolated. 2. The electron flow through the respiratory chain of the mutants is defect in the cytochrome bc 1-segment. 3. Complementation analysis has yielded two major complementation groups. 4. Tetrade analysis has established the two complementation groups...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Lang BF,Kaudewitz F

    更新日期:1982-12-01 00:00:00

  • A small gene family in barley encodes ribosomal proteins homologous to yeast YL17 and L22 from archaebacteria, eubacteria, and chloroplasts.

    abstract::The amino acid sequences of two barley ribosomal proteins, termed HvL17-1 and HvL17-2, were decoded from green leaf cDNA clones. The N-terminal sequences of the derived barley proteins are 48% identical to the N-terminal amino acid sequence of protein YL17 from the large subunit of yeast cytoplasmic ribosomes. Via arc...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Madsen LH,Kreiberg JD,Gausing K

    更新日期:1991-05-01 00:00:00

  • Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level.

    abstract::DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Raeder U,Broda P

    更新日期:1984-09-01 00:00:00

  • Molecular analysis of the transcripts encoding the light-harvesting chlorophyll a/b protein in Euglena gracilis: unusual size of the mRNA.

    abstract::A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Houlne G,Schantz R

    更新日期:1987-01-01 00:00:00

  • Regulation of mitochondrial transcription during the stringent response in yeast.

    abstract::In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Cantwell R,McEntee CM,Hudson AP

    更新日期:1992-03-01 00:00:00

  • Suppression of a yeast mitochondrial RNA processing defect by nuclear mutations.

    abstract::The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Smooker PM,Macreadie IJ,Wright JL,Lukins HB

    更新日期:1994-03-01 00:00:00

  • Fission yeast Cdc23 interactions with DNA replication initiation proteins.

    abstract::Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Hart EA,Bryant JA,Moore K,Aves SJ

    更新日期:2002-08-01 00:00:00

  • Natural translocation of a large segment of chromosome III to chromosome I in a laboratory strain of Saccharomyces cerevisiae.

    abstract::We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Camasses A

    更新日期:1996-08-01 00:00:00

  • Unexpectedly long 14S ribosomal RNA gene in Tetrahymena mitochondria.

    abstract::Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by alig...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Labriola J,Weiss I,Zapatero J,Suyama Y

    更新日期:1987-01-01 00:00:00

  • A gene transfer system based on the homologous pyrG gene and efficient expression of bacterial genes in Aspergillus oryzae.

    abstract::A homologous transformation system for Aspergillus oryzae is described. The system is based on an A. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrG) and the vector pAO4-2, which contains a functional A. oryzae pyrG gene as selection marker. Transformation of the A. oryzae pyrG mutant with circul...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: de Ruiter-Jacobs YM,Broekhuijsen M,Unkles SE,Campbell EI,Kinghorn JR,Contreras R,Pouwels PH,van den Hondel CA

    更新日期:1989-09-01 00:00:00

  • Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219.

    abstract::Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Dobson MJ,Futcher AB,Cox BS

    更新日期:1980-12-01 00:00:00

  • Transcriptome analysis of Aspergillus fumigatus exposed to voriconazole.

    abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: da Silva Ferreira ME,Malavazi I,Savoldi M,Brakhage AA,Goldman MH,Kim HS,Nierman WC,Goldman GH

    更新日期:2006-07-01 00:00:00

  • Screening for amyloid proteins in the yeast proteome.

    abstract::The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ryzhova TA,Sopova JV,Zadorsky SP,Siniukova VA,Sergeeva AV,Galkina SA,Nizhnikov AA,Shenfeld AA,Volkov KV,Galkin AP

    更新日期:2018-04-01 00:00:00

  • A DNA polymerase activity with characteristics of a reverse transcriptase in Podospora anserina.

    abstract::In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Steinhilber W,Cummings DJ

    更新日期:1986-01-01 00:00:00

  • Structure of the rubisco operon from the multicellular red alga Antithamnion spec.

    abstract::In the multicellular red alga Antithamnion spec. both rubisco genes (rbcL and rbcS) are encoded on the plastid DNA (ptDNA). Both genes are separated by a short A/T-rich spacer of 100 bp and are cotranscribed into an mRNA of approximately 2.7 kb. These findings are in extensive agreement with those obtained from two un...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kostrzewa M,Valentin K,Maid U,Radetzky R,Zetsche K

    更新日期:1990-12-01 00:00:00

  • Intron-encoded open reading frame of the GIY-YIG subclass in a plastid gene.

    abstract::Group-I introns, containing open reading frames (ORFs) that code for homing endonucleases, are widely distributed amongst eukaryotic organellar genomes. However, endonucleases of the GIY-YIG subclass have a restricted distribution in mitochondria and bacteriophages, and have never been observed in plastids. We have fo...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Paquin B,O'Kelly CJ,Lang BF

