Abstract:
:We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferase, we have significantly increased its half-life. This stabilization allowed accumulation of the BZN protein into the peroxisome as judged by cell fractionation. Accumulation of the 12-kDa protein results in a significant increase of the total methionine content in yeast cells (30%) indicating that such a microorganism could represent a practicable protected shuttle for an animal-feed additive.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Nicaud JM,Raynal A,Beyou A,Merkamm M,Ito H,Labat Ndoi
10.1007/BF00309924subject
Has Abstractpub_date
1994-11-01 00:00:00pages
390-7issue
5-6eissn
0172-8083issn
1432-0983journal_volume
26pub_type
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