The isoprenoid pathway in the ectomycorrhizal fungus Tuber borchii Vittad.: cloning and characterisation of the tbhmgr, tbfpps and tbsqs genes.


:The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isoprenoid biosynthesis, 3-hydroxy-3-methylglutaryl-CoA reductase, farnesyl-diphosphate synthase (FPPS) and squalene synthase (SQS), were cloned and characterised. The analyses of their nucleotide and deduced amino acid sequences led us to identify the typical domains shown in homologous proteins. By using a quantitative real-time PCR the expression pattern of the three genes was analysed in the vegetative phase and during the complex ascoma maturation process, revealing an over-expression in the mature ascomata. The enzymatic activity of the T. borchii 3-hydroxy-3-methylglutaril-CoA reductase (HMGR) was investigated with a HPLC method, confirming that the significant isoprenoid biosynthesis in ripe ascomata proceeds not only via a transcriptional activation, but also via an enzyme activity control. These findings imply that isoprenoids play a fundamental role in Tuber ascomata, particularly in the last phases of their maturation, when they could be involved in antifungal or/and antimicrobial processes and contribute to the famous flavour of the truffle ascomata.


Curr Genet


Current genetics


Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V




Has Abstract


2006-12-01 00:00:00












  • Checkpoint functions of RecQ helicases at perturbed DNA replication fork.

    abstract::DNA replication checkpoint is a cell signaling pathway that is activated in response to perturbed replication. Although it is crucial for maintaining genomic integrity and cell survival, the exact mechanism of the checkpoint signaling remains to be understood. Emerging evidence has shown that RecQ helicases, a large f...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Ahamad N,Khan S,Mahdi ATA,Xu YJ

    更新日期:2021-01-11 00:00:00

  • Molecular cloning and analysis of the nuclear gene MRP-L6 coding for a putative mitochondrial ribosomal protein from Saccharomyces cerevisiae.

    abstract::The Saccharomyces cerevisiae nuclear gene MRP-L6 was cloned by complementation of the respiratory-deficient mutant pet-ts 2523 with a library of wild-type yeast genomic DNA. The isolated gene was part of a 3.8-kb sequenced DNA fragment containing, in addition to MRP-L6, two unassigned reading frames, ORF1 and ORF2. MR...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Harrer R,Schwank S,Schüller HJ,Schweizer E

    更新日期:1993-07-01 00:00:00

  • Functional diversity in the pH signaling pathway: an overview of the pathway regulation in Neurospora crassa.

    abstract::Microorganisms have the ability to adapt and respond to different environmental conditions, whether they are stressful or not. Although the detection and/or responding mechanisms are often unknown, a large number of proteins may participate in signal transduction pathways involved in environmental stimulus to induce p...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Virgilio S,Bertolini MC

    更新日期:2018-06-01 00:00:00

  • Small insertions at a shared position in the SSU rDNA of lecanorales (lichen-forming ascomycetes).

    abstract::Small insertions are reported from the SSU rDNA of the genera Cladonia, Cladina, Stereocaulon, Pertusaria and Physcia (lichen-forming Lecanorales, Ascomycetes). The insertions range in length from 56 to 81 nucleotides, and occur at a shared position, 330 (relative to Escherichia coli), in a semi-conserved region of th...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Stenroos S,DePriest PT

    更新日期:1998-02-01 00:00:00

  • The genetic fine structure of nonsense suppressors in Schizosaccharomyces pombe : II. sup8 and sup10.

    abstract::Meiotic fine-structure maps of two efficient UGA suppressors of Schizosaccharomyces pombe which are known (sup8-e) or inferred (sup10-e) to code for two leucine tRNAs carrying the mutant anticodon U(*)CA (Kohli et al. 1979, 1980a, b; Wetzel et al. 1979; Mao et al. 1981) are presented. In both cases, the recombination ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Munz P,Dorsch-Häsler K,Leupold U

    更新日期:1983-04-01 00:00:00

  • Identification, partial sequence and genetic analysis of mlpA, a novel gene encoding a myosin-related protein in Physarum polycephalum.

    abstract::Studies of motility in Physarum polycephalum have concentrated on the well-defined actomyosin system in plasmodia. It is clear from recent genetic studies in lower eukaryotes that myosin is involved in a number of physiological processes in addition to the contractile functions previously ascribed to the classical typ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Murray M,Foxon J,Sweeney F,Orr E

    更新日期:1994-02-01 00:00:00

  • Tmt1: the first LTR-retrotransposon from a Tuber spp.

    abstract::Retrotransposons are suitable targets for developing molecular markers for population genetics studies. Transposable elements have not yet been isolated from ectomycorrhizal fungi of the genus Tuber. In this paper, we report on the isolation and characterization of Tmt1, an LTR-retrotransposon from Tuber melanosporum....