    更新日期:1995-06-01 00:00:00

  • Isolation and characterization of yeast mutants with thermoconditional sensitivity to the bifunctional alkylating agent nitrogen mustard.

    abstract::Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Siede W,Brendel M

    更新日期:1981-11-01 00:00:00

  • The influence of the Ustilago maydis REC1 gene on plasmid-chromosome recombination.

    abstract::The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Banks GR,Kanuga N,Ealing J,Spanos A,Holden DW

    更新日期:1992-12-01 00:00:00

  • A direct study of the relative synthesis of petite and grande mitochondrial DNA in zygotes from crosses involving suppressive petite mutants of Saccharomyces cerevisiae.

    abstract::Work in recent years has produced indirect evidence to support the view that the phenomenon of suppressiveness in yeast is the result of the ability of the petite mtDNA to out-replicate the wild-type genome. We have developed a method, based on fluorography of gels containing restriction fragments of radioactively lab...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Chambers P,Gingold E

    更新日期:1986-01-01 00:00:00

  • Identification of a chloroplast-encoded secA gene homologue in a chromophytic alga: possible role in chloroplast protein translocation.

    abstract::SecA is one of seven Sec proteins that comprise the prokaryotic protein translocation apparatus. A chloroplast-encoded secA gene has been identified from the unicellular chromophytic alga Pavlova lutherii. The gene predicts a protein that is related to the SecA proteins of Escherichia coli and Bacillus subtilis. The p...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Scaramuzzi CD,Hiller RG,Stokes HW

    更新日期:1992-11-01 00:00:00

  • A new gene encoding tRNA(Pro) (GGG) is present in the chloroplast genome of black pine: a compilation of 32 tRNA genes from black pine chloroplasts.

    abstract::The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Tsudzuki J,Ito S,Tsudzuki T,Wakasugi T,Sugiura M

    更新日期:1994-08-01 00:00:00

  • Tuning the sequence specificity of a transcription terminator.

    abstract::The bacterial hexameric helicase known as Rho is an archetypal sequence-specific transcription terminator that typically halts the synthesis of a defined set of transcripts, particularly those bearing cytosine-rich 3'-untranslated regions. However, under conditions of translational stress, Rho can also terminate trans...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Lawson MR,Berger JM

    更新日期:2019-06-01 00:00:00

  • Deletion of RNQ1 gene reveals novel functional relationship between divergently transcribed Bik1p/CLIP-170 and Sfi1p in spindle pole body separation.

    abstract::Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Strawn LA,True HL

    更新日期:2006-12-01 00:00:00

  • Transformations of Penicillium islandicum and Penicillium frequentans that produce anthraquinone-related compounds.

    abstract::Wild-type strains of Penicillium islandicum and Penicillium frequentans, which produce anthraquinone and related compounds, were transformed to benomyl and hygromycin B resistance. Plasmids pSV50 and pBT6, with benomyl-resistant beta-tublin genes, and plasmids pAN7-1 and pDH25, with a bacterial hygromycin phosphotrans...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Huang KX,Iwakami N,Fujii I,Ebizuka Y,Sankawa U

    更新日期:1995-11-01 00:00:00

  • A 7.5-kbp region of the maize (T cytoplasm) mitochondrial genome contains a chloroplast-like trnI (CAT) pseudo gene and many short segments homologous to chloroplast and other known genes.

    abstract::The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Zheng D,Nielsen BL,Daniell H

    更新日期:1997-08-01 00:00:00

  • L1-like non-LTR retrotransposons in the yeast Candida albicans.

    abstract::Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Goodwin TJ,Ormandy JE,Poulter RT

    更新日期:2001-04-01 00:00:00

  • Cloning and expression of Hormoconis resinae glucoamylase P cDNA in Saccharomyces cerevisiae.

    abstract::A cDNA coding for glucoamylase P of Hormoconis resinae was cloned using a synthetic oligonucleotide probe coding for a peptide fragment of the purified enzyme and polyclonal anti-glucoamylase antibodies. Nucleotide-sequence analysis revealed an open reading frame of 1848 base pairs coding for a protein of 616 amino-ac...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Vainio AE,Torkkeli HT,Tuusa T,Aho SA,Fagerström BR,Korhola MP

    更新日期:1993-07-01 00:00:00

  • Genetic transformation of Ascochyta rabiei using Agrobacterium-mediated transformation.

    abstract::In order to study pathogenic mechanisms of the plant pathogen Ascochyta rabiei, conditions for efficient transformation using Agrobacterium-mediated transformation were investigated. Hygromycin B resistance (hph) was superior to geneticin resistance (nptII) for selecting transformants, and the hph gene was more effici...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: White D,Chen W

    更新日期:2006-04-01 00:00:00