Abstract：
:The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild-type and mutant strains had the same sensitivity to photoactivated monofunctional psoralen (3-CPs + UVA) in meiosis-uncommitted and meiosis-committed stages. The mutant showed higher sensitivity to photoactivated bifunctional psoralen (8-MOP + UVA) during all stages of the meiotic cycle. Mutation induction by 3-CPs + UVA or 8-MOP + UVA in meiosis-committed cells revealed no significant differences between wild-type and the pso3-1 mutant. The status of the PSO3 gene has no influence on the kinetics of induction of gene conversion and crossing-over after 3-CPs + UVA treatment in meiosis-committed cells: gene conversion was blocked while recombination was induced. After treatment with 8-MOP + UVA gene conversion was also blocked in both strains while crossing-over could only be observed in meiosis-committed wild-type cells.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Pothin HS,da Silva KV,Brendel M,Henriques JAdoi
10.1007/BF00712961subject
Has Abstractpub_date
1994-01-01 00:00:00pages
19-23issue
1eissn
0172-8083issn
1432-0983journal_volume
25pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract：:In eukaryotes, genomic DNA is packaged into the nucleus together with histone proteins, forming chromatin. The fundamental repeating unit of chromatin is the nucleosome, a naturally symmetric structure that wraps DNA and is the substrate for numerous regulatory post-translational modifications. However, the biological...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-018-0910-0
更新日期：2019-04-01 00:00:00
abstract：:Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00326582
更新日期：1994-12-01 00:00:00
abstract：:cAMP-dependent protein kinases (PKA) are ubiquitous signalling molecules that mediate many extracellular signals in eukaryotes from yeast to men. Directing PKA to its substrates is an important level of control to ensure specificity of cAMP-mediated signal transduction. Unlike in yeast and fungi, in mammalian cells a ...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-002-0308-9
更新日期：2002-07-01 00:00:00
abstract：:We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351669
更新日期：1994-06-01 00:00:00
abstract：:The directed and controlled switching of mating type which occurs in homothallic forms of the yeast Saccharomyces cerevisiaee is dependent upon the presence of the wild type RAD52 gene. The RAD52 gene is also required for spontaneous mitotic and meiotic recombination. It has been observed that the two haploid mating t...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00377609
更新日期：1983-11-01 00:00:00
abstract：:Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-018-0808-x
更新日期：2018-08-01 00:00:00
abstract：:The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive tempera...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00376778
更新日期：1981-09-01 00:00:00
abstract：:The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00384611
更新日期：1987-01-01 00:00:00
abstract：:Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00365633
更新日期：1983-04-01 00:00:00
abstract：:Thirteen chromosomal-sized DNA bands of the cultivated mushroom Agaricus bisporus have been resolved using the method of clamped homogeneous electric field (CHEF) electrophoresis. Using chromosome size standards from Schizosaccharomyces pombe, Saccharomyces cerevisiae and Candida albicans, the estimated size of the ch...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351712
更新日期：1993-12-01 00:00:00
abstract：:The regulation of transcription of the class II yeast transposon Ty 1-17 has been examined using a Ty::LEU2 fusion gene. Deletion analysis has defined a 251 bp region within the coding sequence of the TY A gene which is essential for Ty transcription and is also required for diploid control of Ty expression. This down...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00365655
更新日期：1988-05-01 00:00:00
abstract：:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336744
更新日期：1993-01-01 00:00:00
abstract：:Retrotransposons are activated as organisms age, based on work from several model systems. Since these mobile DNA elements can promote genome instability, this has raised the possibility that they can contribute to loss of cellular function with age. Many questions remain to be addressed about the relationship between...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-015-0538-2
更新日期：2016-05-01 00:00:00
abstract：:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00368064
更新日期：1987-01-01 00:00:00
abstract：:To gain a deeper insight into the evolution of mating-type genes from filamentous ascomycetes, a comprehensive sequence analysis of PCR-amplified sequences corresponding to A- and a-specific mating-type sequences was undertaken. The study included nine homothallic (compatible) and eight heterothallic (incompatible) me...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050494
