Abstract:
:By functional complementation of the auxotrophic requirements for choline of a cdg1, cho2 double-mutant, by transformation with a genomic DNA library in a high copy number plasmid, two different types of complementing DNA inserts were identified. One type of insert was earlier shown to represent the CHO2 structural gene. In this report we describe the molecular and biochemical chemical characterization of the second type of complementing activity. The transcript encoded by the cloned gene was about 1000-nt in length and was regulated in response to the soluble phospholipid precursors, inositol and choline. A gene disruption resulted in no obvious growth phenotype at 23 degrees C or 30 degrees C, but in a lack of growth at 37 degrees C in the presence of monomethylethanolamine. Null-mutants exhibited an inositol-secretion phenotype, indicative of mutations in the lipid biosynthetic pathway. Complementation analysis, biochemical analysis of the phospholipid methylation pathway in vivo, and comparison of the restriction pattern of the cloned gene to published sequences, unequivocally identified the cloned gene as the OPI3 gene, encoding phospholipid-N-methyltransferase in yeast. When present in multiple copies the OPI3 gene efficiently suppresses the phospholipid methylation defect of a cho2 mutation. As a result of impaired synthesis of phosphatidylcholine, the INO1-deregulation phenotype is abolished in cho2 mutants transformed with the OPI3 gene on a high copy number plasmid.(ABSTRACT TRUNCATED AT 250 WORDS)
journal_name
Curr Genetjournal_title
Current geneticsauthors
Preitschopf W,Lückl H,Summers E,Henry SA,Paltauf F,Kohlwein SDdoi
10.1007/BF00352006subject
Has Abstractpub_date
1993-02-01 00:00:00pages
95-101issue
2eissn
0172-8083issn
1432-0983journal_volume
23pub_type
杂志文章相关文献
CURRENT GENETICS文献大全abstract::The ornithine transaminase (otaA) gene of Aspergillus nidulans has been cloned by transformation of the A. nidulans pro-ota- mutant strain with a cosmid gene library. The otaA gene contains two introns and potentially codes for a 453-aa-long protein. The deduced amino-acid sequence is homologous to known ornithine tra...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050440
更新日期:1999-03-01 00:00:00
abstract::In fungi, sexual reproduction primarily depends on the interaction between peptide pheromones and their receptors. Most ascomycete fungi produce two classes of peptide mating pheromones, a simple peptide and a modified peptide. These peptides are recognized by their corresponding receptors on the surface of cells of t...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-019-00968-w
更新日期:2019-10-01 00:00:00
abstract::We have analysed two new genes, YBR1007 and YBR1015, discovered during the systematic sequencing of chromosome II of S. cerevisiae. YBR1007 shows strong similarities to amino-acid permeases, in particular the high-affinity proline permeases of S. cerevisiae and A. nidulans. The number and position of the predicted mem...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326297
更新日期:1994-07-01 00:00:00
abstract::Enzymatic and genetic characterization of cpd-1 and cpd-2, which exhibit rhythmic conidiation in liquid media and on solid media, were described with band (bd) strain as a reference. Cpd-1 and cpd-2 showed reduced growth in orthophosphate-free cyclic 3',5'-AMP media, while bd showed wild-type level of growth in the me...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00798749
更新日期:1985-01-01 00:00:00
abstract::Replication-coupled (RC) nucleosome assembly is an essential process in eukaryotic cells to maintain chromatin structure during DNA replication. The deposition of newly-synthesized H3/H4 histones during DNA replication is facilitated by specialized histone chaperones. CAF-1 is an important histone chaperone complex an...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-020-01113-8
更新日期:2020-10-06 00:00:00
abstract::The Hansenula polymorpha per6-210 mutant is impaired in respect of growth on methanol (Mut-) and is characterized by aberrant peroxisome formation. The functionally complementing DNA fragment contains two open reading frames. The first encodes dihydroxyacetone kinase (DAK), a cytosolic enzyme essential for formaldehyd...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050360
更新日期:1998-07-01 00:00:00
