ARS activity along the yeast mitochondrial apocytochrome b region: correlation with the location of petite genomes and consensus sequences.

abstract：:Full genome recoding, or rewriting codon meaning, through chemical synthesis of entire bacterial chromosomes has become feasible in the past several years. Recoding an organism can impart new properties including non-natural amino acid incorporation, virus resistance, and biocontainment. The estimated cost of construc...

journal_title：Current genetics

authors： Kuo J,Stirling F,Lau YH,Shulgina Y,Way JC,Silver PA

更新日期：2018-04-01 00:00:00

abstract：:The S. cerevisiae strain h56 is a temperature-sensitive mit- mutant containing a single nucleotide substitution in the region 5' to the reading frame of the mitochondrial var1 gene. The mutation decreases the efficiency of processing of a precursor RNA such that little var1 mRNA is produced at the restrictive temperat...

journal_title：Current genetics

authors： Smooker PM,Macreadie IJ,Wright JL,Lukins HB

更新日期：1994-03-01 00:00:00

abstract：:The directed and controlled switching of mating type which occurs in homothallic forms of the yeast Saccharomyces cerevisiaee is dependent upon the presence of the wild type RAD52 gene. The RAD52 gene is also required for spontaneous mitotic and meiotic recombination. It has been observed that the two haploid mating t...

journal_title：Current genetics

authors： Malone RE,Hyman D

更新日期：1983-11-01 00:00:00

abstract：:The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expres...

journal_title：Current genetics

authors： Shao N,Bock R

更新日期：2008-06-01 00:00:00

abstract：:The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...

journal_title：Current genetics

authors： Harrer R,Schwank S,Schüller HJ,Schweizer E

更新日期：1993-07-01 00:00:00

abstract：:Homologous recombination repairs discontinuities in DNA including single-strand gaps (SSGs) and double-strand breaks (DSBs). This commentary describes how the RecBCD and RecF pathways might be exchangeable for the repair of their respective DSB and SSG canonical substrates. In particular, I will discuss how the RecBCD...

journal_title：Current genetics

authors： Pagès V

更新日期：2016-08-01 00:00:00

abstract：:Gene expression using alternative transcription start sites (TSSs) is an important transcriptional regulatory mechanism for environmental responses in eukaryotes. Here, we identify two alternative TSSs in the enolase-encoding gene (enoA) in Aspergillus oryzae, an industrially important filamentous fungus. TSS use in e...

journal_title：Current genetics

authors： Inoue T,Toji H,Tanaka M,Takama M,Hasegawa-Shiro S,Yamaki Y,Shintani T,Gomi K

更新日期：2020-08-01 00:00:00

abstract：:For a comprehensive evaluation of genes that have their expression modulated during exposure of the mycelia to voriconazole, we performed a large-scale analysis of gene expression in Aspergillus fumigatus using a microarray hybridization approach. By comparing the expression of genes between the reference time and aft...

journal_title：Current genetics

authors： da Silva Ferreira ME,Malavazi I,Savoldi M,Brakhage AA,Goldman MH,Kim HS,Nierman WC,Goldman GH

更新日期：2006-07-01 00:00:00

abstract：:A PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), was used for assessing genomic variability among a wide range of culture collection strains of black Aspergilli and related species. The performance of this technique is compared with that of the two other genetic techniques most comm...

journal_title：Current genetics

authors： Megnegneau B,Debets F,Hoekstra RF

更新日期：1993-01-01 00:00:00

abstract：:The chloroplast genome of black pine (Pinus thunbergii), a gymnosperm, contains 32 different tRNA genes, 30 of which correspond to those previously identified in tobacco and rice chloroplast genomes. Two additional genes encode tRNA(Pro) (GGG) and tRNA(Arg) (CCG); the former is newly identified while the latter is pre...

journal_title：Current genetics

authors： Tsudzuki J,Ito S,Tsudzuki T,Wakasugi T,Sugiura M

更新日期：1994-08-01 00:00:00

abstract：:Mono-ubiquitinated histone H2B (H2B-Ub) is important for chromatin regulation of transcription, chromatin assembly, and also influences heterochromatin. In this review, we discuss the effects of H2B-Ub from nucleosome to higher-order chromatin structure. We then assess what is currently known of the role of H2B-Ub in ...

journal_title：Current genetics

authors： Zukowski A,Johnson AM

更新日期：2018-08-01 00:00:00

abstract：:A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothall...

journal_title：Current genetics

authors： van Zyl WH,Lodolo EJ,Gericke M

更新日期：1993-01-01 00:00:00

abstract：:Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...

journal_title：Current genetics

authors： Maestroni L,Géli V,Coulon S

更新日期：2018-08-01 00:00:00

abstract：:NRE, the nitrogen regulatory protein of Penicillium chrysogenum, contains a single Cys2/Cys2-type zinc-finger motif followed immediately by a highly basic region. The zinc-finger domain was expressed to Escherichia coli as a fusion protein with beta-galactosidase. In order to test the putative DNA-binding ability of N...

