Abstract:
:The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expression is held responsible for the lack of sensitive reporter genes to monitor gene expression in vivo, analyze subcellular protein localization or study protein-protein interactions. Here, we have tested the luciferase from the marine copepod Gaussia princeps (G-Luc) for its suitability as a sensitive bioluminescent reporter of gene expression in Chlamydomonas. We show that a Gaussia luciferase gene variant, engineered to match the codon usage in the Chlamydomonas nuclear genome, serves as a highly sensitive reporter of gene expression from both constitutive and inducible algal promoters. Its bioluminescence signal intensity greatly surpasses previously developed reporters for Chlamydomonas nuclear gene expression and reaches values high enough for utilizing the reporter as a tool to monitor responses to environmental stresses in vivo and to conduct high-throughput screenings for signaling mutants in Chlamydomonas.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Shao N,Bock Rdoi
10.1007/s00294-008-0189-7subject
Has Abstractpub_date
2008-06-01 00:00:00pages
381-8issue
6eissn
0172-8083issn
1432-0983journal_volume
53pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::Laccases are phenol-oxidizing, multicopper enzymes produced by fungi, plants, insects and bacteria. Fungal laccases are involved in ecologically important processes such as decomposition of lignocellulose (wood and plant material). In this work, in order to find out the role of fungal laccases upon wood colonisation a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0090-1
更新日期:2006-11-01 00:00:00
abstract::We report the isolation and expression profiles of a single-copy gene from the mycoparasitic fungus Trichoderma hamatum encoding a 60 S cytoplasmic ribosomal protein. The gene, named RPL36, was cloned through its nutrient-mediated expression, using mRNA differential screening. Its predicted ORF, interrupted by two int...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100192
更新日期:2001-05-01 00:00:00
abstract::The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. The infection process of Fol comprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virule...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-019-00931-9
更新日期:2019-06-01 00:00:00
abstract::Inorganic phosphate is an essential nutrient because it is required for the biosynthesis of nucleotides, phospholipids and metabolites in energy metabolism. During phosphate starvation, phosphatases play a major role in phosphate acquisition by hydrolyzing phosphorylated macromolecules. In Saccharomyces cerevisiae, PH...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0544-4
更新日期:2016-05-01 00:00:00
abstract::Blasticidin S-resistant mutants of S. cerevisiae were isolated and characterized. Resistant mutations were found to fall into two complementation groups. A single recessive nuclear gene was responsible for each group, donated as bls1 and bls2, respectively. A gene bls1 was linked to an ilv3 gene located on the right a...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00436968
更新日期:1985-01-01 00:00:00
abstract::Two cob- deletion mutants are characterized. One of them, M9410, is deleted for 911 bp of the noncoding sequences only which separate tRNAGlu and cob exon 1; it thus lacks most of the sequence encoding the 957 bp long cob leader (Bonitz et al. 1982) and some 20 bp 5' to it. The end points of this deletion coincide wit...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365624
更新日期:1985-01-01 00:00:00
abstract::A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310894
更新日期:1993-01-01 00:00:00
abstract::How group I introns originate in nuclear ribosomal (r)RNA genes is an important question in evolutionary biology. Central to this issue is the multitude of group I introns present in evolutionarily distantly related plant, fungal, and protist lineages, together with an understanding of their origin and lateral transfe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940100227
更新日期:2001-08-01 00:00:00
abstract::Small insertions are reported from the SSU rDNA of the genera Cladonia, Cladina, Stereocaulon, Pertusaria and Physcia (lichen-forming Lecanorales, Ascomycetes). The insertions range in length from 56 to 81 nucleotides, and occur at a shared position, 330 (relative to Escherichia coli), in a semi-conserved region of th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050317
更新日期:1998-02-01 00:00:00
abstract::Emetine resistant mutants have been isolated in the fungus Podospora anserina. Genetic analysis of 75 mutants has shown that resistance is controlled by at least two genes, EmR1 and EmR2. In vitro studies showed that resistance to emetine in EmR1 and EMR2 mutants was due to alterations in the protein synthesis machine...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00397640
