Transformation of Trichoderma reesei based on hygromycin B resistance using homologous expression signals.

abstract：:As part of developing an ex planta model system for the study of maize plastid and mitochondrial gene expression, a series of established Black Mexican Sweet (BMS) suspension cell lines was characterized. Although the initial assumption was that their organelle biochemistry would be similar enough to normal in planta ...

journal_title：Current genetics

authors： Cahoon AB,Cunningham KA,Bollenbach TJ,Stern DB

更新日期：2003-11-01 00:00:00

abstract：:nam3 and R705, yeast nuclear omnipotent suppressors of mitochondrial mit- mutations, reverse the superimposed spectrum of trans-recessive splicing defects by affecting the protein composition of the small mitoribosomal subunit. Analysis of the suppressor's interaction suggests that suppression results from mutations i...

journal_title：Current genetics

authors： Boguta M,Mieszczak M,Zagórski W

更新日期：1988-02-01 00:00:00

abstract：:A previously unknown nuclear gene DSS-1 from Saccharomyces cerevisiae was cloned and sequenced. The gene was isolated as a multicopy suppressor of a disruption of the SUV-3 gene coding for a DEAD/H box protein involved in processing and turnover of mitochondrial transcripts. The DSS-1 gene codes for a 970 amino-acid p...

journal_title：Current genetics

authors： Dmochowska A,Golik P,Stepien PP

更新日期：1995-07-01 00:00:00

abstract：:A simple method was developed for the conversion of homothallic Saccharomyces cerevisiae yeast strains to heterothallism through HO gene disruption. An integrative ho::neo disrupted allele was constructed by cloning a dominant selectable marker, the bacterial neo gene, within the HO gene. Transformation of a homothall...

journal_title：Current genetics

authors： van Zyl WH,Lodolo EJ,Gericke M

更新日期：1993-01-01 00:00:00

abstract：:A DNA fragment which carries the leu1 gene encoding beta-isopropylmalate dehydrogenase in Schizosaccharomyces pombe has been isolated by complementation of an E. coli leuB mutation. This 1.5 kb DNA fragment complements not only the S. pombe leu1 mutation, but also the S. cerevisiae leu2 mutation. The nucleotide sequen...

journal_title：Current genetics

authors： Kikuchi Y,Kitazawa Y,Shimatake H,Yamamoto M

更新日期：1988-10-01 00:00:00

abstract：:Although 29 tRNA genes have been deduced from the complete nucleotide sequence of the mitochondrial genome from the liverwort Marchantia polymorpha, a tRNAIle gene decoding AUU and AUC codons is conspicuously absent. In order to address the question of the possible involvement of nuclear-encoded tRNA, we isolated and ...

journal_title：Current genetics

authors： Akashi K,Sakurai K,Hirayama J,Fukuzawa H,Ohyama K

更新日期：1996-07-31 00:00:00

abstract：:In Aspergillus nidulans, carbon catabolite repression (CCR) is mediated by the global repressor protein CreA. The deubiquitinating enzyme CreB is a component of the CCR network. Genetic interaction was confirmed using a strain containing complete loss-of-function alleles of both creA and creB. No direct physical inter...

journal_title：Current genetics

authors： Alam MA,Kelly JM

更新日期：2017-08-01 00:00:00

abstract：:Forces generated by molecular motors and the cytoskeleton move the nucleus and genome during many cellular processes, including cell migration and division. How these forces impact the genome, and whether cells regulate cytoskeletal forces to preserve genome integrity is unclear. We recently demonstrated that, in budd...

journal_title：Current genetics

authors： Estrem C,Moore JK

更新日期：2020-04-01 00:00:00

abstract：:We have constructed a gene coding for the 12-kDa intermediate form of the 2s methionine-rich protein from Bertholletia excelsa seeds. This protein, expressed intracellularly in yeast, is characterised by a 20-min half-life. By adding 11 amino acids corresponding to the peroxisome-targeting sequence (PTSc) of luciferas...

journal_title：Current genetics

authors： Nicaud JM,Raynal A,Beyou A,Merkamm M,Ito H,Labat N

更新日期：1994-11-01 00:00:00

abstract：:A naturally occurring single base-pair G to A transition, creating a 10/11 near-match close to the essential 11 base-pair core consensus of ARS1, was used to investigate the importance of near-match sequences. The 10/11 near-match can not substitute for the core consensus since an ARS- phenotype is observed when the c...

