Isolation, characterization and sequence of a gene conferring resistance to the systemic fungicide carboxin from the maize smut pathogen, Ustilago maydis.

Abstract:

:A gene which confers resistance to the systemic fungicide carboxin (Cbx) has been isolated from the maize pathogen, Ustilago maydis, by transferring a plasmid gene library from a Cbx-resistant mutant strain into a sensitive strain and selecting for expression of the resistance gene. Five plasmids, rescued from transformants which exhibited enhanced resistance to Cbx, were shown to have DNA inserts with common restriction enzyme fragments. All the plasmids transformed a sensitive U. maydis strain to Cbx resistance. The gene (Cbxr), sub-cloned on a 3.2 kb EcoR1-HindIII fragment, transformed U. maydis to Cbx resistance at frequencies similar to those obtained with the bacterial Hygromycin B resistance (HygBr) gene. The sequence of the Cbxr gene showed a high degree of homology to succinate dehydrogenase (EC 1.3.99.1) iron-sulphur subunit genes from other organisms.

journal_name

Curr Genet

journal_title

Current genetics

authors

Keon JP,White GA,Hargreaves JA

doi

10.1007/BF00312739

subject

Has Abstract

pub_date

1991-06-01 00:00:00

pages

475-81

issue

6

eissn

0172-8083

issn

1432-0983

journal_volume

19

pub_type

杂志文章
  • Self-fertility and uni-directional mating-type switching in Ceratocystis coerulescens, a filamentous ascomycete.

    abstract::Individual perithecia from selfings of most Ceratocystis species produce both self-fertile and self-sterile progeny, apparently due to uni-directional mating-type switching. In C. coerulescens, male-only mutants of otherwise hermaphroditic and self-fertile strains were self-sterile and were used in crossings to demons...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050247

    authors: Harrington TC,McNew DL

    更新日期:1997-07-01 00:00:00

  • A DNA polymerase activity with characteristics of a reverse transcriptase in Podospora anserina.

    abstract::In the filamentous ascomycete fungus Podospora anserina, senescence is associated with dramatic changes of the juvenile mitochondrial genome. During senescence specific regions of the non-senescent genome are excised, ligated and amplified as a plasmid. In this paper, we report our studies on nuclear-mitochondrial ext...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00418411

    authors: Steinhilber W,Cummings DJ

    更新日期:1986-01-01 00:00:00

  • STEEx, a boundary between the world of quiescence and the vegetative cycle.

    abstract::Telomere maintenance mechanism is poorly studied in quiescence, a reversible non-proliferative state. We previously described in fission yeast a new mode of repair of telomeres named STEEx, that specifically operates in post-mitotic cells harboring eroded telomeres. This mechanism, promoted by transcription-induced te...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-018-0808-x

    authors: Maestroni L,Géli V,Coulon S

    更新日期:2018-08-01 00:00:00

  • Immunity factors for two related tRNAGln targeting killer toxins distinguish cognate and non-cognate toxic subunits.

    abstract::The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-014-0426-1

    authors: Klassen R,Kast A,Wünsche G,Paluszynski JP,Wemhoff S,Meinhardt F

    更新日期:2014-08-01 00:00:00

  • Dse1 may control cross talk between the pheromone and filamentation pathways in yeast.

    abstract::The filamentous/invasive growth pathway is activated by nutrient limitation in the haploid form of the yeast Saccharomyces cerevisiae, whereas exposure to mating-pheromone causes cells to differentiate into gametes. Although these two pathways respond to very different stimuli and generate very different responses, th...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-009-0274-6

    authors: Draper E,Dubrovskyi O,Bar EE,Stone DE

    更新日期:2009-12-01 00:00:00

  • Mapping and characterization of Paracentrotus lividus mitochondrial transcripts: multiple and overlapping transcription units.

    abstract::This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00312615

    authors: Cantatore P,Roberti M,Loguercio Polosa P,Mustich A,Gadaleta MN

    更新日期:1990-03-01 00:00:00

  • A putative ABC transporter gene, ZRA1, is required for zearalenone production in Gibberella zeae.