更新日期:1982-10-01 00:00:00
abstract::Cell aging is the result of deteriorating competence in maintaining cellular homeostasis and quality control. Certain cell types are able to rejuvenate through asymmetric cell division by excluding aging factors, including damaged cellular compartments and extrachromosomal rDNA circles, from entering the daughter cell...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01133-4
更新日期:2020-11-27 00:00:00
abstract::A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313431
更新日期:1995-01-01 00:00:00
abstract::We have studied the splicing pathway leading to the synthesis of cytochrome oxidase subunit I (COX I) mRNA, by analysing the transcription pattern of several oxi3- splicing deficient mutants located in the first four introns of the gene. The four introns contain long open reading frames (ORFs) in phase with the upstre...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00389426
更新日期:1986-01-01 00:00:00
abstract::Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0635-x
更新日期:2017-05-01 00:00:00
abstract::A screening procedure for cell cycle mutants among is mutants of Physarum has been developed for unsynchronized microplasmodia. The synchronization of the microplasmodia and the ratio of pre- to post-mitotic nuclei were evaluated after a transient shift-up to the non-permissive temperature. In contrast with wild type ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00390945
更新日期:1980-04-01 00:00:00
abstract::The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336850
更新日期:1992-03-01 00:00:00
abstract::To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050494
更新日期:1999-10-01 00:00:00
abstract::Cellulases belong to the large family of glycosyl hydrolases (GHs) and are produced by a variety of bacteria and fungi. These extracellular enzymes act as endoglucanases (EGs), cellobiohydrolases or beta-glucosidases. In this paper, we describe molecular screening for EGs from the GH family 12. Using three homologous ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0290-2
更新日期:2002-05-01 00:00:00
abstract::Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0573-7
更新日期:2016-08-01 00:00:00
abstract::The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0085-y
更新日期:2006-09-01 00:00:00
abstract::The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050256
更新日期:1997-08-01 00:00:00
abstract::We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02426955
更新日期:1996-05-01 00:00:00
abstract::Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365693
更新日期:1981-11-01 00:00:00
abstract::The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0345-4
更新日期:2002-11-01 00:00:00
abstract::The Saccharomyces cerevisiae open reading frame YDL202w has been characterised in the course of the EUROFAN yeast genome analysis program. Disruption of YDL202w causes a respiratory deficient phenotype accompanied by a loss of mitochondrial DNA. This phenotype is usually found in mutants defective in mitochondrial rep...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050221
更新日期:1997-05-01 00:00:00
abstract::We describe the preparation of an in vitro translation system from heat shock-treated Aspergillus nidulans, capable of supporting efficient and faithful synthesis of proteins from natural and in vitro transcribed eukaryotic messages. In vitro synthesized prepro-alpha-factor was translocated across Aspergillus nidulans...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00434081
更新日期:1988-12-01 00:00:00
abstract::The putative gene encoding acetyl-CoA carboxylase, accA, has been isolated from Aspergillus nidulans. This single-copy gene has an open reading frame (ORF) of 6864 bp and contains two small introns near the 5'-end. A short ORF upstream of the ATG start codon has been identified in this gene by RT-PCR. Based on sequenc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050410
更新日期:1998-12-01 00:00:00
abstract::Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420319
更新日期:1985-03-01 00:00:00
abstract::Phylum Euglenozoa comprises three groups of eukaryotic microbes (kinetoplastids, diplonemids, and euglenids), the mitochondrial (mt) genomes of which exhibit radically different modes of organization and expression. Gene fragmentation is a striking feature of both euglenid and diplonemid mtDNAs. To rationalize the eme...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-011-0340-8
更新日期:2011-08-01 00:00:00
abstract::In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0209-7
更新日期:2008-10-01 00:00:00