The KP4 killer protein gene family.


:Killer protein 4 (KP4) is a well studied viral toxin secreted by the maize smut fungus Ustilago maydis that kills sensitive Ustilago strains as well as inhibits Fusarium and plant root growth by inhibiting calcium uptake. Numerous small, cysteine-rich proteins have been shown to play a critical role in fungal-plant-bacterial associations. The discovery of six KP4-like genes in F. verticillioides precipitated efforts to understand their function and evolutionary origin. Analysis of publicly available genomic sequence identified 31 additional KP4-like genes from a range of Ascomycota, a Basidiomycota, and the moss Physcomitrella patens. Sequence comparison and phylogenetic analysis indicate that the viral KP4 and the moss and fungal KP4-like genes evolved from a common ancestor providing evidence for lateral gene transfer between kingdoms. Six genes of the 37 total genes are predicted to encode a protein with two, non-identical KP4-like domains in tandem separated by 29-56 amino acids. The results suggest that two independent events led to the dual-domain KP4 genes present in different lineages of the Ascomycota. Understanding the nature and function of KP4-like proteins in mycotoxin-producing species like Fusarium may help to limit plant diseases and increase food safety and food production.


Curr Genet


Current genetics


Brown DW




Has Abstract


2011-02-01 00:00:00












  • Extracellular proteome of Aspergillus terreus grown on different carbon sources.

    abstract::Extracellular proteins of filamentous fungi are important for biomedical and biotechnological applications. Aspergillus terreus not only comprises an important class of organisms that have significant commercial relevance to the biotechnology industry, but also is an emerging fungal pathogen. However, no information i...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Han MJ,Kim NJ,Lee SY,Chang HN

    更新日期:2010-08-01 00:00:00

  • An electrophoretic karyotype of the cultivated mushroom--Agaricus bisporus.

    abstract::Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Lodder S,Gull K,Wood D

    更新日期:1993-12-01 00:00:00

  • Regulation of pho1-encoded acid phosphatase of Schizosaccharomyces pombe by adenine and phosphate.

    abstract::Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Schweingruber ME,Edenharter E,Zurlinden A,Stockmaier KM

    更新日期:1992-10-01 00:00:00

  • Genetics of carotene biosynthesis in Phycomyces.

    abstract::Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the differ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Roncero MI,Cerdá-Olmedo E

    更新日期:1982-05-01 00:00:00

  • Brassica nap cytoplasmic male sterility is associated with expression of a mtDNA region containing a chimeric gene similar to the pol CMS-associated orf224 gene.

    abstract::Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: L'Homme Y,Stahl RJ,Li XQ,Hameed A,Brown GG

    更新日期:1997-04-01 00:00:00

  • Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.

    abstract::The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Oulmouden A,Karst F

    更新日期:1991-01-01 00:00:00

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    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kubo K,Okada H,Shimamoto T,Kimori Y,Mizunuma M,Bi E,Ohnuki S,Ohya Y

    更新日期:2019-02-01 00:00:00

  • A new Hansenula polymorpha HAP4 homologue which contains only the N-terminal conserved domain of the protein is fully functional in Saccharomyces cerevisiae.

    abstract::In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Sybirna K,Guiard B,Li YF,Bao WG,Bolotin-Fukuhara M,Delahodde A

    更新日期:2005-03-01 00:00:00

  • Biochemical properties of bacterial reverse transcriptase-related (rvt) gene products: multimerization, protein priming, and nucleotide preference.

    abstract::Cellular reverse transcriptase-related (rvt) genes represent a novel class of reverse transcriptases (RTs), which are only distantly related to RTs of retrotransposons and retroviruses, but, similarly to telomerase RTs, are immobilized in the genome as single-copy genes. They have been preserved by natural selection t...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Yushenova IA,Arkhipova IR

    更新日期:2018-12-01 00:00:00

  • Posttranscriptional regulation of Git1p, the glycerophosphoinositol/glycerophosphocholine transporter of Saccharomyces cerevisiae.

