Abstract:
:Chemoreceptor-based signaling is a major bacterial signal transduction mechanism. Escherichia coli, the traditional model, has five chemoreceptors. Recent genome analyses have shown that many bacteria have a much higher number of chemoreceptors. Pseudomonas putida KT2440 is an alternative model that has 27 chemoreceptors and the cognate chemoeffector is known for many of them. Here, we address the question whether and which factors modulate chemoreceptor gene expression. We report reverse transcriptase quantitative PCR measurements of all KT2440 chemoreceptor genes. Transcript levels of individual chemoreceptors differed largely, namely up to 174-fold between the most and least abundant. The cognate chemoeffectors had three different effects on the expression of their chemoreceptor genes. In some cases, the respective chemoeffectors, shown previously to be C- and/or N-sources, induced the expression. In contrast, for the two inorganic phosphate sensing chemoreceptors, the chemoeffector caused dramatic reduction in expression. For four other receptors, including the three TCA cycle intermediate sensing receptors, the chemoeffector did not cause any significant alterations. In addition, a significant number of receptors were induced in minimal growth medium and in the stationary phase. We show here that environmental cues determine largely chemoreceptor expression. This work will serve as reference for analogous studies in other bacteria.
journal_name
Curr Genetjournal_title
Current geneticsauthors
López-Farfán D,Reyes-Darias JA,Krell Tdoi
10.1007/s00294-016-0646-7subject
Has Abstractpub_date
2017-06-01 00:00:00pages
457-470issue
3eissn
0172-8083issn
1432-0983pii
10.1007/s00294-016-0646-7journal_volume
63pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::In this work, we explore the use of the unbiased cDNA-AFLP strategy to identify genes involved in Mn(2+) homeostasis in Ceriporiopsis subvermispora. In this ligninolytic white-rot fungus, whose genome has not yet been sequenced, three Mn peroxidase genes responding to Mn(2+) have been characterized. Using cDNA-AFLP to...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-008-0209-7
更新日期:2008-10-01 00:00:00
abstract::Previous work in other laboratories has shown that several mutagen sensitive mutants of Neurospora crassa are extremely sensitive to low levels of histidine in the culture medium. We have shown that wild type Neurospora accumulates nicks or breaks in the DNA in the presence of histidine. The number of nicks accumulati...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365660
更新日期:1988-05-01 00:00:00
abstract::La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312776
更新日期:1991-07-01 00:00:00
abstract::The complete nucleotide sequence of one of three linear DNA plasmids (pRS64-2) from the plant pathogenic fungus Rhizoctonia solani was determined. The pRS64-2 DNA consisted of 2877 nucleotides. The nucleotide sequences of the middle 2.2-kb regions of the other two plasmids (pRS64-1 and pRS64-3) were also determined. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050299
更新日期:1997-12-01 00:00:00
abstract::This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312615
更新日期:1990-03-01 00:00:00
abstract::The previous ovservation that in dividing sister cells of Schizosaccharomyces pombe only one of two parallel divisions can be accompanied by a switch of mating type, herein termed "Miyata's rule", has been confirmed in pedigrees of diploid cells heterozygous for the mat2-Pm-B102 allele. Moreover, this rule appears to ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00417817
更新日期:1984-04-01 00:00:00
abstract::Six ultra violet (UV) mutageneses were performed on the spo76 UV-sensitive mutant of Sordaria macrospora. Spo76 shows an early centromere cleavage associated with an arrest at the first meiotic division and therefore does not form ascospores. Moreover, it exhibits altered pairing structure (synaptonemal complex), reve...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00447389
更新日期:1986-01-01 00:00:00
abstract::Mixed aeciospore isolates of Cronartium flaccidum and Peridermium pini were obtained from single-tree infections in Britain, Italy and Greece. The 5.8s ribosomal RNA gene and flanking intergenic transcribed spacer regions ITS1 and ITS2 were found to be highly similar between C. flaccidum and P. pini. Within samples he...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF02208620
更新日期:1996-03-01 00:00:00
abstract::Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-016-0573-7
更新日期:2016-08-01 00:00:00
abstract::The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336781
更新日期:1993-10-01 00:00:00
abstract::The zinc finger transcription factor Crz1 is an important downstream regulator of calcium-dependent signal transduction pathways in many organisms. The function of Crz1 in the wheat-head blight pathogen Fusarium graminearum remains unclear. In this study, we identified and functionally characterised FgCrz1A, a potenti...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-018-0853-5
