当前位置： SCI文献检索 > CURRENT GENETICS期刊下所有文献 > Physical mapping of genetic determinants on yeast mitochondrial DNA affecting the apparent size of the Var 1 polypeptide.

Physical mapping of genetic determinants on yeast mitochondrial DNA affecting the apparent size of the Var 1 polypeptide.

Abstract：

:The var1 locus on yeast mitochondria) DNA is defined by a set of alleles that affect the apparent size (Mr 40,000-44,000) of a mitochondria) translation product (var1), a protein of the mitochondria) 38S ribosomal subunit. Using petite deletion and restriction endonuclease site mapping, we have defined the physical location of all var1 alleles to a specific restriction fragment of roughly 2.1 kbp located between the antibiotic resistance loci ery1 and olil. Surprisingly, from base sequence studies of this region by Tzagoloff et al. (1980), it appears that the DNA fragment we have mapped contains little or none of the structural gene for the var1 protein since the fragment is composed primarily of long stretches of dA + dT interspersed with short clusters high in dG + dC. Nevertheless, by a type of complementation test termed zygotic gene rescue (Strausberg and Butow, 1977), we show that with petites retaining that restriction fragment and short flanking sequences, var1 polypeptide characteristic of the strain from which the petite was derived, is expressed in zygotes formed between the petite and a wild-type tester. Thus the ability of the var1 determinant to act in trans suggests that control of expression of different var1 species involves intermolecular interactions, perhaps at the level of RNA splicing. Our results are discussed in terms of several possible models for the organization and control of the var1 structural gene.

journal_name

Curr Genet

journal_title

Current genetics

authors

Vincent RD,Perlman PS,Strausberg RL,Butow RA

doi

10.1007/BF00445691

subject

Has Abstract

pub_date

1980-07-01 00:00:00

pages

27-38

issue

1

eissn

0172-8083

issn

1432-0983

journal_volume

2

pub_type

杂志文章

相关文献

CURRENT GENETICS文献大全
  • Nuclear omnipotent suppressors of premature termination codons in mitochondrial genes affect the 37S mitoribosomal subunit.

    abstract：:nam3 and R705, yeast nuclear omnipotent suppressors of mitochondrial mit- mutations, reverse the superimposed spectrum of trans-recessive splicing defects by affecting the protein composition of the small mitoribosomal subunit. Analysis of the suppressor's interaction suggests that suppression results from mutations i...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00365647

    authors： Boguta M,Mieszczak M,Zagórski W

    更新日期：1988-02-01 00:00:00

  • Complete mitochondrial genomes of two cockroaches, Blattella germanica and Periplaneta americana, and the phylogenetic position of termites.

    abstract：:The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequen...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-012-0365-7

    authors： Xiao B,Chen AH,Zhang YY,Jiang GF,Hu CC,Zhu CD

    更新日期：2012-04-01 00:00:00

  • Stabilization of methionine-rich protein in Saccharomyces cerevisiae: targeting of BZN protein into the peroxisome.

    abstract：:We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferas...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00309924

    authors： Nicaud JM,Raynal A,Beyou A,Merkamm M,Ito H,Labat N

    更新日期：1994-11-01 00:00:00

  • Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.

    abstract：:The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00324677

    authors： Harrer R,Schwank S,Schüller HJ,Schweizer E

    更新日期：1993-07-01 00:00:00

  • Double-stranded RNA in the entomopathogenic fungus metarhizium flavoviride.

    abstract：:Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050477

    authors： Martins MK,Furlaneto MC,Sosa-Gomez DR,Faria MR,Fungaro MH

    更新日期：1999-08-01 00:00:00

  • The heterogeneity of the rDNA-ITS sequence and its phylogeny in Rhizoctonia cerealis, the cause of sharp eyespot in wheat.

    abstract：:The sequence heterogeneity of the ribosomal internal transcribed spacer (ITS) region was investigated for Rhizoctonia cerealis isolates from the anastomosis group AG-DI. Although sequence variability of the ITS has been reported in a few multinucleate R. solani isolates, it has very rarely been reported in binucleate ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-013-0397-7

    authors： Li W,Sun H,Deng Y,Zhang A,Chen H

    更新日期：2014-02-01 00:00:00

  • Nucleotide sequence of the ERG12 gene of Saccharomyces cerevisiae encoding mevalonate kinase.

    abstract：:The nucleotide sequence of the ERG12 gene, encoding mevalonate kinase, from Saccharomyces cerevisiae is presented. The longest open reading frame may code for a protein containing 443 amino acids with a deduced relative molecular mass of 48,500. The analysis of the nucleotide sequence reveals a complete identity with ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00362081

    authors： Oulmouden A,Karst F

    更新日期：1991-01-01 00:00:00

  • HBP2: a new mammalian protein that complements the fission yeast MBF transcription complex.

