Characterization of thermosensitive mutants of Physarum polycephalum a plasmodial screening method for cell cycle mutants based upon synchronization at the restrictive temperature.

abstract：:In Saccharomyces cerevisiae, the HAP transcriptional complex is involved in the fermentation-respiration shift. This complex is composed of four subunits. Three subunits are necessary for DNA-binding, whereas the Hap4p subunit, glucose-repressed, contains the transcriptional activation domain. Hap4p is the key regulat...

journal_title：Current genetics

authors： Sybirna K,Guiard B,Li YF,Bao WG,Bolotin-Fukuhara M,Delahodde A

更新日期：2005-03-01 00:00:00

abstract：:Genetic analysis of carotenogenesis in Phycomyces is hampered by the inability of most mutants to complete the sexual cycle. Heterokaryons between complementing mutants or between a mutant and a helper strain are, however, fertile. Using this method crosses have been carried out between mutants representing the differ...

journal_title：Current genetics

authors： Roncero MI,Cerdá-Olmedo E

更新日期：1982-05-01 00:00:00

abstract：:A homologous transformation system for Aspergillus oryzae is described. The system is based on an A. oryzae strain deficient in orotidine-5'-phosphate decarboxylase (pyrG) and the vector pAO4-2, which contains a functional A. oryzae pyrG gene as selection marker. Transformation of the A. oryzae pyrG mutant with circul...

journal_title：Current genetics

authors： de Ruiter-Jacobs YM,Broekhuijsen M,Unkles SE,Campbell EI,Kinghorn JR,Contreras R,Pouwels PH,van den Hondel CA

更新日期：1989-09-01 00:00:00

abstract：:The sequence heterogeneity of the ribosomal internal transcribed spacer (ITS) region was investigated for Rhizoctonia cerealis isolates from the anastomosis group AG-DI. Although sequence variability of the ITS has been reported in a few multinucleate R. solani isolates, it has very rarely been reported in binucleate ...

journal_title：Current genetics

authors： Li W,Sun H,Deng Y,Zhang A,Chen H

更新日期：2014-02-01 00:00:00

abstract：:The mitochondrial genomes are one of the most information-rich markers in phylogenetics. The relationships within superorder Dictyoptera have been debated in the literature. However, the closely related termites (Isoptera) are retained as unranked taxon within the order Blattaria (cockroaches). In this work, we sequen...

journal_title：Current genetics

authors： Xiao B,Chen AH,Zhang YY,Jiang GF,Hu CC,Zhu CD

更新日期：2012-04-01 00:00:00

abstract：:The mammalian HBP2 gene has been isolated by functional complementation of cells unable to undergo DNA replication in fission yeast. HBP2 is a protein with a high mobility group (HMG) domain that belongs to the Sox (Sry-related HMG box) family of transcription factors. As in other members of the family, the HMG box in...

journal_title：Current genetics

authors： Sánchez-Díaz A,Blanco MA,Jones N,Moreno S

更新日期：2001-09-01 00:00:00

abstract：:Seven MboI fragments spanning the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae strain D273-10B were cloned in the BamHI site of the integrative yeast vector YIp5 and the capacity for autonomous replication was subsequently assayed in yeast. The positive correlation found between the ars-like activity...

journal_title：Current genetics

authors： Delouya D,Bonjardim CA,Nobrega FG

更新日期：1987-01-01 00:00:00

abstract：:Over the last decade, mutation studies have grown in popularity due to the affordability and accessibility of whole genome sequencing. As the number of species in which spontaneous mutation has been directly estimated approaches 20 across two domains of life, questions arise over the repeatability of results in such e...

journal_title：Current genetics

authors： Behringer MG,Hall DW

更新日期：2016-08-01 00:00:00

abstract：:Laccases are phenol-oxidizing, multicopper enzymes produced by fungi, plants, insects and bacteria. Fungal laccases are involved in ecologically important processes such as decomposition of lignocellulose (wood and plant material). In this work, in order to find out the role of fungal laccases upon wood colonisation a...

journal_title：Current genetics

authors： Mäkelä MR,Hildén KS,Hakala TK,Hatakka A,Lundell TK

更新日期：2006-11-01 00:00:00

abstract：:Efficient mating-type (MT) switching in homothallic strains of Schizosaccharomyces pombe is significantly reduced if they have a mutation in any of the eleven known swi genes. The swi4 mutation causes heterothallic as well as homothallic segregants, both of which have duplications in the MT region. In contrast to homo...