abstract::Expression of pho1-encoded acid phosphatase of Schizosaccharomyces pombe has been reported to be regulated by phosphate. In this communication we show that it is also regulated by adenine. Starving adenine auxotrophic strains for adenine leads to a drastic increase of the enzymatic activity while adenine represses thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00317923
更新日期:1992-10-01 00:00:00
abstract::We recently discovered the novel non-chromosomal determinant in Saccharomyces cerevisiae [NSI(+)] (nonsense suppression inducer), which causes omnipotent nonsense suppression in strains where the Sup35 N-terminal domain is deleted. [NSI(+)] possesses yeast prion features and does not correspond to previously identifie...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-011-0363-1
更新日期:2012-02-01 00:00:00
abstract::Mitochondrial intron content is variable in the yeast Kluyveromyces lactis. Strains can be divided into three classes depending on the structure of the cytochrome oxidase subunit 1 (COX1) gene: (1) those containing intron K1 cox1.1, (2) those containing K1 cox1.2, 3 and 4 and, (3) those that contain all four introns. ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00312773
更新日期:1991-07-01 00:00:00
abstract::Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050118
更新日期:1996-07-31 00:00:00
abstract::Bands of dsRNA were detected in five out of seven isolates of the entomopathogenic fungus Metarhizium flavoviride. The identity of these bands was proven by RNase and S1-nuclease treatments. The transference of dsRNA between isolates (from CG291 to CG442) was successfully carried out through forced heterokaryons. Isog...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050477
更新日期:1999-08-01 00:00:00
abstract::Microorganisms have the ability to adapt and respond to different environmental conditions, whether they are stressful or not. Although the detection and/or responding mechanisms are often unknown, a large number of proteins may participate in signal transduction pathways involved in environmental stimulus to induce p...
journal_title:Current genetics
pub_type: 杂志文章,评审
doi:10.1007/s00294-017-0772-x
更新日期:2018-06-01 00:00:00
abstract::The meiotic recombination gene rec10, which encodes a region-specific activator of recombination in Schizosaccharomyces pombe, has been cloned by genetic complementation and its nucleotide sequence determined. The rec10 gene was identified in a 5.6-kb cloned fragment by partial-deletion and insertion experiments. The ...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311213
更新日期:1995-04-01 00:00:00
abstract::Mycorrhizal ascomycetes are ecologically and commercially important fungi that have proved impervious to genetic transformation so far. We report here on the successful transient transformation of Tuber borchii, an ectomycorrhizal ascomycete that colonizes a variety of trees and produces highly prized hypogeous fruitb...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-005-0579-z
更新日期:2005-07-01 00:00:00
abstract::In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxid...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00420609
更新日期:1986-01-01 00:00:00
abstract::Chloroplast DNAs from six different laboratory collections of Euglena gracilis "strain Z" and var. bacillaris were analyzed with restriction endonucleases EcoRI and Bam HI. The most variable portion of the organelle genome is the region containing the ribosomal cistrons. Intraspecific differences occur in both ribosom...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00429820
更新日期:1981-07-01 00:00:00
abstract::Trichoderma reesei was transformed to hygromycin B resistance using a novel vector, which contains the E. coli hygromycin B phosphotransferase gene (hph) fused between promoter and terminator elements of the homologous Trichoderma pki1 (coding for pyruvate kinase) and cbh2 (coding for cellobiohydrolase II) genes, resp...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351679
更新日期:1994-06-01 00:00:00
abstract::The cytoplasmic virus-like element pWR1A from Debaryomyces robertsiae encodes a toxin (DrT) with similarities to the Pichia acaciae killer toxin PaT, which acts by importing a toxin subunit (PaOrf2) with tRNA anticodon nuclease activity into target cells. As for PaT, loss of the tRNA methyltransferase Trm9 or overexpr...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-014-0426-1
更新日期:2014-08-01 00:00:00
abstract::Chloroplast genes are typically organized into polycistronic transcription units that give rise to complex sets of mono- and oligo-cistronic overlapping RNAs through a series of processing steps. The psbB operon contains genes for the PSII (psbB, psbT, psbH) and cytochrome b(6)f (petB and petD) complexes which are nee...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s002940050487
