An efficient cell-free translation system from Aspergillus nidulans and in vitro translocation of prepro-alpha-factor across Aspergillus microsomes.

abstract：:Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...

journal_title：Current genetics

authors： Maestroni L,Géli V,Coulon S

更新日期：2018-08-01 00:00:00

abstract：:Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

journal_title：Current genetics

authors： Goodwin TJ,Ormandy JE,Poulter RT

更新日期：2001-04-01 00:00:00

abstract：:In this paper we report the cloning, sequencing and functional characterization of CEN12 and an associated autonomously replicating sequence (ARS) from the budding yeast Saccharomyces cerevisiae. In the course of studying a dynamin-related gene, DNM1, we previously physically mapped the gene to chromosome 12. Genetic ...

journal_title：Current genetics

authors： Gammie AE,Rose MD

更新日期：1995-11-01 00:00:00

abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

journal_title：Current genetics

authors： Destombe C,Douglas SE

更新日期：1991-05-01 00:00:00

abstract：:A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...

journal_title：Current genetics

authors： Carbone I,Anderson JB,Kohn LM

更新日期：1995-01-01 00:00:00

abstract：:Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...

journal_title：Current genetics

authors： Tsirkas I,Dovrat D,Lei Y,Kalyva A,Lotysh D,Li Q,Aharoni A

更新日期：2020-10-06 00:00:00

abstract：:Like other microorganisms, free-living Candida albicans is mainly present in a three-dimensional multicellular structure, which is called a biofilm, rather than in a planktonic form. Candida albicans biofilms can be isolated from both abiotic and biotic surfaces at various locations within the host. As the number of a...

journal_title：Current genetics

authors： Mathé L,Van Dijck P

更新日期：2013-11-01 00:00:00

abstract：:The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...

journal_title：Current genetics

authors： Sánchez-Díaz A,Blanco MA,Jones N,Moreno S

更新日期：2001-09-01 00:00:00

abstract：:Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is responsible for the re...

journal_title：Current genetics

authors： Del Giudice L,Massardo DR,Manna F,Wolf K

更新日期：1986-01-01 00:00:00

abstract：:A cluster of three tRNA genes located on fragment Bam6a from Vicia faba chloroplast DNA has been sequenced: it contains the genes for tRNA(CAALeu), tRNA(UAALeu) and tRNA(Phe). The two tRNA(Leu) genes are separated by 443 bp and are transcribed divergently from different DNA strands. The intergenic region contains a se...

journal_title：Current genetics

authors： Bonnard G,Weil JH,Steinmetz A

更新日期：1985-01-01 00:00:00

abstract：:The mating-type ( MAT) genes from Rhynchosporium secalis were isolated using PCR-based methods. Characterisation of the MAT idiomorphs suggests that R. secalis is closely related to the discomycetes Pyrenopeziza brassicae and Tapesia yallundae in terms of sequence and MAT locus gene composition. The MAT1-2 idiomorph c...

journal_title：Current genetics

authors： Foster SJ,Fitt BD

更新日期：2003-12-01 00:00:00

abstract：:A simple method is described for demonstrating adaptation to alkylation damage in Aspergillus nidulans. One wild type, two MNNG-sensitive, and one MNNG-resistant strain all showed improvement in colony growth when challenged with MNNG following appropriate inducing pretreatments. Other alkylating agents (MMS, EMS) cou...

journal_title：Current genetics

authors： Hooley P,Shawcross SG,Strike P

更新日期：1988-11-01 00:00:00

abstract：:Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemorecept...

journal_title：Current genetics

authors： López-Farfán D,Reyes-Darias JA,Krell T

更新日期：2017-06-01 00:00:00

abstract：:Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...

journal_title：Current genetics

authors： Akashi K,Sakurai K,Hirayama J,Fukuzawa H,Ohyama K

更新日期：1996-07-31 00:00:00

abstract：:International efforts are underway that aim at determining the complete genome sequence of the social amoeba Dictyostelium discoideum. As strategy, a whole chromosome shotgun (WCS) approach was chosen and each of the six Dictyostelium chromosomes was assigned to project partners. The project is well advanced, chromoso...

