Abstract:
:The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transported to the nucleus that results in the transcription of target genes. The HOG pathway appears to be ubiquitous in yeast. Components of HOG pathway have also been identified in Debaryomyces hansenii, a highly osmotolerant and halotolerant yeast. We have studied activation of HOG pathway in D. hansenii under different stress conditions. Our experiments demonstrated that the pathway is activated by high osmolarity, oxidative and UV stress but not by heat stress. We have provided evidence, for the first time, that D. hansenii maintains phosphorylated Dhog1p in the cytoplasm during its growth under severe osmotic stress.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Sharma P,Meena N,Aggarwal M,Mondal AKdoi
10.1007/s00294-005-0010-9subject
Has Abstractpub_date
2005-09-01 00:00:00pages
162-70issue
3eissn
0172-8083issn
1432-0983journal_volume
48pub_type
杂志文章,评审相关文献
CURRENT GENETICS文献大全abstract::The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00636479
更新日期:1985-01-01 00:00:00
abstract::Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0098-6
更新日期:2006-12-01 00:00:00
abstract::Cellular heterogeneity is a widespread phenomenon, existing across organisms and serving a crucial role in evolution and cell survival. Genetically identical cells may as a result present in a variety of forms with different gene and protein expressions, as well as oxidation level. As a result, a wide range of methodo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0878-9
更新日期:2019-02-01 00:00:00
abstract::The occupancy of nucleosomes governs access to the eukaryotic genomes and results from a combination of biophysical features and the effect of ATP-dependent remodelling complexes. Most promoter regions show a conserved pattern characterized by a nucleosome-depleted region (NDR) flanked by nucleosomal arrays. The conse...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0642-y
更新日期:2017-05-01 00:00:00
abstract::For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0073-2
更新日期:2006-07-01 00:00:00
abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351744
更新日期:1992-07-01 00:00:00
abstract::Previous work in other laboratories has shown that several mutagen sensitive mutants of Neurospora crassa are extremely sensitive to low levels of histidine in the culture medium. We have shown that wild type Neurospora accumulates nicks or breaks in the DNA in the presence of histidine. The number of nicks accumulati...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365660
更新日期:1988-05-01 00:00:00
abstract::Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420319
更新日期:1985-03-01 00:00:00
abstract::When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00712973
更新日期:1994-01-01 00:00:00
abstract::Two plasmids containing Saccharomyces cerevisiae 2 µm DNA sequences and the S. cerevisiae LEU2 gene have been found to display incompatibility with 2 µm DNA; in the presence of the LEU2 plasmids, 2 µm DNA can be lost. The LEU2 plasmids can be lost spontaneously after (and before) 2 µm DNA loss has occurred, so that st...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00435686
更新日期:1980-12-01 00:00:00
abstract::In Chlamydomonas reinhardtii, one displacement loop region which initiates the replication of chloroplast DNA was located on a 1.05 kb restriction fragment. This fragment was cloned and sequenced. In this report, the galK expression plasmid, pKO1 was used to screen for the presence of any prokaryotic promoter within t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00418528
更新日期:1986-01-01 00:00:00
abstract::Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351689
更新日期:1992-04-01 00:00:00
abstract::Somatic fusion between cells of Chlamydomonas containing complementing cell wall and auxotrophic mutations and bearing chloroplast markers for resistance to antibiotics (streptomycin or spectinomycin) have been performed to analyze the mode of chloroplast gene transmission in the fusion products. Prototrophic colonies...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00419578
更新日期:1981-04-01 00:00:00
abstract::Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-020-01053-3
更新日期:2020-08-01 00:00:00
abstract::Emetine resistant mutants have been isolated in the fungus Podospora anserina. Genetic analysis of 75 mutants has shown that resistance is controlled by at least two genes, EmR1 and EmR2. In vitro studies showed that resistance to emetine in EmR1 and EMR2 mutants was due to alterations in the protein synthesis machine...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00397640
更新日期:1982-10-01 00:00:00
abstract::Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310500
更新日期:1994-10-01 00:00:00
abstract::Cells divide with appropriate frequency by coupling division to growth-that is, cells divide only when they have grown sufficiently large. This process is poorly understood, but has been studied using cell size mutants. In principle, mutations affecting cell size could affect the mean size ("set-point" mutants), or th...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01098-4
更新日期:2020-11-05 00:00:00
abstract::The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940000130
更新日期:2000-07-01 00:00:00
abstract::Chloroplast genes are typically organized into polycistronic transcription units that give rise to complex sets of mono- and oligo-cistronic overlapping RNAs through a series of processing steps. The psbB operon contains genes for the PSII (psbB, psbT, psbH) and cytochrome b(6)f (petB and petD) complexes which are nee...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050487
更新日期:1999-09-01 00:00:00
abstract::A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypept...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310816
更新日期:1995-10-01 00:00:00
abstract::A 1,380-bp intervening sequence within the mitochondrial small subunit ribosomal RNA (mt SSU rRNA) gene of the fungus Sclerotinia sclerotiorum has been sequenced and identified as a group-I intron. This is the first report of an intron in the mt SSU rRNA gene. The intron shows close similarity in secondary structure t...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00313431
更新日期:1995-01-01 00:00:00
abstract::A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the c...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351723
更新日期:1992-09-01 00:00:00
abstract::Mycorrhizal ascomycetes are ecologically and commercially important fungi that have proved impervious to genetic transformation so far. We report here on the successful transient transformation of Tuber borchii, an ectomycorrhizal ascomycete that colonizes a variety of trees and produces highly prized hypogeous fruitb...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-005-0579-z
更新日期:2005-07-01 00:00:00
abstract::By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00419729
更新日期:1984-05-01 00:00:00
abstract::Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0545-3
更新日期:2016-05-01 00:00:00
abstract::Ubiquitination/deubiquitination pathways are now recognized as key components of gene regulatory mechanisms in eukaryotes. The major transcriptional repressor for carbon catabolite repression in Aspergillus nidulans is CreA, and mutational analysis led to the suggestion that a regulatory ubiquitination/deubiquitinatio...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0643-x
更新日期:2016-09-02 00:00:00
abstract::Glutathione (GSH) is an abundantly produced low-molecular-weight (LMW) thiol in many organisms. However, a number of Gram-positive bacteria do not produce GSH, but instead produce bacillithiol (BSH) as one of the major LMW thiols. Similar to GSH, studies have found that BSH has various roles in the cell, including pro...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-015-0511-0
更新日期:2016-02-01 00:00:00
abstract::Mitochondrial genomes in organisms from diverse phylogenetic groups vary in both size and molecular form. Although the types of mitochondrial genome appear very dissimilar, several lines of evidence argue that they do not differ radically. This would imply that interconversion between different types of mitochondrial ...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-003-0426-z
更新日期:2003-11-01 00:00:00
abstract::An exceptional mode of mtDNA inheritance involving separate maternal and paternal transmission routes has been reported recently in the mussel Mytilus edulis. This mode of inheritance provides an explanation for the high levels of heteroplasmy for two highly diverged genomes observed in males of this species. Here we ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02221509
更新日期:1996-04-01 00:00:00
abstract::We have investigated chromosomal segregation during meiosis in a cross between two polymorphic haploid laboratory strains of Saccharomyces cerevisiae, FL100 and GRF18. These two strains have large chromosome-length polymorphisms for chromosomes I and III allowing for easy scoring of parental chromosomes after meiotic ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050124
更新日期:1996-08-01 00:00:00