    abstract::Zearalenone (ZEA) is a secondary metabolite produced by various Fusarium species and causes estrogenic disorders in humans and animals. Recent studies have identified the ZEA biosynthesis gene cluster in F. graminearum, but other genes such as transporters responsible for ZEA export have not been identified in the clu...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-011-0352-4

    authors: Lee S,Son H,Lee J,Lee YR,Lee YW

    更新日期:2011-10-01 00:00:00

  • Differentiation of Fusarium solani f. sp. cucurbitae races 1 and 2 by random amplification of polymorphic DNA.

    abstract::We have used a PCR-based technique, involving the random amplification of polymorphic DNA (RAPD), to assess genome variability between 21 isolates from F. solani f. sp. cucurbitae races 1 and 2. Based on RAPD marker patterns the isolates fell into two distinct groups corresponding to mating populations MPI and MPV. Fo...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00317067

    authors: Crowhurst RN,Hawthorne BT,Rikkerink EH,Templeton MD

    更新日期:1991-11-01 00:00:00

  • Partial sequence of the mitochondrial genome of the crustacean Daphnia pulex.

    abstract::A 4062-nucleotide (nt) fragment of the mitochondrial genome of the crustacean Daphnia pulex was sequenced and found to contain the complete genes for eight tRNAs and five proteins (ATP6, ATP8, COII, COIII, ND3) and the partial sequence of COI. In combination with data described previously, approximately 50% of the D. ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050175

    authors: Crease TJ,Little TJ

    更新日期:1997-01-01 00:00:00

  • The isoprenoid pathway in the ectomycorrhizal fungus Tuber borchii Vittad.: cloning and characterisation of the tbhmgr, tbfpps and tbsqs genes.

    abstract::The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-006-0097-7

    authors: Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

    更新日期:2006-12-01 00:00:00

  • The transcription factor FgCrz1A is essential for fungal development, virulence, deoxynivalenol biosynthesis and stress responses in Fusarium graminearum.

    abstract::The zinc finger transcription factor Crz1 is an important downstream regulator of calcium-dependent signal transduction pathways in many organisms. The function of Crz1 in the wheat-head blight pathogen Fusarium graminearum remains unclear. In this study, we identified and functionally characterised FgCrz1A, a potenti...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-018-0853-5

    authors: Chen L,Tong Q,Zhang C,Ding K

    更新日期:2019-02-01 00:00:00

  • Comparative analysis of the region of the mitochondrial genome containing the ATPase subunit 9 gene in the two related yeast species Saccharomyces douglasii and Saccharomyces cerevisiae.

    abstract::We have determined the nucleotide sequence of a region of the mitochondrial genome of the yeast Saccharomyces douglasii which contains the ATPase subunit 9 gene and part of the intergenic sequences that surround it. The gene is 228 nucleotides long and encodes a polypeptide of 76 aa. A comparison of the coding sequenc...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00351669

    authors: Nicoletti L,Laveder P,Pellizzari R,Cardazzo B,Carignani G

    更新日期:1994-06-01 00:00:00

  • Nucleotide sequence of the mitochondrial cytochrome oxidase subunit II gene in the yeast Hansenula saturnus.

    abstract::The gene for subunit II of cytochrome oxidase in the yeast Hansenula saturnus was previously shown to be located on a 1.7 kb HindIII-BamHI fragment of mitochondrial DNA (Lawson and Deters, accompanying paper). In this paper, we report the nucleotide sequence of a large part of this fragment, covering the coding region...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00421605

    authors: Lawson JE,Deters DW

    更新日期:1985-01-01 00:00:00

  • Cointegration of transforming DNAs in Aspergillus nidulans: a model using autonomously-replicating plasmids.

    abstract::Transforming DNAs form cointegrates in Aspergillus nidulans by homologous and non-homologous recombination as well as by end-to-end ligation of linear fragments. This process has been studied by means of a model in which the linkage of a marker gene to the origin of autonomous replication AMA1 was selected for. Recomb...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00310500

    authors: Aleksenko AY

    更新日期:1994-10-01 00:00:00

  • Ubiquitin-like activating enzymes BcAtg3 and BcAtg7 participate in development and pathogenesis of Botrytis cinerea.

