Stabilization of methionine-rich protein in Saccharomyces cerevisiae: targeting of BZN protein into the peroxisome.

Abstract:

:We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferase, we have significantly increased its half-life. This stabilization allowed accumulation of the BZN protein into the peroxisome as judged by cell fractionation. Accumulation of the 12-kDa protein results in a significant increase of the total methionine content in yeast cells (30%) indicating that such a microorganism could represent a practicable protected shuttle for an animal-feed additive.

journal_name

Curr Genet

journal_title

Current genetics

authors

Nicaud JM,Raynal A,Beyou A,Merkamm M,Ito H,Labat N

doi

10.1007/BF00309924

subject

Has Abstract

pub_date

1994-11-01 00:00:00

pages

390-7

issue

5-6

eissn

0172-8083

issn

1432-0983

journal_volume

26

pub_type

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