Abstract:
:Uridine diphosphoglucose is not the sole donor for trehalose synthesis in yeast cells: an ADPG-dependent trehalose synthase, has been identified in mutant strains with undetectable UDPG-dependent trehalose-6-P synthase activity. Genetic and chromatographic studies indicate that the two activities correspond to different proteins. The apparent Km for the nucleotide is similar for both enzymes, and Mg2+ is also required for both activities; however, a striking difference was observed with respect to ATP.Mg activation. This newly determined enzymatic activity in Saccharomyces clarifies previous contradictory results with mutant strains that are able to accumulate trehalose during growth yet whose UDPG-dependent trehalose synthase activity is undetectable in vitro.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Paschoalin VM,Silva JT,Panek ADdoi
10.1007/BF00393399subject
Has Abstractpub_date
1989-08-01 00:00:00pages
81-7issue
2eissn
0172-8083issn
1432-0983journal_volume
16pub_type
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