    abstract::Glycerophosphoinositol (GroPIns) and glycerophosphocholine (GroPCho) are the products of phospholipase-B mediated deacylation of phosphatidylinositol and phosphatidylcholine, respectively. GroPIns and GroPCho are transported across the Saccharomyces cerevisiae plasma membrane into the cell via the transporter encoded ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Almaguer C,Fisher E,Patton-Vogt J

    更新日期:2006-12-01 00:00:00

  • Cloning, characterisation and regulation of the ornithine transaminase (otaA) gene of Aspergillus nidulans.

    abstract::The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

    journal_title:Current genetics

    pub_type: 杂志文章


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    更新日期:1999-03-01 00:00:00

  • A new point mutation in the nuclear gene of yeast mitochondrial RNA polymerase, RPO41, identifies a functionally important amino-acid residue in a protein region conserved among mitochondrial core enzymes.

    abstract::The core enzyme of mitochondrial RNA polymerase in yeast is homologous to those of bacteriophages T3, T7 and SP6. In previous studies the identification of the first conditional yeast mutant for this enzyme helped to identify the corresponding specificity factor and to elucidate their interaction inside mitochondria. ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Lisowsky T,Stein T,Michaelis G,Guan MX,Chen XJ,Clark-Walker GD

    更新日期:1996-11-01 00:00:00

  • RNA editing in six mitochondrial ribosomal protein genes of Didymium iridis.

    abstract::Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Hendrickson PG,Silliker ME

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  • Identification of paternal mitochondrial DNA sequences in the nucleus-cytoplasm hybrids of tetraploid and hexaploid wheat with D and D2 plasmons from Aegilops species.

    abstract::Mitochondrial (mt) DNA structures were studied in 12 nucleus-cytoplasm (NC) hybrids of tetraploid and hexaploid wheat in which nuclear genomes of the recurrent paternal wheat parents were combined with D and D2 plasmons from Aegilops species. RFLP analysis of 15 mtDNA regions indicated the presence of the paternal seq...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Tsukamoto N,Asakura N,Hattori N,Takumi S,Mori N,Nakamura C

    更新日期:2000-11-01 00:00:00

  • Sequence analysis of cDNAs for the human and bovine ATP synthase beta subunit: mitochondrial DNA genes sustain seventeen times more mutations.

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    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Wallace DC,Ye JH,Neckelmann SN,Singh G,Webster KA,Greenberg BD

    更新日期:1987-01-01 00:00:00

  • Composition of the spindle pole body of Saccharomyces cerevisiae and the proteins involved in its duplication.

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    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Helfant AH

    更新日期:2002-02-01 00:00:00

  • Volvox germline-specific genes that are putative targets of RegA repression encode chloroplast proteins.

    abstract::In Volvox carteri, regA acts as a master gene to suppress all germ cell functions in somatic cells. Its product, RegA, has features of a transcriptional repressor. Here we report cDNA sequences representing 15 nuclear genes with properties expected of RegA targets: they are expressed strongly in germ cells and in regA...

    journal_title:Current genetics

    pub_type: 杂志文章


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    更新日期:1999-12-01 00:00:00

  • Disruption of the cyclosporin synthetase gene of Tolypocladium niveum.

    abstract::Cyclosporin A is a potent and clinically-important immunosuppressive drug (SandimmunR). It is produced by the fungus Tolypocladium niveum. A transformation system for T. niveum ATCC34921 based on hygromycin selection was established. In order to obtain a T. niveum promoter, the cyclophilin gene was isolated using the ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Weber G,Leitner E

    更新日期:1994-11-01 00:00:00

  • The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c.

    abstract::The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differential...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Verdière J,Creusot F,Guarente L,Slonimski PP

    更新日期:1986-01-01 00:00:00

  • Molecular and functional characterization of a mutant allele of the mitogen-activated protein-kinase gene SLT2(MPK1) rescued from yeast autolytic mutants.

    abstract::We have further characterized the functionality of the Saccharomyces cerevisiae gene SLT2(MPK1), coding for a MAP-kinase homolog essential for cell integrity, which is involved in the Pkc1p signalling pathway. This gene was isolated on the basis of its capacity to complement the thermosensitive-autolytic, osmotic-reme...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Martín H,Castellanos MC,Cenamor R,Sánchez M,Molina M,Nombela C