更新日期:2019-02-01 00:00:00
abstract::Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-002-0316-9
更新日期:2002-08-01 00:00:00
abstract::In Chlamydomonas reinhardtii, the expression of the nit1 gene encoding nitrate reductase is dependent on the nature of the nitrogen source and on other environmental factors. We have fused the nit1 promoter region to the arylsulphatase (ars) reporter gene lacking its own promoter and introduced this chimeric construct...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050204
更新日期:1997-03-01 00:00:00
abstract::Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-020-01053-3
更新日期:2020-08-01 00:00:00
abstract::Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-004-0558-9
更新日期:2005-03-01 00:00:00
abstract::Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050477
更新日期:1999-08-01 00:00:00
abstract::In the photosynthetic chloroplast mutant PRB2A of Chlamydomonas reinhardtii the psbD mRNA is unstable. Three strains were isolated, in which the underlying site-directed mutation within the psbD 5' UTR (untranslated region) is suppressed. In all three suppressors, psbD RNA levels and RNA 5' maturation are restored to ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050020
更新日期:2000-02-01 00:00:00
abstract::Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050118
更新日期:1996-07-31 00:00:00
abstract::Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00445879
更新日期:1983-09-01 00:00:00
abstract::The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420621
更新日期:1980-10-01 00:00:00
abstract::The physical positions of the DNA sequences encoding the five consecutive enzyme activities required to metabolise 3-deoxy-D-arabino-heptulosonic acid-7-phosphate to 5-enolpyruvyl-shikimate-3phosphate, which are encoded by the A. nidulans Arom polypeptide have been determined. Subfragments of the Arom locus encoding E...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384611
更新日期:1987-01-01 00:00:00
abstract::Selection of mutants of Saccharomyces cerevisiae sensitive to the DNA cross-linking agent nitrogen mustard (HN2) at two temperatures (23 °C and 36 °C) yielded two isolates with thermoconditionally enhanced (ts) sensitivity to the mutagen. Both were due to single recessive nuclear genes. Mutant allele snm1-2 (ts) showe...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365693
更新日期:1981-11-01 00:00:00
abstract::In order to develop an eukaryotic vector with the Podospora plasmid, further characterization is required of the mitochondrial DNA into which this plasmid is integrated, a physical map (restriction sites) of the Podospora chondriome (size 95 kb) has been completed. As prerequisite for the establishment of a genetic (f...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00365705
更新日期:1982-07-01 00:00:00
abstract::The nature of extra-chromosomal maintenance of the transforming plasmid p12-6 in Phanerochaete chrysosporium was studied. Our results indicate that the plasmid is maintained in the fungal transformants extra-chromosomally as part of a larger endogenous plasmid (designated pME) of P. chrysosporium. Using the total DNA ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00336850
更新日期:1992-03-01 00:00:00
abstract::Using differential hybridization, a gene preferentially expressed during entry into stationary phase has been isolated. Subsequent analysis indicated that this gene corresponds to a heat-shock gene. The nucleotide sequence has been determined. It revealed a 332 amino-acid protein. No similarities to any previously kno...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312631
更新日期:1993-05-01 00:00:00
abstract::The microtubule-organizing center (MTOC) is the organelle that manages the distribution of microtubule filaments in the cell. It includes such centers of microtubule organization as basal bodies, nucleus-associated bodies, spindle pole bodies and centrosomes. The centrosome is the MTOC in most animal cells. Despite th...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-001-0263-x
更新日期:2002-02-01 00:00:00
abstract::Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050247
更新日期:1997-07-01 00:00:00
abstract::The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00324677
更新日期:1993-07-01 00:00:00
abstract::The insect pathogenic fungus Metarhizium anisopliae is widely used as an insect biocontrol agent. The M. anisopliae conidium plays an important role in pathogenesis and disease transmission. The aim of this study was to identify genes whose expression is up-regulated during conidiogenous cell development. This is a po...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-009-0242-1
更新日期:2009-06-01 00:00:00
abstract::Mitochondrial DNA from etiolated seedling of male-fertile (F) and male-sterile (S) sunflower (Helianthus annuus L.) has been investigated for the first time in an isogenic couple HA89. 1. The mt-DNAs isolated from the S and F cytoplasms give a sharp and symmetrical band at 1.706 g/ml in an analytical CsCI gradient ult...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420319
更新日期:1985-03-01 00:00:00