当前位置： SCI文献检索 > CURRENT GENETICS期刊下所有文献 > The Tup1-Ssn6 general repressor is involved in repression of IME1 encoding a transcriptional activator of meiosis in Saccharomyces cerevisiae.

The Tup1-Ssn6 general repressor is involved in repression of IME1 encoding a transcriptional activator of meiosis in Saccharomyces cerevisiae.

Abstract：

:Ime1 plays a pivotal role in the initiation of meiosis in a/alpha diploid cells of Saccharomyces cerevisiae. In the absence of glucose and nitrogen, IME1 expression is greater in a/alpha cells than in either a or alpha cells and therefore only a/alpha, but not a/a or alpha/alpha, cells are committed to sporulation. It is known that IME1 expression is positively regulated by Mck1, Rim1, Ime4 and the Swi-Snf complex but other factors may also be involved. In addition, Rme1 is assumed to repress IME1 expression. To provide more details of the repression of expression of IME1, we have isolated mutants in which the IME1p-PHO5 fusion gene integrated at the ura3 locus is expressed in alpha cells under nutritionally rich conditions. We found that mutations occurred in TUP1, SSN6, SIN4 and RGR1, among which TUP1 and SSN6 were identified for the first time as negative regulators of IME1 expression. Deletion of the Rme1-binding site from the IME1 promoter did not result in activation of the expression of IME1 under nutritionally rich conditions, suggesting that Rme1 does not function as a DNA-binding protein with the Tup1-Ssn6 repression complex. We also demonstrated that the 294-bp fragment from nucleotide position -914 to -621 and the 301-bp fragment from nucleotide position -1215 to -915 of the IME1 promoter region contain elements acting as URS and UAS in TUP1+ and tup1 mutant cells, respectively. These findings indicate that IME1 is negatively regulated by the Tup1-Ssn6 repressor complex through two distinct upstream regions in conjunction with unidentified DNA-binding proteins.

journal_name

Curr Genet

journal_title

Current genetics

authors

Mizuno T,Nakazawa N,Remgsamrarn P,Kunoh T,Oshima Y,Harashima S

doi

10.1007/s002940050332

subject

Has Abstract

pub_date

1998-04-01 00:00:00

pages

239-47

issue

4

eissn

0172-8083

issn

1432-0983

journal_volume

33

pub_type

杂志文章

相关文献

CURRENT GENETICS文献大全
  • Comparison of ribosomal DNA ITS regions among geographic isolates of Cenococcum geophilum.

    abstract：:Cenococcum geophilum is an ecologically important mycorrhizal fungus with a global distribution and a wide host range. It has been difficult to study since it forms only sterile mycelia and, occasionally, sclerotial bodies. Because of its lack of morphological variability, its taxonomy and phylogenetic origins have un...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050449

    authors： Shinohara ML,LoBuglio KF,Rogers SO

    更新日期：1999-06-01 00:00:00

  • Using functional genomics to decipher the complexity of microbial pathogenicity.

    abstract：:From the first identification of bacteria as a causative agent of disease, researchers have been developing methods and techniques to understand their pathogenic processes. For decades, this work has been limited to looking at a small number of genetically manipulatable isolates in in vitro assays or animal models of ...

    journal_title：Current genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s00294-016-0576-4

    authors： Laabei M,Massey R

    更新日期：2016-08-01 00:00:00

  • Cloning and characterization of the nuclear gene encoding plastid glyceraldehyde-3-phosphate dehydrogenase from the marine red alga Gracilaria verrucosa.

    abstract：:The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 b...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00326308

    authors： Zhou YH,Ragan MA

    更新日期：1994-07-01 00:00:00

  • Comparison of the lignin-degrading white rot fungi Phanerochaete chrysosporium and Sporotrichum pulverulentum at the DNA level.

    abstract：:DNA-hybridisation studies showed a close relationship between Phanerochaete chrysosporium ME446, most used in lignin degradation studies, and Sporotrichum pulverulentum Novobranova, the other standard lignin degrading strain. Two other strains of P. chrysosporium were both less related. We show that P. chrysosporium M...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00410436

    authors： Raeder U,Broda P

    更新日期：1984-09-01 00:00:00

  • A mutated swi4 gene causes duplications in the mating-type region of Schizosaccharomyces pombe.

    abstract：:Efficient mating-type (MT) switching in homothallic strains of Schizosaccharomyces pombe is significantly reduced if they have a mutation in any of the eleven known swi genes. The swi4 mutation causes heterothallic as well as homothallic segregants, both of which have duplications in the MT region. In contrast to homo...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00327020

    authors： Fleck O,Heim L,Gutz H

    更新日期：1990-12-01 00:00:00

  • The Hansenula polymorpha per6 mutant is affected in two adjacent genes which encode dihydroxyacetone kinase and a novel protein, Pak1p, involved in peroxisome integrity.

    abstract：:The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyd...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050360

    authors： van der Klei IJ,van der Heide M,Baerends RJ,Rechinger KB,Nicolay K,Kiel JA,Veenhuis M