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Riccioni C,Rubini A,Belfiori B,Passeri V,Paolocci F,Arcioni S

    更新日期:2008-01-01 00:00:00

  • Primary structure and transcription patterns of RPL36, a ribosomal protein-encoding gene of the mycoparasitic fungus, Trichoderma hamatum.

    abstract::We report the isolation and expression profiles of a single-copy gene from the mycoparasitic fungus Trichoderma hamatum encoding a 60 S cytoplasmic ribosomal protein. The gene, named RPL36, was cloned through its nutrient-mediated expression, using mRNA differential screening. Its predicted ORF, interrupted by two int...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Fekete C,Posta K,Hornok L

    更新日期:2001-05-01 00:00:00

  • Delta-9 fatty acid desaturase gene containing a carboxyl-terminal cytochrome b5 domain from the red alga Cyanidioschyzon merolae.

    abstract::A delta-9 fatty acid desaturase gene, homologous to animal and fungal acyl-coenzyme A (CoA) desaturases, was isolated from the red alga Cyanidioschyzon merolae using a degenerate PCR strategy. This gene, designated as CmFAD9, has no intron. The encoded delta-9 fatty acid desaturase (CmFad9p) consists of 476 amino acid...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Itoh R,Toda K,Takahashi H,Takano H,Kuroiwa T

    更新日期:1998-03-01 00:00:00

  • Mitochondrial DNA sequences reveal close relationships between social parasitic ants and their host species.

    abstract::In the tribe Leptothoracini, the phylogenetic relationship of socially parasitic ants (Doronomyrmex kutteri, D. goesswaldi and Harpagoxenus sublaevis) and their host species Leptothorax acervorum has been controversial. Even more controversial is the relationship between the socially parasitic ant Chalepoxenus mueller...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Baur A,Chalwatzis N,Buschinger A,Zimmermann FK

    更新日期:1995-08-01 00:00:00

  • Enhanced canavanine uptake is associated with nucleotide permeability in a thymidylate auxotroph of Saccharomyces cerevisiae.

    abstract::The recovery of spontaneous canavanine-resistant mutants is reduced dramatically in a strain of Saccharomyces cerevisiae that carries a suppressed can 1-100 allele and is permeable to and auxotrophic for thymidylate. This effect does not occur in an isogenic strain that neither takes up nor requires the nucleotide. Ho...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Kohalmi SE,Kunz BA

    更新日期:1989-02-01 00:00:00

  • Fungal effectors, the double edge sword of phytopathogens.

    abstract::Phyto-pathogenic fungi can cause huge damage to crop production. During millions of years of coexistence, fungi have evolved diverse life-style to obtain nutrients from the host and to colonize upon them. They deploy various proteinaceous as well as non-proteinaceous secreted molecules commonly referred as effectors t...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Pradhan A,Ghosh S,Sahoo D,Jha G

    更新日期:2020-11-04 00:00:00

  • Molecular cloning of the PEL1 gene of Saccharomyces cerevisiae that is essential for the viability of petite mutants.

    abstract::The PEL1 gene of Saccharomyces cerevisiae is essential for the cell viability of mitochondrial petite mutants, for the ability to utilize glycerol and ethanol on synthetic medium, and for cell growth at higher temperatures. By tetrad analysis the gene was assigned to chromosome III, centromere proximal of LEU2. The PE...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Janitor M,Subík J

    更新日期:1993-10-01 00:00:00

  • Single-strand gap repair involves both RecF and RecBCD pathways.

    abstract::Homologous recombination repairs discontinuities in DNA including single-strand gaps (SSGs) and double-strand breaks (DSBs). This commentary describes how the RecBCD and RecF pathways might be exchangeable for the repair of their respective DSB and SSG canonical substrates. In particular, I will discuss how the RecBCD...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Pagès V

