Genetics of carotene biosynthesis in Phycomyces.

Abstract:

:Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the different known genes and phenotypes.The gene for phytoene dehydrogenase, carB, is closely linked to carRA, a bifunctional gene specifying both lycopene cyclase and a product involved in substrate transfer. The regulatory gene carS lies on the same chromosome, but not close to the structural genes. The situation is thus reminiscent of an operon.Two chemoinsensitive mutants, showing little or no response to the effects of retinol and 2-(4-chlorophenyl)thiotriethylamine HCl on carotenogenesis, carry mutations in separate genes. One of these is probably identical with carRA; the other is a new gene termed carl.

journal_name

Curr Genet

journal_title

Current genetics

authors

Roncero MI,Cerdá-Olmedo E

doi

10.1007/BF00445733

subject

Has Abstract

pub_date

1982-05-01 00:00:00

pages

5-8

issue

1

eissn

0172-8083

issn

1432-0983

journal_volume

5

pub_type

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