    更新日期:1996-05-01 00:00:00

  • Developmental and light-dependent cues interact to establish steady-state levels of transcripts for photosynthesis-related genes (psbA, psbD, psaA and rbcL) in rice (Oryza sativa L.).

    abstract::The steady-state transcript levels for psbA, psbD, psaA and rbcL are low in dark-grown rice seedlings as compared to those grown in light. Following seed germination, they accumulate in an age-dependent manner, in dark as well as light, reaching a maximal level on the 7th or 8th day, before a slow decline sets in. But...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kapoor S,Maheshwari SC,Tyagi AK

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  • Regulation of mitochondrial transcription during the stringent response in yeast.

    abstract::In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Cantwell R,McEntee CM,Hudson AP

    更新日期:1992-03-01 00:00:00

  • Analysis of manganese-regulated gene expression in the ligninolytic basidiomycete Ceriporiopsis subvermispora.

    abstract::In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

    更新日期:2008-10-01 00:00:00

  • Saccharomyces cerevisiae strains sensitive to inorganic mercury. III. Tyrosine uptake.

    abstract::In Saccharomyces cerevisiae, the HGS2-1 allele confers sensitivities to inorganis mercury (Ono and Sakamoto 1985) and to excess fermentable sugars such as glucose (Sakamoto et al. 1985); exogenous tyrosine antagonizes both inorganic mercury and excess glucose. In this study, the inorganic mercury sensitive strain has ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ono B,Sakamoto E,Yamaguchi K

    更新日期:1987-01-01 00:00:00

  • RNA silencing in Chlamydomonas: mechanisms and tools.

    abstract::The generation of a comprehensive EST library and the sequencing of its genome set the stage for reverse genetics approaches in Chlamydomonas reinhardtii. However, these also require tools for the specific downregulation of target gene expression. Consequently, a large number of diverse constructs were developed aimed...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Schroda M

    更新日期:2006-02-01 00:00:00

  • Loss of 2 um DNA from Saccharomyces cerevisiae transformed with the chimaeric plasmid pJDB219.

    abstract::Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Dobson MJ,Futcher AB,Cox BS

    更新日期:1980-12-01 00:00:00

  • Genetic evidence for different RAD52-dependent intrachromosomal recombination pathways in Saccharomyces cerevisiae.

    abstract::Mutations in the RecA-like genes RAD51 and RAD57 reduce the frequency of gene conversion/reciprocal exchange between inverted repeats 7-fold. However, they enhance the frequency of deletions between direct repeats 5-12-fold. These induced deletions are RAD1- and RAD52-dependent. On the basis of these results it is pro...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Aguilera A

    更新日期:1995-03-01 00:00:00

  • Meiotic prophase-like pathway for cleavage-independent removal of cohesin for chromosome morphogenesis.

    abstract::Sister chromatid cohesion is essential for chromosome segregation both in mitosis and meiosis. Cohesion between two chromatids is mediated by a protein complex called cohesin. The loading and unloading of the cohesin are tightly regulated during the cell cycle. In vertebrate cells, cohesin is released from chromosomes...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Challa K,Shinohara M,Shinohara A

    更新日期:2019-08-01 00:00:00

  • Development of a sufficient and effective procedure for transformation of an oleaginous yeast, Rhodosporidium toruloides DMKU3-TK16.

    abstract::Rhodosporidium toruloides DMKU3-TK16 (TK16), a basidiomycetous yeast isolated in Thailand, can produce a large amount of oil corresponding to approximately 70 % of its dry cell weight. However, lack of a sufficient and efficient transformation method makes further genetic manipulation of this organism difficult. We he...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Tsai YY,Ohashi T,Kanazawa T,Polburee P,Misaki R,Limtong S,Fujiyama K

    更新日期:2017-05-01 00:00:00

  • Isolation and characterization of additional genes influencing resistance to various mutagens in the yeast Saccharomyces cerevisiae.

    abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Haase E,Servos J,Brendel M

    更新日期:1992-04-01 00:00:00