    更新日期:2016-08-01 00:00:00

  • Characterization and localization of mitochondrial DNA-encoded tRNAs and nuclear DNA-encoded tRNAs in the sea anemone Metridium senile.

    abstract::The mitochondrial (mt) genome of the sea anemone Metridium senile contains genes for only two transfer RNAs (tRNAs), tRNAf-Met and tRNATrp. Experiments were conducted to seek evidence for the occurrence of functional tRNAs corresponding to these genes and for the participation of nuclear DNA-encoded tRNAs in mt-protei...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Beagley CT,Wolstenholme DR

    更新日期:2013-08-01 00:00:00

  • Identification of extragenic suppressors of the cif1 mutation in Saccharomyces cerevisiae.

    abstract::The cif1 mutation of Saccharomyces cerevisiae causes inability to grow on glucose and related fermentable carbon sources. We have isolated two different suppressor mutations that allow growth on glucose of yeasts carrying the cif1 mutation. One of them, sci1-1, is recessive and caused inability to grow on non-fermenta...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Blázquez MA,Gancedo C

    更新日期:1994-02-01 00:00:00

  • Polymorphisms in DNA of Coprinus cinereus.

    abstract::Two types of DNA sequence polymorphisms were found among different geographic isolates of the basidiomycete fungus Coprinus einereus. These strains showed gains and losses of restriction enzyme recognition sites as well as extensive insertion/ deletion variation within DNA sequences present in a single copy per haploi...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Wu MM,Cassidy JR,Pukkila PJ

    更新日期:1983-09-01 00:00:00

  • The minimum domain of Pho81 is not sufficient to control the Pho85-Rim15 effector branch involved in phosphate starvation-induced stress responses.

    abstract::The phosphate regulatory mechanism in yeast, known as the PHO pathway, is regulated by inorganic phosphate to control the expression of genes involved in the acquisition of phosphate from the medium. This pathway is also reported to contribute to other nutritional responses and as such it affects several phenotypic ch...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Swinnen E,Rosseels J,Winderickx J

    更新日期:2005-07-01 00:00:00

  • The beta-tubulin genes of Drosophila auraria are arranged in a cluster.

    abstract::When the beta 1-, beta 2- and beta 3-tubulin-specific DNAs from Drosophila melanogaster were used as probes to recognize tubulin-specific sequences in the chromosomes of Drosophila auraria, they were found to hybridize to the same polytene band in region 32C of the 2L polytene chromosome. Three overlapping clones were...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Scouras ZG,Milioni D,Yiangou M,Duchêne M,Domdey H

    更新日期:1994-01-01 00:00:00

  • Characterization and prevalence of a circular mitochondrial plasmid in senescence-prone isolates of Neurospora intermedia.

    abstract::Genetic and molecular analyses of the phenomenon of senescence-i.e., irreversible loss of growth and reproductive potential upon subculturing-in Neurospora intermedia strain M1991-60A, collected from Maddur in southern India, showed the presence of plasmid pMaddur1, which is homologous to the senescence-inducing circu...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: D'Souza AD,Sultana S,Maheshwari R

    更新日期:2005-03-01 00:00:00

  • An approach to yeast classification by mapping mitochondrial DNA from Dekkera/Brettanomyces and Eeniella genera.

    abstract::Sequences hybridizing to mitochondrial DNA probes from Saccharomyces cerevisiae have been mapped in six mitochondrial genomes from the Dekkera/Brettanomyces yeasts and in mtDNA from the closely related Eeniella nana. Sequence order for the 34.5 kbp mtDNA of E. nana is identical to that for mtDNAs from B. custersianus ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Hoeben P,Clark-Walker GD

    更新日期:1986-01-01 00:00:00

  • Evolutionary conservation of genomic sequences related to the GGP1 gene encoding a yeast GPI-anchored glycoprotein.

    abstract::The GGP1 gene encodes the only GPI-anchored glycoprotein (gp115) that has been purified to-date in the budding yeast Saccharomyces cerevisiae. It is a single-copy gene whose deduced amino-acid sequence shares no significant homology to any other known protein. In this paper we report a Southern hybridization analysis ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Vai M,Lacanà E,Gatti E,Breviario D,Popolo L,Alberghina L

    更新日期:1993-01-01 00:00:00

  • Chromosome loading of cohesin depends on conserved residues in Scc3.