journal_title：Current genetics

authors： Eichinger L

更新日期：2003-11-01 00:00:00

abstract：:In previous work, four genes involved in mating-type determination were cloned from reference strains of Pyrenopeziza brassicae; three genes, PAD1, PMT1, and PHB1 (re-named henceforth as MAT-1-1, MAT-1-4, and MAT1-3, respectively), are encoded by the MAT-1 idiomorph, and one gene, PHB2 (re-named MAT-2), by the corresp...

journal_title：Current genetics

authors： Singh G,Dyer PS,Ashby AM

更新日期：1999-11-01 00:00:00

abstract：:By using the fluorochrome 4'-6-diamidino-2-phenylindole (DAPI) to stain DNA one can follow the pattern of events affecting plastid DNA which occur in the formation and maturation of individual zygotes of the green flagellate Chlamydomonas moewusii. This species, like C. reinhardi, expresses uniparental inheritance of ...

journal_title：Current genetics

authors： Coleman AW,Maguire MJ

更新日期：1983-06-01 00:00:00

abstract：:In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...

journal_title：Current genetics

authors： Alam MA,Kelly JM

更新日期：2017-08-01 00:00:00

abstract：:The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...

journal_title：Current genetics

authors： Le Goff V,Perrot M,Begueret J

更新日期：1984-12-01 00:00:00

abstract：:ERG24 is the structural gene for the C14-sterol reductase in yeast. A lack of activity in that enzyme, mediated either by the morpholine fungicides or the insertional inactivation of ERG24, causes the accumulation of the aberrant sterol ignosterol. Cells producing this sterol are unable to grow aerobically in the rout...

journal_title：Current genetics

authors： Crowley JH,Tove S,Parks LW

更新日期：1998-08-01 00:00:00

abstract：:To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...

journal_title：Current genetics

authors： Pöggeler S

更新日期：1999-10-01 00:00:00

abstract：:The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...

journal_title：Current genetics

authors： Randall TA,Reddy CA

更新日期：1992-03-01 00:00:00

abstract：:Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

journal_title：Current genetics

authors： Zhang N,Cao L

更新日期：2017-10-01 00:00:00

abstract：:In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

journal_title：Current genetics

authors： Meissner M,Stark K,Cresnar B,Kirk DL,Schmitt R

更新日期：1999-12-01 00:00:00

abstract：:Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...

journal_title：Current genetics

authors： Tosato V,Sims J,West N,Colombin M,Bruschi CV

更新日期：2017-05-01 00:00:00

abstract：:Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construc...

journal_title：Current genetics

authors： Kuo J,Stirling F,Lau YH,Shulgina Y,Way JC,Silver PA

更新日期：2018-04-01 00:00:00

abstract：:The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, th...

journal_title：Current genetics

authors： Draper E,Dubrovskyi O,Bar EE,Stone DE

更新日期：2009-12-01 00:00:00

abstract：:Wild-type Neurospora crassa, strain Singapore, was transformed with a N. crassa metallothionein promoter/protyrosinase fusion gene. Transformants produced tyrosinase during vegetative growth, as determined by Western analyses and activity assays. This is in sharp contrast to wild-type strains, where this enzyme is onl...

journal_title：Current genetics

authors： Kupper U,Linden M,Cao KZ,Lerch K

更新日期：1990-11-01 00:00:00

abstract：:A vector was constructed for transformation of the plant pathogenic fungus Fusarium solani. The promoter 35Sp, from cauliflower mosaic virus, was fused to the bacterial gene APH(3')II, which confers resistance to the aminoglycoside antibiotic G418. Two transformation procedures were developed: one using isolated funga...

journal_title：Current genetics

authors： Marek ET,Schardl CL,Smith DA

更新日期：1989-06-01 00:00:00

abstract：:A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...

journal_title：Current genetics

authors： Crease TJ,Little TJ

更新日期：1997-01-01 00:00:00