更新日期:1999-09-01 00:00:00
abstract::The nucleotide sequences of the plastidal psbD genes of Bumilleriopsis filiformis and Porphyridium purpureum (encoding the D2 protein of photosystem II) are reported in this paper. The Bumilleriopsis sequence clusters together with Porphyridium when a most parsimonious protein tree of D2 sequences is constructed. A co...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351854
更新日期:1993-11-01 00:00:00
abstract::Chitin is a component of the yeast cell wall which is localized to the septum between mother and daughter cells. Previous work in Saccharomyces cerevisiae has shown that this organism possesses three chitin synthases, 1, 2, and 3. Disruption experiments have shown that loss of chitin synthase 2 has a more profound eff...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00352007
更新日期:1993-02-01 00:00:00
abstract::The influence of the DNA repair gene PSO3 on photoactivated psoralen-induced meiotic recombination, gene conversion, reverse mutation, and on survival, was assayed in diploid strains of Saccharomyces cerevisiae homozygous for the wild-type or the pso3-1 mutant allele. Sporulation was normal in the pso3-1 diploid. Wild...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00712961
更新日期:1994-01-01 00:00:00
abstract::The mating type (MAT) locus of the lentil pathogen, Ascochyta lentis, was cloned and characterized using thermal asymmetric interlaced and inverse PCR with primers designed to the HMG-box of Ascochyta rabiei. A multiplex PCR assay for mating type was developed based on MAT idiomorph and flanking sequences. Primers wer...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0085-y
更新日期:2006-09-01 00:00:00
abstract::In order to study the mechanism responsible for the uniparental transmission of the mitochondrial genome in crosses between Chlamydomonas reinhardtii and C. smithii, we have analyzed the fate of mitochondrial DNA during gametogenesis, zygospore differentiation and sporulation by hybridization experiments. Both mt+ and...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00326238
更新日期:1991-08-01 00:00:00
abstract::Group-I introns, containing open reading frames (ORFs) that code for homing endonucleases, are widely distributed amongst eukaryotic organellar genomes. However, endonucleases of the GIY-YIG subclass have a restricted distribution in mitochondria and bacteriophages, and have never been observed in plastids. We have fo...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00311888
更新日期:1995-06-01 00:00:00
abstract::Genes are overexpressed when present in yeast cells on multicopy plasmids. Taking advantage of the protein amplification which results from this overexpression, a method has been developed for large scale detection of yeast genes on randomly cloned DNA sequences. It is based on the analysis, by two-dimensional gel ele...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00318208
更新日期:1990-11-01 00:00:00
abstract::We report the nucleotide sequence of three tRNA genes from maize mitochondria. The genes are located in two BamHI fragments, 3.55 and 5.7 kb long, adjacent to the S2 sequence in the maize mitochondrial genome. On the 3.55 kb BamHI fragment, we have characterized a tRNA(Cys)(GCA) gene. A strong sequence homology of thi...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00387771
更新日期:1988-03-01 00:00:00
abstract::The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/s00294-006-0097-7
更新日期:2006-12-01 00:00:00
abstract::Extraction of RNA from Tetrahymena mitochondrial ribosomes yields several RNA species, including a "large" 21S molecule, a "small" 14S molecule, a 7S molecule, and other smaller RNAs. The molecular weight of the 14S rRNA indicates that it is about 1,300 bases in length. We have sequenced the 14S rRNA gene and, by alig...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00384616
更新日期:1987-01-01 00:00:00
abstract::The mitochondrial leucyl-tRNA synthetase (mLRS) of Saccharomyces cerevisiae is involved in both mitochondrial protein synthesis and pre-mRNA splicing. We have created mutations in the regions HIGH, GWD and KMSKS, which are involved in ATP-, amino acid- and tRNA-binding respectively, and which have been conserved in th...
journal_title:Current genetics
pub_type: 杂志文章
doi:10.1007/BF00351744
更新日期:1992-07-01 00:00:00