journal_title：Current genetics

authors： Kirpekar F,Gulløv K

更新日期：1992-09-01 00:00:00

abstract：:Mycorrhizal ascomycetes are ecologically and commercially important fungi that have proved impervious to genetic transformation so far. We report here on the successful transient transformation of Tuber borchii, an ectomycorrhizal ascomycete that colonizes a variety of trees and produces highly prized hypogeous fruitb...

journal_title：Current genetics

authors： Grimaldi B,de Raaf MA,Filetici P,Ottonello S,Ballario P

更新日期：2005-07-01 00:00:00

abstract：:Similarity searches with Didymium iridis mitochondrial genomic DNA identified six possible ribosomal protein-coding regions, however, each region contained stop codons that would need to be removed by RNA editing to produce functional transcripts. RT-PCR was used to amplify these regions from total RNA for cloning and...

journal_title：Current genetics

authors： Hendrickson PG,Silliker ME

更新日期：2010-06-01 00:00:00

abstract：:The yeast OMP decarboxylase gene has been cloned in the phage fd in both orientations to obtain pure DNA of each strand. With these probes the transcription of each strand of the yeast gene was measured in wild type yeast, either repressed or induced, in a yeast strain transformed by an hybrid plasmid containing the O...

journal_title：Current genetics

authors： Hubert JC,Bach ML,Lacroute F

更新日期：1980-10-01 00:00:00

abstract：:Spindle pole body (SPB; the microtubule organizing center in yeast) duplication is essential to form a bipolar spindle. The duplicated SPBs must then separate and migrate to opposite sides of the nucleus. We identified a novel functional relationship in SPB separation between the microtubule stabilizing protein Bik1p/...

journal_title：Current genetics

authors： Strawn LA,True HL

更新日期：2006-12-01 00:00:00

abstract：:Studies on replicative and chronological aging in Saccharomyces cerevisiae have greatly advanced our understanding of how longevity is regulated in all eukaryotes. Chronological lifespan (CLS) of yeast is defined as the age-dependent viability of non-dividing cell populations. A number of nutrient sensing and signal t...

journal_title：Current genetics

authors： Zhang N,Cao L

更新日期：2017-10-01 00:00:00

abstract：:The isoprenoid pathway of the ectomycorrhizal fungus Tuber borchii Vittad is investigated to better understand the molecular mechanisms at work, in particular during the maturation of the complex ascomata (the so-called "truffles"). Three T. borchii genes coding for the most important regulatory enzymes of the isopren...

journal_title：Current genetics

authors： Guidi C,Zeppa S,Annibalini G,Pierleoni R,Guescini M,Buffalini M,Zambonelli A,Stocchi V

更新日期：2006-12-01 00:00:00

abstract：:URA4, the gene coding for orotidine monophosphate decarboxylase (OMPdecase), has been cloned from the fission yeast by homologous complementation and restricted in an Escherichia coli-Schizosaccharomyces pombe (E. coli-S. pombe) replicative plasmid to a 1.76 kb HindIII fragment. This plasmid is maintained at a high co...

journal_title：Current genetics

authors： Bach ML

更新日期：1987-01-01 00:00:00

abstract：:A screening of haploid yeast strains for enhanced resistance to nitrogen mustard (HN2) yielded a recessive mutant allele, hnm1, that conferred hyper-resistance (HYR) to HN2. Diploids, homo- or heterozygous for the HNM1 locus, exhibit normal wild-type like resistance while homozygosity for hnm1 leads to the phenotype H...

journal_title：Current genetics

authors： Haase E,Brendel M

更新日期：1990-10-01 00:00:00

abstract：:The A-pathway of development in the basidiomycete fungus Schizophyllum commune may be activated by either of two mating-type loci, A alpha and A beta. A alpha consists of two multiallelic genes, Y and Z. Y contains a putative homeodomain; Z contains a homeodomain-related region. Non-self combinations of Y and Z form h...

journal_title：Current genetics

authors： Shen GP,Park DC,Ullrich RC,Novotny CP

更新日期：1996-01-01 00:00:00

abstract：:During evolution, most of the bacterial genes from the ancestral endosymbiotic alpha-proteobacteria at the origin of mitochondria have been either lost or transferred to the nuclear genome. A crucial evolutionary step was the establishment of macromolecule import systems to allow the come back of proteins and RNAs int...