    更新日期：1998-07-01 00:00:00

  • Cloning, characterisation and regulation of the ornithine transaminase (otaA) gene of Aspergillus nidulans.

    abstract：:The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050440

    authors： Dzikowska A,Swianiewicz M,Talarczyk A,Wisniewska M,Goras M,Scazzocchio C,Weglenski P

    更新日期：1999-03-01 00:00:00

  • Import of tRNAs and aminoacyl-tRNA synthetases into mitochondria.

    abstract：:During evolution, most of the bacterial genes from the ancestral endosymbiotic alpha-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. A crucial evolutionary step was the establishment of macromolecule import systems to allow the come back of proteins and RNAs int...

    journal_title：Current genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s00294-008-0223-9

    authors： Duchêne AM,Pujol C,Maréchal-Drouard L

    更新日期：2009-02-01 00:00:00

  • Mutations at three different nuclear loci of Chlamydomonas suppress a defect in chloroplast psbD mRNA accumulation.

    abstract：:In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050020

    authors： Nickelsen J

    更新日期：2000-02-01 00:00:00

  • Chloroplast DNA from the fern Osmunda cinnamomea: physical organization, gene localization and comparison to angiosperm.

    abstract：:Chloroplast DNA from the fern Osmunda einnamomea was isolated by a sucrose gradient procedure utilizing PEG to stabilize chloroplasts. Analysis with the restriction endonucleases PvuII, Sacl and BstEII indicates a chloroplast genome size of 144 kb. A physical map of the fragments produced by these three enzymes was co...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00391801

    authors： Palmer JD,Stein DB

    更新日期：1982-08-01 00:00:00

  • Analysis of manganese-regulated gene expression in the ligninolytic basidiomycete Ceriporiopsis subvermispora.

    abstract：:In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-008-0209-7

    authors： Gutiérrez M,Rojas LA,Mancilla-Villalobos R,Seelenfreund D,Vicuña R,Lobos S

    更新日期：2008-10-01 00:00:00

  • Location and nucleotide sequence of two tRNA genes and a tRNA pseudo-gene in the maize mitochondrial genome: evidence for the transcription of a chloroplast gene in mitochondria.

    abstract：:We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00387771

    authors： Wintz H,Grienenberger JM,Weil JH,Lonsdale DM

    更新日期：1988-03-01 00:00:00

  • The influence of the Ustilago maydis REC1 gene on plasmid-chromosome recombination.

    abstract：:The REC1 gene of U. maydis has an important but ill-defined role in DNA recombination and repair. We have examined its role in plasmid-chromosome recombination. Plasmid DNA was linearized at various locations with respect to the cloned U. maydis PYR3 gene and introduced into cells by transformation. Chromosomal integr...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00326414

    authors： Banks GR,Kanuga N,Ealing J,Spanos A,Holden DW

    更新日期：1992-12-01 00:00:00

  • Altered ribosomal proteins in emetine resistant strains in the fungus Podospora anserina.

    abstract：:Emetine resistant mutants have been isolated in the fungus Podospora anserina. Genetic analysis of 75 mutants has shown that resistance is controlled by at least two genes, EmR1 and EmR2. In vitro studies showed that resistance to emetine in EmR1 and EMR2 mutants was due to alterations in the protein synthesis machine...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00397640

    authors： Crouzet M,Bégueret J

    更新日期：1982-10-01 00:00:00

  • Analysis of mitochondrial RNA in Saccharomyces cerevisiae.

    abstract：:Mitochondrial RNA from grande yeast was analyzed by electrophoresis on agarose-urea, acrylamide-urea, and methyl mercuric hydroxide-agarose gels. These gel systems display more than 40 RNA bands that copurify with mitochondria; these bands are not present in cytoplasmic RNA preparations. Analysis of molecular weight o...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00446962

    authors： Locker J,Morimoto R,Synenki RM,Rabinowitz M

    更新日期：1980-02-01 00:00:00

  • Regulation of mitochondrial transcription during the stringent response in yeast.

    abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00336848

    authors： Cantwell R,McEntee CM,Hudson AP

    更新日期：1992-03-01 00:00:00

  • Isolation and sequence of HSP30, a yeast heat-shock gene coding for a hydrophobic membrane protein.

    abstract：:Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00312631

    authors： Régnacq M,Boucherie H

    更新日期：1993-05-01 00:00:00

  • A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis: genome size, map locations of rRNA and tRNA genes, terminal inversion repeat, and restriction site polymorphism.

    abstract：:A fine restriction map of the linear mitochondrial DNA of Tetrahymena pyriformis strain ST is presented. 1. Based on agarose gel electrophoresis data together with limited nucleotide sequences available on some restriction fragments, we estimate the actual size of this genome to be about 55,000 base pairs. 2. Seven tR...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00434053

    authors： Suyama Y,Fukuhara H,Sor F

    更新日期：1985-01-01 00:00:00

  • Debaryomyces hansenii, a highly osmo-tolerant and halo-tolerant yeast, maintains activated Dhog1p in the cytoplasm during its growth under severe osmotic stress.

    abstract：:The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...

