Abstract:
:Protoplasts of a pAN7-1-transformed isolate of Cladosporium fulvum race 4, harbouring a tandem duplication of the vector, and an untransformed race 5 isolate were fused, without selection for the presence of vector sequences. Fusion products were allowed to haploidize spontaneously. The inheritance of pAN7-1 sequences and the expression of the hph gene was studied in 85 progeny. A very high proportion (80%) of the progeny contained vector sequences and 70% of the progeny were resistant to hygromycin. Inactivation of the hph gene occurred in eight progeny. Rearrangement of vector sequences can account for the inactivation: there is no evidence for any RIP-like mechanism. A number of novel bands were observed.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Arnau J,Oliver RPdoi
10.1007/BF00312643subject
Has Abstractpub_date
1993-05-01 00:00:00pages
508-11issue
5-6eissn
0172-8083issn
1432-0983journal_volume
23pub_type
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