Abstract:
:In order to study resistance to DNA damaging agents, yeast DNA segments conferring hyperresistance in this organism to such genotoxic agents were selected for among yeast cells transformed by a yeast genome library based on the multi-copy vector plasmid YEp13. Genetic variants hyperresistant to 4-nitroquinoline-N-oxide, formaldehyde, and alkylating agents were isolated and the respective hyperresistance determinants shown to co-segregate with the vector plasmid. Phenotypical characterization indicated different degrees of resistance, few cases of cross-resistance and differing structural stability of the cloned DNA. By transfer to E. coli and subsequent retransformation of yeast a number of plasmids was shown to stably carry the genetic information for hyperresistance.
journal_name
Curr Genetjournal_title
Current geneticsauthors
Ruhland A,Brendel M,Haynes RHdoi
10.1007/BF00420609subject
Has Abstractpub_date
1986-01-01 00:00:00pages
211-5issue
3eissn
0172-8083issn
1432-0983journal_volume
11pub_type
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