    journal_title：Current genetics

    pub_type： 杂志文章,评审

    doi：10.1007/s00294-005-0010-9

    authors： Sharma P,Meena N,Aggarwal M,Mondal AK

    更新日期：2005-09-01 00:00:00

  • A direct study of the relative synthesis of petite and grande mitochondrial DNA in zygotes from crosses involving suppressive petite mutants of Saccharomyces cerevisiae.

    abstract：:Work in recent years has produced indirect evidence to support the view that the phenomenon of suppressiveness in yeast is the result of the ability of the petite mtDNA to out-replicate the wild-type genome. We have developed a method, based on fluorography of gels containing restriction fragments of radioactively lab...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00418122

    authors： Chambers P,Gingold E

    更新日期：1986-01-01 00:00:00

  • Small insertions at a shared position in the SSU rDNA of lecanorales (lichen-forming ascomycetes).

    abstract：:Small insertions are reported from the SSU rDNA of the genera Cladonia, Cladina, Stereocaulon, Pertusaria and Physcia (lichen-forming Lecanorales, Ascomycetes). The insertions range in length from 56 to 81 nucleotides, and occur at a shared position, 330 (relative to Escherichia coli), in a semi-conserved region of th...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050317

    authors： Stenroos S,DePriest PT

    更新日期：1998-02-01 00:00:00

  • Two biochemically and genetically different forms of L dsRNA of Saccharomyces cerevisiae exist: One form, L2, is correlated.

    abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00365682

    authors： El-Sherbeini M,Bevan EA,Mitchell DJ

    更新日期：1983-03-01 00:00:00

  • Intra-specific and inter-specific conservation of mating-type genes from the discomycete plant-pathogenic fungi Pyrenopeziza brassicae and Tapesia yallundae.

    abstract：:In previous work, four genes involved in mating-type determination were cloned from reference strains of Pyrenopeziza brassicae; three genes, PAD1, PMT1, and PHB1 (re-named henceforth as MAT-1-1, MAT-1-4, and MAT1-3, respectively), are encoded by the MAT-1 idiomorph, and one gene, PHB2 (re-named MAT-2), by the corresp...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050503

    authors： Singh G,Dyer PS,Ashby AM

    更新日期：1999-11-01 00:00:00

  • Identification of extragenic suppressors of the cif1 mutation in Saccharomyces cerevisiae.

    abstract：:The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00309531

    authors： Blázquez MA,Gancedo C

    更新日期：1994-02-01 00:00:00

  • Gene overexpression screen for chromosome instability in yeast primarily identifies cell cycle progression genes.

    abstract：:Loss of heterozygosity (LOH) in a vegetatively growing diploid cell signals irregularity of mitosis. Therefore, assays of LOH serve to discover pathways critical for proper replication and segregation of chromosomes. We screened for enhanced LOH in a whole-genome collection of diploid yeast strains in which a single g...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-018-0885-x

    authors： Tutaj H,Pogoda E,Tomala K,Korona R

    更新日期：2019-04-01 00:00:00

  • A putative ABC transporter gene, ZRA1, is required for zearalenone production in Gibberella zeae.

    abstract：:Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-011-0352-4

    authors： Lee S,Son H,Lee J,Lee YR,Lee YW

    更新日期：2011-10-01 00:00:00

  • Mutation of a putative AMPK phosphorylation site abolishes the repressor activity but not the nuclear targeting of the fungal glucose regulator CRE1.

    abstract：:In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940050535

    authors： Vautard-Mey G,Fèvre M

    更新日期：2000-05-01 00:00:00

  • Unusually compact ribosomal RNA gene cluster in Sphaeronaemella fimicola.

    abstract：:The ribosomal DNA repeat unit of Sphaeronaemella fimicola was found to be a 13.7-kb tandem repeat with a relatively long nontranscribed spacer (NTS) and an unusually compact ribosomal RNA gene cluster. The DNA sequence of an 850-bp PCR amplification product containing the 3' end of the small subunit rRNA (SSrRNA) gene...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/BF00310899

    authors： Hausner G,Klassen GR,Reid J

    更新日期：1993-01-01 00:00:00

  • L1-like non-LTR retrotransposons in the yeast Candida albicans.

    abstract：:Non-LTR retrotransposons (also known as LINEs) have had a major influence on the structure of many eukaryote genomes. They are abundant in many multicellular eukaryotes, including mammals, but appear to be absent from the yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe. This absence has, to date, preclud...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s002940000181

    authors： Goodwin TJ,Ormandy JE,Poulter RT

    更新日期：2001-04-01 00:00:00

  • Fusarium oxysporum f. sp. lycopersici C2H2 transcription factor FolCzf1 is required for conidiation, fusaric acid production, and early host infection.

    abstract：:The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. The infection process of Fol comprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virule...

    journal_title：Current genetics

    pub_type： 杂志文章

    doi：10.1007/s00294-019-00931-9

    authors： Yun Y,Zhou X,Yang S,Wen Y,You H,Zheng Y,Norvienyeku J,Shim WB,Wang Z

    更新日期：2019-06-01 00:00:00