journal_title：Current genetics

authors： Haas H,Marzluf GA

更新日期：1995-07-01 00:00:00

abstract：:Although the mitochondrial genomes of the Chinese Spring and Aquila varieties of wheat are normally similar in organization, this is not so in tissue cultures initiated from their immature embryos where the mitochondrial genomes of both are rearranged and in different, characteristic, ways. However, the mitochondrial ...

journal_title：Current genetics

authors： Hartmann C,De Buyser J,Henry Y,Morère-Le Paven MC,Dyer TA,Rode A

更新日期：1992-05-01 00:00:00

abstract：:A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transfo...

journal_title：Current genetics

authors： Keon JP,White GA,Hargreaves JA

更新日期：1991-06-01 00:00:00

abstract：:Cell fusion occurs regularly during the vegetative and sexual phases of the life cycle in filamentous fungi. Here, we present a simple and efficient method that can detect even rare hyphal fusion events. Using the homothallic ascomycete Sordaria macrospora as an experimental system, we developed a histone-assisted mer...

journal_title：Current genetics

authors： Rech C,Engh I,Kück U

更新日期：2007-11-01 00:00:00

abstract：:The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...

journal_title：Current genetics

authors： Pothin HS,da Silva KV,Brendel M,Henriques JA

更新日期：1994-01-01 00:00:00

abstract：:Inorganic phosphate is an essential nutrient because it is required for the biosynthesis of nucleotides, phospholipids and metabolites in energy metabolism. During phosphate starvation, phosphatases play a major role in phosphate acquisition by hydrolyzing phosphorylated macromolecules. In Saccharomyces cerevisiae, PH...

journal_title：Current genetics

authors： Yadav KK,Singh N,Rajasekharan R

更新日期：2016-05-01 00:00:00

abstract：:The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic ch...

journal_title：Current genetics

authors： Swinnen E,Rosseels J,Winderickx J

更新日期：2005-07-01 00:00:00

abstract：:The wild diastatic yeast Saccharomyces cerevisiae NCYC 625 has been shown to be homozygous for the glucoamylase-specifying gene STA2. spoII-1-mapping has positioned STA2 on chromosome II. Expression of STA2 is suppressed in some but not all diploids capable of sporulation, and is also inhibited by unlinked nuclear sup...

journal_title：Current genetics

authors： Patel D,Evans IH,Bevan EA

更新日期：1990-04-01 00:00:00

abstract：:By completing and correcting the sequence of a 1.8 kb DNA segment downstream of the oxi2 gene of Saccharomyces cerevisiae, a long, potentially coding sequence ("RF2") has been identified. The sequence is rather closely related to the RF1 open reading frame, downstream of the oxil gene, and, further, to the major famil...

journal_title：Current genetics

authors： Michel F

更新日期：1984-05-01 00:00:00

abstract：:The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...

journal_title：Current genetics

authors： Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina L

更新日期：1993-01-01 00:00:00

abstract：:This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...

journal_title：Current genetics

authors： Cantatore P,Roberti M,Loguercio Polosa P,Mustich A,Gadaleta MN

更新日期：1990-03-01 00:00:00

abstract：:Approximately, 1% of the genes in eukaryotic genomes encode for helicases, which make the number of helicases expressed in the cell considerably high. Helicases are motor proteins that participate in many central aspects of the nuclear and mitochondrial genomes, and based on their helicase motif conservation, they are...

journal_title：Current genetics

authors： Sabouri N

更新日期：2017-08-01 00:00:00

abstract：:The zinc finger transcription factor Crz1 is an important downstream regulator of calcium-dependent signal transduction pathways in many organisms. The function of Crz1 in the wheat-head blight pathogen Fusarium graminearum remains unclear. In this study, we identified and functionally characterised FgCrz1A, a potenti...

journal_title：Current genetics

authors： Chen L,Tong Q,Zhang C,Ding K

更新日期：2019-02-01 00:00:00

abstract：:In filamentous ascomycetes, glucose repression is mediated by CRE1, a zinc-finger protein related to Miglp from yeast. Five putative AMPK phosphorylation motifs identified in the glucose repressor from the phytopathogenic fungus Sclerotinia sclerotiorum were mutated in a GFP::CRE1 translational fusion. Complementation...

journal_title：Current genetics

authors： Vautard-Mey G,Fèvre M

更新日期：2000-05-01 00:00:00

abstract：:The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...

journal_title：Current genetics

authors： Hubert JC,Bach ML,Lacroute F

更新日期：1980-10-01 00:00:00

abstract：:Fission yeast is a simple eukaryotic model organism in which many aspects of cell cycle control can be explored. We examined by homologous recombination whether the human CDC25A phosphatase could substitute for the function of the fission yeast Cdc25. We first show: (a). that CDC25A efficiently replaces the endogenous...

journal_title：Current genetics

authors： Mondesert O,Lemaire M,Brezak MC,Galera-Contour MO,Prevost G,Ducommun B,Bugler B

更新日期：2004-05-01 00:00:00

abstract：:Most cheese-making filamentous fungi lack suitable molecular tools to improve their biotechnology potential. Penicillium roqueforti, a species of high industrial importance, would benefit from functional data yielded by molecular genetic approaches. This work provides the first example of gene replacement by homologou...

journal_title：Current genetics

authors： Goarin A,Silar P,Malagnac F

更新日期：2015-05-01 00:00:00