    abstract：:The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940100241

    authors： Sánchez-Díaz A,Blanco MA,Jones N,Moreno S

    更新日期：2001-09-01 00:00:00

  • Caffeine-resistance in S. pombe: mutations in three novel caf genes increase caffeine tolerance and affect radiation sensitivity, fertility, and cell cycle.

    abstract：:Caffeine is a well known base analogue and is cytotoxic to both animal and yeast cells. There are two possible mechanisms by which yeast cells tolerate caffeine concentrations higher than normal, by mutation or by physiological adaptation. We have isolated novel caffeine-resistant mutants of S. pombe which define thre...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050233

    authors： Benko Z,Miklos I,Carr AM,Sipiczki M

    更新日期：1997-06-01 00:00:00

  • Ectomycorrhiza-mediated repression of the high-affinity ammonium importer gene AmAMT2 in Amanita muscaria.

    abstract：:A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-006-0106-x

    authors： Willmann A,Weiss M,Nehls U

    更新日期：2007-02-01 00:00:00

  • Cold-sensitive mutation in Neurospora crassa affecting the production of 17S ribosomal RNA from ribosomal precursor RNA.

    abstract：:The cold-sensitive mutant strain of Neurospora crassa, crib-1 (PJ30201) has a conditional defect in ribosome biosynthesis. At 10 °C this mutant underproduces the small (37S) ribosomal subunit. Experiments were done to study rRNA synthesis in crib-1 after a shift from the permissive (25 °C) to the nonpermissive tempera...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00376778

    authors： Russell PJ,Selker EU,Jackson JA

    更新日期：1981-09-01 00:00:00

  • Complete chloroplast genome sequence of a major economic species, Ziziphus jujuba (Rhamnaceae).

    abstract：:Ziziphus jujuba is an important woody plant with high economic and medicinal value. Here, we analyzed and characterized the complete chloroplast (cp) genome of Z. jujuba, the first member of the Rhamnaceae family for which the chloroplast genome sequence has been reported. We also built a web browser for navigating th...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-016-0612-4

    authors： Ma Q,Li S,Bi C,Hao Z,Sun C,Ye N

    更新日期：2017-02-01 00:00:00

  • Delta-9 fatty acid desaturase gene containing a carboxyl-terminal cytochrome b5 domain from the red alga Cyanidioschyzon merolae.

    abstract：:A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acid...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050323

    authors： Itoh R,Toda K,Takahashi H,Takano H,Kuroiwa T

    更新日期：1998-03-01 00:00:00

  • Isolation and characterisation of a gene encoding protein disulphide isomerase, pdiA, from Aspergillus niger.

    abstract：:Current strategies to improve the secretion of heterologous proteins from Aspergillus niger include the manipulation of chaperones and foldases specific to the endoplasmic reticulum (ER). Here we report the isolation of a gene, pdiA, encoding a putative protein disulphide isomerase (PDI) from A. niger using the Saccha...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050187

    authors： Ngiam C,Jeenes DJ,Archer DB

    更新日期：1997-02-01 00:00:00

  • Mating types of Agrocybe aegerita.

    abstract：:Sexual reproduction in Agrocybe aegerita is controlled by the tetrapolar mechanism of homogenic incompatibility. The two mating factors, A and B, are nonequivalent and exhibit functional specificity. Fourteen distinct A factors and 17 distinct B factors were identified in 17 natural isolates from Europe and Japan. ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00445743

    authors： Meinhardt F,Leslie JF

    更新日期：1982-05-01 00:00:00

  • The Tup1-Ssn6 general repressor is involved in repression of IME1 encoding a transcriptional activator of meiosis in Saccharomyces cerevisiae.

    abstract：:Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050332

    authors： Mizuno T,Nakazawa N,Remgsamrarn P,Kunoh T,Oshima Y,Harashima S

    更新日期：1998-04-01 00:00:00

  • Characterization of a partially fertile ras1-like ste10-UGA nonsense mutant of fission yeast.

    abstract：:Mutants which carry a leaky UGA nonsense mutation in the fertility gene ste10 are characterized by a deformed cell morphology which resembles that described in the literature for sterile ras1- (ste5) and ral1 to ral4 mutant cells. Although frequent conjugation attempts are observed in combinations of two ste10 mutant ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00312768

    authors： Leupold U

    更新日期：1991-07-01 00:00:00

  • A genetic analysis of glucoamylase activity in the diastatic yeast Saccharomyces cerevisiae NCYC 625.

