Occurrence of nuclear-encoded tRNAIle in mitochondria of the liverwort Marchantia polymorpha.

Abstract:

:Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and identified three variant copies of the nuclear-encoded tRNAIle(AAU) gene from the liverwort. Northern analysis showed the presence of nuclear-encoded tRNAIle both in the mitochondrion and the cytosol, while both chloroplast DNA-encoded tRNAIle and nuclear-encoded tRNATyr were absent in liverwort mitochondria. These results unequivocally establish that import of nuclear tRNAIle into mitochondria indeed occurs in one of the most primitive plants, M. polymorpha.

journal_name

Curr Genet

journal_title

Current genetics

authors

Akashi K,Sakurai K,Hirayama J,Fukuzawa H,Ohyama K

doi

10.1007/s002940050118

subject

Has Abstract

pub_date

1996-07-31 00:00:00

pages

181-5

issue

2

eissn

0172-8083

issn

1432-0983

journal_volume

30

pub_type

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