journal_title：Current genetics

authors： Duchêne AM,Pujol C,Maréchal-Drouard L

更新日期：2009-02-01 00:00:00

abstract：:Most organisms prefer to utilize glucose as a carbon source. Accordingly, the expression of genes involved in the catabolism of other carbon sources is repressed by the presence of glucose in a process known as (carbon) catabolite repression. However, much less is known about the relationships between "poor" carbon so...

journal_title：Current genetics

authors： Simpson-Lavy K,Kupiec M

更新日期：2019-12-01 00:00:00

abstract：:La France disease of the cultivated mushroom, Agaricus bisporus, is known to be associated with the presence of a number of dsRNA segments. The nucleotide sequences of the dsRNAs M2 (1.3 kb), M1 (1.55 kb) and L3 (2.8 kb), invariably associated with the disease, were determined. Putative coding sequences for proteins w...

journal_title：Current genetics

authors： Harmsen MC,Tolner B,Kram A,Go SJ,de Haan A,Wessels JG

更新日期：1991-07-01 00:00:00

abstract：:This paper reports the mapping of both mature and precursor Paracentrotus lividus mitochondrial transcripts. Several mtRNAs were found to have 5' and 3' termini which differ from those inferred through DNA sequencing (Cantatore et al. 1989). The 3' ends of the two rRNAs (12S and 16S) overlap with the downstream transc...

journal_title：Current genetics

authors： Cantatore P,Roberti M,Loguercio Polosa P,Mustich A,Gadaleta MN

更新日期：1990-03-01 00:00:00

abstract：:Primary transcripts in higher plant cell organelles undergo a series of essential RNA processing steps, including 5'- and 3'-terminal maturation, intron splicing and RNA editing. Most of the protein factors involved in these RNA processing mechanisms are still unknown. Also, little is known about the environmental and...

journal_title：Current genetics

authors： Karcher D,Bock R

更新日期：2002-04-01 00:00:00

abstract：:Seven MboI fragments spanning the mitochondrial apocytochrome b gene in Saccharomyces cerevisiae strain D273-10B were cloned in the BamHI site of the integrative yeast vector YIp5 and the capacity for autonomous replication was subsequently assayed in yeast. The positive correlation found between the ars-like activity...

journal_title：Current genetics

authors： Delouya D,Bonjardim CA,Nobrega FG

更新日期：1987-01-01 00:00:00

abstract：:The cDNA coding for the endoglucanase EGII of P. janthinellum was cloned and sequenced. The open reading frame comprises 1230 nucleotides and the deduced amino-acid sequence shows an overall homology of 63% with the T. reesei egl2. The cellulose-binding domain of EGII represents a typical member of the A family of cel...

journal_title：Current genetics

authors： Mernitz G,Koch A,Henrissat B,Schulz G

更新日期：1996-04-01 00:00:00

abstract：:A class of large site-specific deletions (del-B) occurs with exceptionally-high frequencies of 10(-3) in the mitochondrial COX1 gene of Mn(2+)-treated yeast cells. This work shows that del-B deletions are associated with COX1 intron aI1. All five deletion mutants studied have their upstream end at the authentic 3' spl...

journal_title：Current genetics

authors： Weiller GF

更新日期：1994-11-01 00:00:00

abstract：:A main function of ectomycorrhizas, a symbiosis between certain soil fungi and fine roots of woody plants, is the exchange of plant-derived carbohydrates for fungus-derived nutrients. As it is required in large amounts, nitrogen is of special interest. A gene (AmAMT2) coding for a putative fungal ammonium importer was...

journal_title：Current genetics

authors： Willmann A,Weiss M,Nehls U

更新日期：2007-02-01 00:00:00

abstract：:In order to analyse gene expression during fruit body development of the ectomychorrizal fungus Tuber borchii Vittad., a modified differential display procedure was set up. The procedure used is easier and faster than the traditional one and generates reproducible cDNA banding patterns that can be resolved on a standa...

journal_title：Current genetics

authors： Zeppa S,Guidi C,Zambonelli A,Potenza L,Vallorani L,Pierleoni R,Sacconi C,Stocchi V

更新日期：2002-12-01 00:00:00

abstract：:The DNA sequence of a 7.5-kbp region from the maize cmsT mt genome was determined. Nucleotide-sequence analysis of this region reveals the presence of a ct-like trnI (CAT) gene, orf31 and orf48 which are respectively homologous to the trnI (CAT) gene in the maize and rice chloroplast genomes, and to orf28 and orf64 in...

journal_title：Current genetics

authors： Zheng D,Nielsen BL,Daniell H

更新日期：1997-08-01 00:00:00