    abstract::In eukaryotes, the ubiquitin-like (UBL) protein-activating enzymes play a crucial role in autophagy process, however, it is poorly characterized in filamentous fungi. Here, we investigated the functions of two UBL activating enzymes, BcAtg3 (E2) and BcAtg7 (E1) in the plant pathogenic fungus Botrytis cinerea. The phys...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-018-0810-3

    authors: Ren W,Sang C,Shi D,Song X,Zhou M,Chen C

    更新日期:2018-08-01 00:00:00

  • Alkylation mutagenesis in Saccharomyces cerevisiae: lack of evidence for an adaptive response.

    abstract::We have found no evidence for an adaptive response for either lethality or mutagenesis following treatment of Saccharomyces cerevisiae with N-methyl-N'-nitro-N-nitrosoguanidine (MNNG). The rad6 and rad52 mutants of S. cerevisiae are highly defective in MNNG and ethyl methanesulfonate induced mutagenesis of both statio...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00410912

    authors: Polakowska R,Perozzi G,Prakash L

    更新日期:1986-01-01 00:00:00

  • Functional characterization of SOR1, a gene required for resistance to photosensitizing toxins in the fungus Cercospora nicotianae.

    abstract::The Cercospora nicotianae SOR1 gene is required for resistance to singlet oxygen-generating photosensitizers. SOR1 was characterized in the wild-type and in five photosensitizer-sensitive mutant strains which are complemented to photosensitizer resistance by transformation with SOR1. Sequence analysis determined that ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940050423

    authors: Ehrenshaft M,Chung KR,Jenns AE,Daub ME

    更新日期:1999-01-01 00:00:00

  • Cryptosporidium parvum Cpn60 targets a relict organelle.

    abstract::Chaperonin 60 (Cpn60) is a well-established marker protein for eukaryotic mitochondria and plastids. In order to determine whether the small double-membrane-bounded organelle posterior to the nucleus in the apicomplexan Cryptosporidium parvum is a mitochondrion, the Cpn60 gene of C. parvum sporozoites ( CpCpn60) was a...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-003-0432-1

    authors: Riordan CE,Ault JG,Langreth SG,Keithly JS

    更新日期:2003-11-01 00:00:00

  • Cloning of the mating type locus from Ascochyta lentis (teleomorph: Didymella lentis) and development of a multiplex PCR mating assay for Ascochyta species.

    abstract::The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-006-0085-y

    authors: Chérif M,Chilvers MI,Akamatsu H,Peever TL,Kaiser WJ

    更新日期:2006-09-01 00:00:00

  • Deletion of RNQ1 gene reveals novel functional relationship between divergently transcribed Bik1p/CLIP-170 and Sfi1p in spindle pole body separation.

    abstract::Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-006-0098-6

    authors: Strawn LA,True HL

    更新日期:2006-12-01 00:00:00

  • Gene fragmentation: a key to mitochondrial genome evolution in Euglenozoa?

    abstract::Phylum Euglenozoa comprises three groups of eukaryotic microbes (kinetoplastids, diplonemids, and euglenids), the mitochondrial (mt) genomes of which exhibit radically different modes of organization and expression. Gene fragmentation is a striking feature of both euglenid and diplonemid mtDNAs. To rationalize the eme...

    journal_title:Current genetics

    pub_type: 杂志文章,评审

    doi:10.1007/s00294-011-0340-8

    authors: Flegontov P,Gray MW,Burger G,Lukeš J

    更新日期:2011-08-01 00:00:00

  • Characterization of hch, the Podospora anserina homolog of the het-c heterokaryon incompatibility gene of Neurospora crassa.

    abstract::The het-c locus controls heterokaryon formation in Neurospora crassa. It is subject to balancing selection operating to maintain polymorphism at that locus in natural populations. We have isolated hch, the het-c homolog from the related species Podospora anserina (hch for het-c homolog), in order to determine if this ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940000130

    authors: Saupe SJ,Clavé C,Sabourin M,Bégueret J

    更新日期:2000-07-01 00:00:00

  • Cac1 WHD and PIP domains have distinct roles in replisome progression and genomic stability.

