Abstract:
:A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypeptide. pph-1 is located between pdx-1 and col-4 on the right arm of N.crassa linkage group IV. pph-1 transcript levels are highest during the first hours of conidial germination. Failure to obtain viable progeny in which pph-1 had been inactivated via the repeat-induced point (RIP) mutation process, and evidence that nuclei harboring a disrupted pph-1 gene could only be maintained in a heterokaryon, indicated that a functional pph-1 gene is essential for fungal growth. This is the first report providing evidence that inactivation of a single-type-2A protein phosphatase gene results in a lethal phenotype in fungi.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Yatzkan E,Yarden Odoi
10.1007/BF00310816subject
Has Abstractpub_date
1995-10-01 00:00:00pages
458-66issue
5eissn
0172-8083issn
1432-0983journal_volume
28pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00712961
更新日期:1994-01-01 00:00:00
abstract::Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365693
更新日期:1981-11-01 00:00:00
abstract::A strain dependent growth on lactate in the presence of antimycin A (AA) has been observed - the strain D261 can grow on lactate and AA, whereas in the strain K8/6C antimycin A prevents the utilization of lactate and the induction of LDH.Genetic analysis demonstrates that growth on lactate in the presence of AA segreg...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00433906
更新日期:1984-08-01 00:00:00
abstract::Adaptation by natural selection might improve the fitness of an organism and its probability to survive in unfavorable environmental conditions. Decoding the genetic basis of adaptive evolution is one of the great challenges to deal with. To this purpose, Saccharomyces cerevisiae has been largely investigated because ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-016-0635-x
更新日期:2017-05-01 00:00:00
abstract::The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0426-1
更新日期:2014-08-01 00:00:00
abstract::The HOG pathway is an important mitogen-activated protein kinase (MAPK) signal transduction pathway in Saccharomyces cerevisiae that mediates adaptation of cells to hyper-osmotic stress. Activation of this pathway causes rapid but transient, phosphorylation of the MAPK Hog1p. Phosphorylated Hog1p is rapidly transporte...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-005-0010-9
更新日期:2005-09-01 00:00:00
abstract::Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-007-0155-9
更新日期:2008-01-01 00:00:00
abstract::The Cercospora nicotianae SOR1 gene is required for resistance to singlet oxygen-generating photosensitizers. SOR1 was characterized in the wild-type and in five photosensitizer-sensitive mutant strains which are complemented to photosensitizer resistance by transformation with SOR1. Sequence analysis determined that ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050423
更新日期:1999-01-01 00:00:00
abstract::In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050204
更新日期:1997-03-01 00:00:00
abstract::By using mitochondrial DNA (mtDNA) restriction fragment length polymorphisms (RFLPs) as genetic markers, the modes of mitochondrial inheritance in sexual crosses and protoplast cell fusions of the higher basidiomycete Lentinula edodes were examined. All newly established dikaryons from reciprocal crosses between compa...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00314446
更新日期:1995-05-01 00:00:00
abstract::Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050247
更新日期:1997-07-01 00:00:00
abstract::In biological systems, reactive oxygen species (ROS) represent 'double edged swords': as signaling molecules they are essential for proper development, as reactive agents they cause molecular damage and adverse effects like degeneration and aging. A well-coordinated control of ROS is therefore of key importance. Super...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0446-x
更新日期:2015-02-01 00:00:00
abstract::The single-copy nuclear gene (GapA), encoding the plastid-localized glyceraldehyde-3-phosphate dehydrogenase (GAPDH) of the marine red alga Gracilaria verrucosa, has been cloned and sequenced. The GapA transcriptional initiation site was located 49 bp upstream of the start codon, and a putative TATA box was found 54 b...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326308
更新日期:1994-07-01 00:00:00
abstract::The mitochondrial (mt) genome of the sea anemone Metridium senile contains genes for only two transfer RNAs (tRNAs), tRNAf-Met and tRNATrp. Experiments were conducted to seek evidence for the occurrence of functional tRNAs corresponding to these genes and for the participation of nuclear DNA-encoded tRNAs in mt-protei...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-013-0395-9
更新日期:2013-08-01 00:00:00
abstract::Two genetically different isolates of the homothallic Oomycete, Phytophthora sojae, were demonstrated to outcross and form hybrid oospores after co-culturing in vitro. Random amplified polymorphic DNA (RAPD) markers revealed ten hybrids among 354 oospores analysed. One F1 hybrid was allowed to self fertilise and produ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326582
更新日期:1994-12-01 00:00:00
abstract::The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336850
更新日期:1992-03-01 00:00:00
abstract::Two different cytoplasmic male-sterility (CMS) systems, nap and pol, are found in the oilseed rape (canola) species Brassica napus. Physical mapping studies have previously shown that organizational differences between the sterile pol and fertile cam mitochondrial genomes are restricted to a relatively small region im...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050212
更新日期:1997-04-01 00:00:00
abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-020-01118-3
更新日期:2020-11-04 00:00:00
abstract::Fusarium virguliforme is a soil-borne pathogenic fungus that causes sudden death syndrome (SDS) in soybean. Its pathogenicity is believed to require the activity of cell-wall-degrading enzymes (CWDEs). The sucrose non-fermenting protein kinase 1 gene (SNF1) is a key component of the glucose de-repression pathway in ye...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-017-0676-9
更新日期:2017-08-01 00:00:00
abstract::Mitochondrial DNA (mtDNA) repair occurs in all eukaryotic organisms and is essential for the maintenance of mitochondrial function. Evidence from both humans and yeast suggests that mismatch repair is one of the pathways that functions in overall mtDNA stability. In the mitochondria of the yeast Saccharomyces cerevisi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0537-1
更新日期:2005-02-01 00:00:00
abstract::The endpoints of an inversion in wheat chloroplast DNA are shown to be associated with copies of a short repeated sequence. Recombination across the repeats in an inverted configuration may have been responsible for the inversion, although they are currently in a direct orientation owing to a second inversion. The rep...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00636479
更新日期:1985-01-01 00:00:00
abstract::Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0808-x
更新日期:2018-08-01 00:00:00
abstract::Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-011-0352-4
更新日期:2011-10-01 00:00:00
abstract::RAPD markers have been obtained in Cladosporium fulvum, in order to establish a genetic map based on mitotic recombination in this imperfect fungus. Segregation analysis has provided molecular evidence of a high degree of recombination during the parasexual cycle, and of the ploidy level of the parasexual progeny. A m...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351783
更新日期:1994-05-01 00:00:00
abstract::Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00798749
更新日期:1985-01-01 00:00:00
abstract::Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-020-01150-3
更新日期:2021-01-06 00:00:00
abstract::A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00310893
更新日期:1993-01-01 00:00:00
abstract::To supply abundant ribosomes, multiple copies of ribosomal RNA genes (rDNA) are conserved from bacterial to human cells. In eukaryotic genomes, clusters of tandemly repeated rDNA units are present, and their number is stably maintained. Due to high level of transcription of rRNA genes, the repetitive structure is pron...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00956-0
更新日期:2019-08-01 00:00:00
abstract::The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351854
更新日期:1993-11-01 00:00:00
abstract::We have determined the size, the restriction map and the gene order of the mitochondrial genome of the yeast Saccharomyces uvarum. Sequence analysis of the mitochondrial COXII gene confirmed the position of this yeast in the Saccharomyces cerevisiae-like group, near Saccharomyces cerevisiae and Saccharomyces douglasii...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050308
更新日期:1998-01-01 00:00:00