journal_title：Current genetics

authors： Fleck O,Heim L,Gutz H

更新日期：1990-12-01 00:00:00

abstract：:Studies from this laboratory have suggested that mitochondrial (mt) transcription in yeast (Saccharomyces cerevisiae) is governed by changing cellular cAMP levels, and that the mechanism of such transcriptional regulation requires cAMP-dependent protein kinase (PKA) activity; these observations, in turn, suggest a tra...

journal_title：Current genetics

authors： Iqbal J,Gérard HC,Rahman MU,Hudson AP

更新日期：1996-12-01 00:00:00

abstract：:We have sequenced a region (7,376-bp) of the mitochondrial (mt) DNA (54 kb) of the cellular slime mold, Dictyostelium discoideum. From the DNA and amino-acid sequence comparisons with known sequences, genes for ATPase subunit 9 (ATP9), cytochrome b (CYTB), NADH dehydrogenase subunits 1, 3 and 6 (ND1, ND3 and ND6), sma...

journal_title：Current genetics

authors： Angata K,Kuroe K,Yanagisawa K,Tanaka Y

更新日期：1995-02-01 00:00:00

abstract：:The hybrid vector pCP2, consisting of the bacterial plasmid pBR325, the nuclear gene Leu-2 of Saccharomyces cerevisiae and a fragment of mitochondrial DNA from Cephalosporium acremonium, was found to associate with the nucleus in a transformed strain of Saccharomyces cerevisiae. This was inducted by (1) efficient expr...

journal_title：Current genetics

authors： Tudzynski P,Esser K

更新日期：1983-04-01 00:00:00

abstract：:A molecular clone containing the complete sequence of a mitochondrial circular plasmid-like DNA (the R plasmid) isolated from the date-palm variety V3DP was used as a probe in Southern analyses of mitochondrial DNA prepared from other varieties. Another circular structure (the S plasmid) was detected in some of these ...

journal_title：Current genetics

authors： Benslimane AA,Hartmann C,Ouenzar B,Rode A

更新日期：1996-05-01 00:00:00

abstract：:The CYP1 gene has previously been identified as coding for a positive trans active factor that activates the expression of CYC1 and CYP3, which are the structural genes for isol1- and iso2-cytochrome c. Two phenotypically distinct classes of CYP1 mutations can be obtained indicating that CYC1 and CYP3 are differential...

journal_title：Current genetics

authors： Verdière J,Creusot F,Guarente L,Slonimski PP

更新日期：1986-01-01 00:00:00

abstract：:In yeast (S. cerevisiae) the stringent response is known to include rapid, selective, and severe transcriptional curtailment for genes specifying cytoplasmic rRNAs and r-proteins. We have shown that transcription of the mitochondrial 21S rRNA gene is also congruently and selectively curtailed during the yeast stringen...

journal_title：Current genetics

authors： Cantwell R,McEntee CM,Hudson AP

更新日期：1992-03-01 00:00:00

abstract：:Somatic fusion between cells of Chlamydomonas containing complementing cell wall and auxotrophic mutations and bearing chloroplast markers for resistance to antibiotics (streptomycin or spectinomycin) have been performed to analyze the mode of chloroplast gene transmission in the fusion products. Prototrophic colonies...

journal_title：Current genetics

authors： Matagne RF

更新日期：1981-04-01 00:00:00

abstract：:Nucleic acid extracted from killer strains of Saccharomyces cerevisiae and run on agarose gels under sub-optimal conditions for M dsRNA yielded two species of L dsRNA (L1 and L2) which migrated at slightly different rates. L2 dsRNA was shown to be dependent upon MAK3, MAK10 and PES1 but not MAK1 nuclear genes for its ...

journal_title：Current genetics

authors： El-Sherbeini M,Bevan EA,Mitchell DJ

更新日期：1983-03-01 00:00:00

abstract：:The regulation of dextranase (dexA) gene expression in the filamentous fungus Penicillium minioluteum grown on different carbon sources was studied. Growth in the presence of dextran leads to high expression of the dextranase enzyme, but growth in starch, glucose, glycerol, lactose and sorbitol did not. Dextran induce...