    abstract::Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-020-01113-8

    authors: Tsirkas I,Dovrat D,Lei Y,Kalyva A,Lotysh D,Li Q,Aharoni A

    更新日期:2020-10-06 00:00:00

  • Characterisation of the LdsRNA encoded mRNA of yeast.

    abstract::Radioactive messenger RNA (mRNA) was isolated from a Saccharomyces cerevisiae strain containing the L double stranded RNA (dsRNA) species. This mRNA was hybridised to LdsRNA isolated from the same strain. Analysis of the hybrid formed shows it to be of a similar size to the LdsRNA. It is concluded from this result tha...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00420496

    authors: Haylock RW,Bevan EA

    更新日期:1981-12-01 00:00:00

  • Endopolygalacturonase genes and enzymes from Saccharomyces cerevisiae and Kluyveromyces marxianus.

    abstract::The gene encoding endopolygalacturonase (EC 3.2.1.15) has been cloned, sequenced and expressed from three strains of Saccharomyces cerevisiae (including nonsecretors) and three strains of Kluyveromyces marxianus. Both control and coding regions showed small differences within each species, one including loss of a pote...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940000160

    authors: Jia J,Wheals A

    更新日期:2000-12-01 00:00:00

  • Genome-wide expression analysis of a Saccharomyces cerevisiae strain deleted for the Tup1p-interacting protein Cdc73p.

    abstract::Tuplp is a general corepressor in Saccharomyces cerevisiae. We performed a split-ubiquitin screen with Tup1p as bait, in order to understand how Tuplp mediates repression. Cdc73p was the only component of a holoenzyme of transcription isolated as a Tuplp-interacting protein in this screen. Expression analysis of a str...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s002940100229

    authors: Kerkmann K,Lehming N

    更新日期:2001-07-01 00:00:00

  • A codon-optimized luciferase from Gaussia princeps facilitates the in vivo monitoring of gene expression in the model alga Chlamydomonas reinhardtii.

    abstract::The unicellular green alga Chlamydomonas reinhardtii has emerged as a superb model species in plant biology. Although the alga is easily transformable, the low efficiency of transgene expression from the Chlamydomonas nuclear genome has severely hampered functional genomics research. For example, poor transgene expres...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-008-0189-7

    authors: Shao N,Bock R

    更新日期:2008-06-01 00:00:00

  • Fission yeast Cdc23 interactions with DNA replication initiation proteins.

    abstract::Schizosaccharomyces pombe Cdc23 is an essential DNA replication protein, conserved in eukaryotes and functionally homologous with Saccharomyces cerevisiae Dna43 (Mcm10). We sought evidence for interactions between Cdc23 and the MCM2-7 complex, a component of both the pre-replicative complex and the replication fork. C...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/s00294-002-0316-9

    authors: Hart EA,Bryant JA,Moore K,Aves SJ

    更新日期:2002-08-01 00:00:00

  • A termination codon is created by RNA editing in the petunia atp9 transcript.

    abstract::Analysis of the cDNA of the atp9-1 gene transcript from petunia mitochondria has revealed that ten C residues of the gene sequence are edited into U in the mRNA. Seven of these edits result in amino acid changes and one introduces a stop codon before the end of the open reading frame predicted from the gene sequence. ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00362089

    authors: Wintz H,Hanson MR

    更新日期:1991-01-01 00:00:00

  • Electrophoretical and immunological comparison of the ribosomal proteins of the fungus Podospora anserina and the yeast Saccharomyces cerevisiae.

    abstract::The ribosomal proteins of two ascomycetes Podospora anserina and Saccharomyces cerevisiae, were compared by two dimensional electrophoresis in two different gel systems and we found only five pairs of proteins which have kept homologous physico-chemical properties under these conditions. An immunological analysis was ...

    journal_title:Current genetics

    pub_type: 杂志文章

    doi:10.1007/BF00396204

    authors: Le Goff V,Perrot M,Begueret J

    更新日期:1984-12-01 00:00:00