更新日期：1999-10-01 00:00:00
abstract：:The presence of the endochitinase CHIT36 from Trichoderma harzianum TM was assessed in several antagonistic Trichoderma strains belonging to different molecular taxonomic groups. CHIT37 from T. harzianum CECT 2413 was sequenced and found to display 89% homology with CHIT36 at the amino acid level. Northern analysis sh...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-002-0345-4
更新日期：2002-11-01 00:00:00
abstract：:Fifty isolates of Ascochyta rabiei (Pass.) Labr. were hierarchically sampled from four well-separated locations of a single chickpea field in Beja (Tunisia), and single-spored. DNA was isolated from in-vitro-grown mycelia, digested with HinfI or RsaI, and hybridized to a set of synthetic oligonucleotides complementary...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00309547
更新日期：1994-09-01 00:00:00
abstract：:The RAD52 group of genes in the yeast Saccharomyces cerevisiae controls the repair of DNA damage by a recombinational mechanism. Despite the growing evidence for physical and biochemical interactions between the proteins of this repair group, mutations in individual genes show very different effects on various types o...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940050467
更新日期：1999-08-01 00:00:00
abstract：:The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a pote...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s002940000160
更新日期：2000-12-01 00:00:00
abstract：:Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-020-01113-8
更新日期：2020-10-06 00:00:00
abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-018-0885-x
更新日期：2019-04-01 00:00:00
abstract：:The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00336781
更新日期：1993-10-01 00:00:00
abstract：:Mutants which carry a leaky UGA nonsense mutation in the fertility gene ste10 are characterized by a deformed cell morphology which resembles that described in the literature for sterile ras1- (ste5) and ral1 to ral4 mutant cells. Although frequent conjugation attempts are observed in combinations of two ste10 mutant ...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00312768
更新日期：1991-07-01 00:00:00
abstract：:Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-007-0158-6
更新日期：2007-11-01 00:00:00
abstract：:Most organisms prefer to utilize glucose as a carbon source. Accordingly, the expression of genes involved in the catabolism of other carbon sources is repressed by the presence of glucose in a process known as (carbon) catabolite repression. However, much less is known about the relationships between "poor" carbon so...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-019-00996-6
更新日期：2019-12-01 00:00:00
abstract：:In fungi, vegetative incompatibility is a conspecific non-self recognition mechanism that restricts formation of viable heterokaryons when incompatible alleles of specific het loci interact. In Podospora anserina, three non-allelic incompatibility systems have been genetically defined involving interactions between he...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/s00294-008-0227-5
更新日期：2009-02-01 00:00:00
abstract：:The gene for subunit II of cytochrome oxidase in the yeast Hansenula saturnus was previously shown to be located on a 1.7 kb HindIII-BamHI fragment of mitochondrial DNA (Lawson and Deters, accompanying paper). In this paper, we report the nucleotide sequence of a large part of this fragment, covering the coding region...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00421605
更新日期：1985-01-01 00:00:00
abstract：:The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical lo...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00445691
更新日期：1980-07-01 00:00:00
abstract：:Homologous recombination repairs discontinuities in DNA including single-strand gaps (SSGs) and double-strand breaks (DSBs). This commentary describes how the RecBCD and RecF pathways might be exchangeable for the repair of their respective DSB and SSG canonical substrates. In particular, I will discuss how the RecBCD...
journal_title：Current genetics
pub_type： 杂志文章,评审
doi：10.1007/s00294-016-0575-5
更新日期：2016-08-01 00:00:00
abstract：:Trichoderma reesei was transformed to hygromycin B resistance using a novel vector, which contains the E. coli hygromycin B phosphotransferase gene (hph) fused between promoter and terminator elements of the homologous Trichoderma pki1 (coding for pyruvate kinase) and cbh2 (coding for cellobiohydrolase II) genes, resp...
journal_title：Current genetics
pub_type： 杂志文章
doi：10.1007/BF00351679
更新日期：1994-06-01 00:00:00