    abstract::Cohesin is essential for sister chromatid cohesion, which ensures equal segregation of the chromatids to daughter cells. However, the molecular mechanism by which cohesin mediates this function is elusive. Scc3, one of the four core subunits of cohesin, is vital to cohesin activity. However, the mechanism by which Scc...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Pathania A,Liu W,Matityahu A,Irudayaraj J,Onn I

    更新日期:2021-01-06 00:00:00

  • Ubiquitin-like activating enzymes BcAtg3 and BcAtg7 participate in development and pathogenesis of Botrytis cinerea.

    abstract::In eukaryotes, the ubiquitin-like (UBL) protein-activating enzymes play a crucial role in autophagy process, however, it is poorly characterized in filamentous fungi. Here, we investigated the functions of two UBL activating enzymes, BcAtg3 (E2) and BcAtg7 (E1) in the plant pathogenic fungus Botrytis cinerea. The phys...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ren W,Sang C,Shi D,Song X,Zhou M,Chen C

    更新日期:2018-08-01 00:00:00

  • MAL63 codes for a positive regulator of maltose fermentation in Saccharomyces cerevisiae.

    abstract::Genetic analysis of the MAL6 locus has previously yielded mal6 mutants which fall into a single complementation group and which are noninducible for maltase and maltose permease. However, the strains used in these studies contained additional partially functional copies of MAL1 (referred to as MAL1g) and MAL3 (referre...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Chang YS,Dubin RA,Perkins E,Forrest D,Michels CA,Needleman RB

    更新日期:1988-09-01 00:00:00

  • Regulation of bacterial gene expression by ribosome stalling and rescuing.

    abstract::Ribosome is responsible for protein synthesis and is able to monitor the sequence and structure of the nascent peptide. Such ability plays an important role in determining overall gene expression profile of the bacteria through ribosome stalling and rescuing. In this review, we briefly summarize our current understand...

    journal_title:Current genetics

    pub_type: 杂志文章,评审


    authors: Jin Y,Jin S,Wu W

    更新日期:2016-05-01 00:00:00

  • Sgs1 helicase is required for efficient PCNA monoubiquitination and translesion DNA synthesis in Saccharomyces cerevisiae.

    abstract::DNA-damage tolerance (DDT) is employed by eukaryotes to deal with replication blocks on the template strand, and is divided into two parallel pathways that are activated by sequential ubiquitination of proliferating cell nuclear antigen (PCNA) at the Lys164 residue. Rad6-Rad18-mediated PCNA monoubiquitination promotes...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Li F,Ball LG,Fan L,Hanna M,Xiao W

    更新日期:2018-04-01 00:00:00

  • Inactivation of a single-2A phosphoprotein phosphatase is lethal in Neurospora crassa.

    abstract::A PCR approach, employing the use of degenerate oligonucleotide mixtures, was used to isolate pph-1, a type-2A protein phosphatase (catalytic subunit)-encoding gene, from Neurospora crassa. The isolated single copy gene is 1327 nucleotides in length, contains four putative introns and encodes a 310 amino-acid polypept...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Yatzkan E,Yarden O

    更新日期:1995-10-01 00:00:00

  • Analysis of cell-cycle specific localization of the Rdi1p RhoGDI and the structural determinants required for Cdc42p membrane localization and clustering at sites of polarized growth.

    abstract::The Cdc42p GTPase regulates multiple signal transduction pathways through its interactions with downstream effectors. Specific functional domains within Cdc42p are required for guanine-nucleotide binding, interactions with downstream effectors, and membrane localization. However, little is known about how Cdc42p is cl...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Richman TJ,Toenjes KA,Morales SE,Cole KC,Wasserman BT,Taylor CM,Koster JA,Whelihan MF,Johnson DI

    更新日期:2004-06-01 00:00:00

  • Functional characterization of SOR1, a gene required for resistance to photosensitizing toxins in the fungus Cercospora nicotianae.

    abstract::The Cercospora nicotianae SOR1 gene is required for resistance to singlet oxygen-generating photosensitizers. SOR1 was characterized in the wild-type and in five photosensitizer-sensitive mutant strains which are complemented to photosensitizer resistance by transformation with SOR1. Sequence analysis determined that ...

    journal_title:Current genetics

    pub_type: 杂志文章


    authors: Ehrenshaft M,Chung KR,Jenns AE,Daub ME

    更新日期:1999-01-01 00:00:00