journal_title：Current genetics

authors： García B,Rodríguez E

更新日期：2000-06-01 00:00:00

abstract：:A cDNA library made from Euglena polyadenylated RNA has been constructed in the expression vector lambda gt11. The library was screened with antibodies directed against the LHCP apoproteins. Several putative LHCP clones were isolated and analyzed. We present the nucleotide and derived amino-acid sequence of an 800 bp ...

journal_title：Current genetics

authors： Houlne G,Schantz R

更新日期：1987-01-01 00:00:00

abstract：:We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...

journal_title：Current genetics

authors： Wintz H,Grienenberger JM,Weil JH,Lonsdale DM

更新日期：1988-03-01 00:00:00

abstract：:A system for the expression of an ATP binding cassette (ABC) transporter from the soybean pathogen Phytophthora sojae is described. Pdr1, an ABC transporter with homology to the pleiotropic drug resistance (PDR) family of transporters, was cloned by primer walking from a P. sojae genomic library. Reverse transcriptase...

journal_title：Current genetics

authors： Connolly MS,Sakihama Y,Phuntumart V,Jiang Y,Warren F,Mourant L,Morris PF

更新日期：2005-12-01 00:00:00

abstract：:O-Mannosylation is suggested to be essential for protein secretion in Trichoderma reesei. In protein O-glycosylation, the first mannosyl residue is transferred to a serine or threonine hydroxyl group of the protein from dolichyl phosphate mannose by protein O-mannosyltransferase. In Saccharomyces cerevisiae, seven PMT...

journal_title：Current genetics

authors： Zakrzewska A,Migdalski A,Saloheimo M,Penttila ME,Palamarczyk G,Kruszewska JS

更新日期：2003-04-01 00:00:00

abstract：:Many species are able to hybridize, but the sterility of these hybrids effectively prevents gene flow between the species, reproductively isolating them and allowing them to evolve independently. Yeast hybrids formed by Saccharomyces cerevisiae and Saccharomyces paradoxus parents are viable and able to grow by mitosis...

journal_title：Current genetics

authors： Ono J,Greig D

更新日期：2020-06-01 00:00:00

abstract：:The soil-borne, asexual fungus Fusarium oxysporum f.sp. lycopersici (Fol) is a causal agent of tomato wilt disease. The infection process of Fol comprises root recognition, adhesion, penetration, colonization of the root cortex and hyphal proliferation within the xylem vessels, which are under the regulation of virule...

journal_title：Current genetics

authors： Yun Y,Zhou X,Yang S,Wen Y,You H,Zheng Y,Norvienyeku J,Shim WB,Wang Z

更新日期：2019-06-01 00:00:00

abstract：:By functional complementation of the auxotrophic requirements for choline of a cdg1, cho2 double-mutant, by transformation with a genomic DNA library in a high copy number plasmid, two different types of complementing DNA inserts were identified. One type of insert was earlier shown to represent the CHO2 structural ge...

journal_title：Current genetics

authors： Preitschopf W,Lückl H,Summers E,Henry SA,Paltauf F,Kohlwein SD

更新日期：1993-02-01 00:00:00

abstract：:A gene of Puccinia graminis f. sp. tritici, coding for the translation elongation factor 1 alpha (EF-1 alpha), was isolated from a P. graminis genomic library using the EF-1 alpha gene sequence of Absidia glauca. The coding region of 1389 nucleotides encodes a polypeptide of 463 amino acids and is interrupted by eight...

journal_title：Current genetics

authors： Schillberg S,Gross P,Tiburzy R

更新日期：1995-03-01 00:00:00

abstract：:In the red alga Gracilaria verrucosa, the genes encoding the large and the small subunit of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) are separated by a short spacer of less than 131 bp. Sequencing of PCR-amplified Rubisco spacers from a number of populations of G. verrucosa was performed to assess the...

journal_title：Current genetics

authors： Destombe C,Douglas SE

更新日期：1991-05-01 00:00:00

abstract：:Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

journal_title：Current genetics

authors： Kerkmann K,Lehming N

更新日期：2001-07-01 00:00:00

abstract：:Screening of a multi-copy vector-based yeast genomic library in haploid cells of wild-type Saccharomyces cerevisiae yielded transformants hyper-resistant to various chemical mutagens. Genetical analysis of the yeast insert DNAs revealed three genes SNG1, SNQ2, and SNQ3 that confer the phenotype hyper-resistance to MNN...

journal_title：Current genetics

authors： Haase E,Servos J,Brendel M

更新日期：1992-04-01 00:00:00