    abstract：:The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00314873

    authors： Patel D,Evans IH,Bevan EA

    更新日期：1990-04-01 00:00:00

  • The overproducing CYP1 and the underproducing hap1 mutations are alleles of the same gene which regulates in trans the expression of the structural genes encoding iso-cytochromes c.

    abstract：:The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differential...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00418404

    authors： Verdière J,Creusot F,Guarente L,Slonimski PP

    更新日期：1986-01-01 00:00:00

  • A new gene encoding tRNA(Pro) (GGG) is present in the chloroplast genome of black pine: a compilation of 32 tRNA genes from black pine chloroplasts.

    abstract：:The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00313804

    authors： Tsudzuki J,Ito S,Tsudzuki T,Wakasugi T,Sugiura M

    更新日期：1994-08-01 00:00:00

  • Integrative and replicative genetic transformation of Aureobasidium pullulans.

    abstract：:A hybrid selectable marker for transformation was constructed by placing the promoter (TEF1p) from the gene encoding the Aureobasidium pullulans translation elongation factor 1-alpha (TEF1) adjacent to the 5' end of the Escherichia coli hygromycin B phosphotransferase gene (HPT). Plasmids containing this hybrid gene (...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00313195

    authors： Thornewell SJ,Peery RB,Skatrud PL

    更新日期：1995-12-01 00:00:00

  • The endpoints of an inversion in wheat chloroplast DNA are associated with short repeated sequences containing homology to att-lambda.

    abstract：:The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00636479

    authors： Howe CJ

    更新日期：1985-01-01 00:00:00

  • Molecular cloning of the PEL1 gene of Saccharomyces cerevisiae that is essential for the viability of petite mutants.

    abstract：:The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00336781

    authors： Janitor M,Subík J

    更新日期：1993-10-01 00:00:00

  • Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability.

    abstract：:Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-020-01113-8

    authors： Tsirkas I,Dovrat D,Lei Y,Kalyva A,Lotysh D,Li Q,Aharoni A

    更新日期：2020-10-06 00:00:00

  • Variety is the spice of life: how to explore a redox-dependent heterogeneity in genomically identical cellular populations.

    abstract：:Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-018-0878-9

    authors： Radzinski M,Reichmann D

    更新日期：2019-02-01 00:00:00

  • Conversion of homothallic yeast to heterothallism through HO gene disruption.

    abstract：:A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothall...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00310889

    authors： van Zyl WH,Lodolo EJ,Gericke M

    更新日期：1993-01-01 00:00:00

  • Diverse roles of Dpb2, the non-catalytic subunit of DNA polymerase ε.

    abstract：:Timely progression of living cells through the cell cycle is precisely regulated. This involves a series of phosphorylation events which are regulated by various cyclins, activated in coordination with the cell cycle progression. Phosphorylated proteins govern cell growth, division as well as duplication of the geneti...

    journal_title：Current genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s00294-017-0706-7

    authors： Dmowski M,Fijałkowska IJ

    更新日期：2017-12-01 00:00:00

  • Carbon source regulation of a dextranase gene from the filamentous fungus Penicillium minioluteum.

    abstract：:The regulation of dextranase (dexA) gene expression in the filamentous fungus Penicillium minioluteum grown on different carbon sources was studied. Growth in the presence of dextran leads to high expression of the dextranase enzyme, but growth in starch, glucose, glycerol, lactose and sorbitol did not. Dextran induce...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940000119

    authors： García B,Rodríguez E

    更新日期：2000-06-01 00:00:00

  • Isolation and genetical and biochemical characterization of mutants resistant to the alkaloid lycorine.

    abstract：:Mutants resistant to 200 micrograms/ml of the alkaloid lycorine (LYCR) in non-fermentable substrate were isolated after nitrosoguanidine mutagenesis. Tetrad analysis and growth of heterozygous (LYCR/lycS) diploids from two different mutants revealed that a single nuclear and dominant mutation is responsible for the re...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00420614

    authors： Del Giudice L,Massardo DR,Manna F,Wolf K

    更新日期：1986-01-01 00:00:00

  • Isolation, sequencing, and characterization of crp-5, a gene encoding a Neurospora ribosomal protein.

    abstract：:A Neurospora crassa cytoplasmic ribosomal protein gene, crp-5, has been isolated and characterized. The cDNA was isolated by a differential screening of a cDNA library for glucose-inducible genes. The cDNA was subsequently used to identify and isolate crp-5 genomic sequences. Computer analysis of the DNA sequences sho...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00310894

    authors： Wang Z,Tarawneh KA,Free SJ

    更新日期：1993-01-01 00:00:00